{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T21:11:18Z","timestamp":1760044278931},"reference-count":35,"publisher":"American Chemical Society (ACS)","issue":"20","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biochemistry"],"published-print":{"date-parts":[[2005,5,1]]},"DOI":"10.1021\/bi047477y","type":"journal-article","created":{"date-parts":[[2005,5,17]],"date-time":"2005-05-17T04:32:59Z","timestamp":1116304379000},"page":"7526-7534","source":"Crossref","is-referenced-by-count":20,"title":["Engineering Intracellular CMP-Sialic Acid Metabolism into Insect Cells and Methods to Enhance Its Generation"],"prefix":"10.1021","volume":"44","author":[{"given":"Karthik","family":"Viswanathan","sequence":"first","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering and Department of Biology, Johns Hopkins University, Baltimore, Maryland, 21218, and Charit\u00e9Universit\u00e4tsmedizin Berlin, Campus Benjamin Franklin, Institut f\u00fcr Biochemie und Molekularbiologie, Arnimallee 22, 14195 Berlin-Dahlem, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Someet","family":"Narang","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering and Department of Biology, Johns Hopkins University, Baltimore, Maryland, 21218, and Charit\u00e9Universit\u00e4tsmedizin Berlin, Campus Benjamin Franklin, Institut f\u00fcr Biochemie und Molekularbiologie, Arnimallee 22, 14195 Berlin-Dahlem, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephan","family":"Hinderlich","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering and Department of Biology, Johns Hopkins University, Baltimore, Maryland, 21218, and Charit\u00e9Universit\u00e4tsmedizin Berlin, Campus Benjamin Franklin, Institut f\u00fcr Biochemie und Molekularbiologie, Arnimallee 22, 14195 Berlin-Dahlem, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan C.","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering and Department of Biology, Johns Hopkins University, Baltimore, Maryland, 21218, and Charit\u00e9Universit\u00e4tsmedizin Berlin, Campus Benjamin Franklin, Institut f\u00fcr Biochemie und Molekularbiologie, Arnimallee 22, 14195 Berlin-Dahlem, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael J.","family":"Betenbaugh","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering and Department of Biology, Johns Hopkins University, Baltimore, Maryland, 21218, and Charit\u00e9Universit\u00e4tsmedizin Berlin, Campus Benjamin Franklin, Institut f\u00fcr Biochemie und Molekularbiologie, Arnimallee 22, 14195 Berlin-Dahlem, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"316","published-online":{"date-parts":[[2005,4,26]]},"reference":[{"key":"bi047477yb00001\/bi047477yb00001_1","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1093\/glycob\/3.2.97","volume":"3","author":"Varki A.","year":"1993","journal-title":"Glycobiology"},{"key":"bi047477yb00002\/bi047477yb00002_1","volume-title":"Cell. Mol. Life Sci. 54, 1330\u22121349.","author":"Traving C.","year":"1998"},{"key":"bi047477yb00003\/bi047477yb00003_1","doi-asserted-by":"crossref","unstructured":"Schauer, R., Kelm, S., Reuter, G., Roggentin, P., and Shaw, L. (1995) inBiology of the Sialic Acids(1995, Ed.) pp 7\u221267, Plenum Press, New York.","DOI":"10.1007\/978-1-4757-9504-2_2"},{"key":"bi047477yb00004\/bi047477yb00004_1","doi-asserted-by":"crossref","DOI":"10.1016\/S0021-9258(18)54895-X","volume":"266","author":"Rens-Domiano S.","year":"1991","journal-title":"J. Biol. Chem."},{"key":"bi047477yb00005\/bi047477yb00005_1","volume":"270","author":"Pirie-Shepherd S. R.","year":"1995","journal-title":"J. Biol. Chem."},{"key":"bi047477yb00006\/bi047477yb00006_1","volume-title":"Cancer Res. 56, 2237\u22122244.","author":"Fukuda M.","year":"1996"},{"key":"bi047477yb00007\/bi047477yb00007_1","volume-title":"Glycobiology 4, 665\u2212674.","author":"Takano R.","year":"1994"},{"key":"bi047477yb00008\/bi047477yb00008_1","volume-title":"Glycobiology 10, 837\u2212847.","author":"Ailor E.","year":"2000"},{"key":"bi047477yb00009\/bi047477yb00009_1","volume-title":"Nat. Biotechnol. 14, 1288\u22121292.","author":"Jarvis D. L.","year":"1996"},{"key":"bi047477yb00010\/bi047477yb00010_1","volume-title":"Glycobiology 8, 473\u2212480.","author":"Hollister J. R.","year":"1998"},{"key":"bi047477yb00011\/bi047477yb00011_1","volume-title":"Biotechnol. Bioeng. 63, 559\u2212572.","author":"Hooker A. D.","year":"1999"},{"key":"bi047477yb00012\/bi047477yb00012_1","volume-title":"Anal. Biochem. 293, 129\u2212137.","author":"Tomiya N.","year":"2001"},{"key":"bi047477yb00013\/bi047477yb00013_1","doi-asserted-by":"crossref","DOI":"10.1023\/A:1012452705349","volume":"18","author":"Lawrence S. M.","year":"2001","journal-title":"Glycoconjugate J."},{"key":"bi047477yb00014\/bi047477yb00014_1","volume":"275","author":"Lawrence S. M.","year":"2000","journal-title":"J. Biol. Chem."},{"key":"bi047477yb00015\/bi047477yb00015_1","volume-title":"Biotechnol. Bioeng. 58, 642\u2212648.","author":"Gu X.","year":"1998"},{"key":"bi047477yb00016\/bi047477yb00016_1","doi-asserted-by":"crossref","DOI":"10.1074\/jbc.272.39.24313","volume":"272","author":"Hinderlich S.","year":"1997","journal-title":"J. Biol. Chem."},{"key":"bi047477yb00017\/bi047477yb00017_1","doi-asserted-by":"crossref","DOI":"10.1074\/jbc.274.40.28771","volume":"274","author":"Effertz K.","year":"1999","journal-title":"J. Biol. Chem."},{"key":"bi047477yb00018\/bi047477yb00018_1","volume-title":"Proc. Natl. Acad. Sci. U.S.A. 47","author":"Roseman S.","year":"1961"},{"key":"bi047477yb00019\/bi047477yb00019_1","volume-title":"Proc. Natl. Acad. Sci. U.S.A. 95","author":"M\u00fcnster A. K.","year":"1998"},{"key":"bi047477yb00020\/bi047477yb00020_1","first-page":"259","volume-title":"H.-J.","author":"Reutter W.","year":"1997"},{"key":"bi047477yb00021\/bi047477yb00021_1","volume-title":"Biol. Chem. 382, 291\u2212297.","author":"Hinderlich S.","year":"2001"},{"key":"bi047477yb00022\/bi047477yb00022_1","volume-title":"Glycobiology 13, 497\u2212507.","author":"Aumiller J. J.","year":"2003"},{"key":"bi047477yb00023\/bi047477yb00023_1","volume-title":"Biochemistry 42, 15215\u221215225.","author":"Viswanathan K.","year":"2003"},{"key":"bi047477yb00024\/bi047477yb00024_1","volume":"327","author":"Kikuchi K.","year":"1973","journal-title":"Biochim. Biophys. Acta"},{"key":"bi047477yb00025\/bi047477yb00025_1","volume-title":"Proc. Natl. Acad. Sci. U.S.A. 52","author":"Kornfeld S.","year":"1964"},{"key":"bi047477yb00026\/bi047477yb00026_1","volume-title":"Protein Expression Purif. 35, 387\u2212396.","author":"Blume A.","year":"2004"},{"key":"bi047477yb00027\/bi047477yb00027_1","volume-title":"Baculovirus Expression Vectors:\u2009 A Laboratory Manual","author":"O'Reilly D. R.","year":"1992"},{"key":"bi047477yb00028\/bi047477yb00028_1","volume-title":"Protein Expression Purif. 22, 234\u2212241.","author":"Seo N.-S.","year":"2001"},{"key":"bi047477yb00029\/bi047477yb00029_1","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1093\/glycob\/11.1.1","volume":"11","author":"Hollister J. R.","year":"2001","journal-title":"Glycobiology"},{"key":"bi047477yb00030\/bi047477yb00030_1","volume-title":"Anal. Biochem. 179, 162\u2212166.","author":"Hara S.","year":"1989"},{"key":"bi047477yb00031\/bi047477yb00031_1","doi-asserted-by":"crossref","DOI":"10.1086\/302411","volume":"64","author":"Seppala R.","year":"1999","journal-title":"Am. J. Hum. Genet."},{"key":"bi047477yb00032\/bi047477yb00032_1","volume":"264","author":"Weiss P.","year":"1989","journal-title":"J. Biol. Chem."},{"key":"bi047477yb00033\/bi047477yb00033_1","volume-title":"Proc. Natl. Acad. Sci. U.S.A. 92","author":"Sarkar A. K.","year":"1995"},{"key":"bi047477yb00034\/bi047477yb00034_1","volume-title":"Science 284, 1372\u22121376.","author":"Keppler O. T.","year":"1999"},{"key":"bi047477yn00001\/bi047477yn00001_1","volume-title":"N-acetylneuraminic acid","author":"Abbreviations"}],"container-title":["Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/bi047477y","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,18]],"date-time":"2021-10-18T08:20:30Z","timestamp":1634545230000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/doi\/10.1021\/bi047477y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,4,26]]},"references-count":35,"journal-issue":{"issue":"20","published-print":{"date-parts":[[2005,5,1]]}},"alternative-id":["10.1021\/bi047477y"],"URL":"https:\/\/doi.org\/10.1021\/bi047477y","relation":{},"ISSN":["0006-2960","1520-4995"],"issn-type":[{"value":"0006-2960","type":"print"},{"value":"1520-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,4,26]]}}}