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Chem."},{"key":"ref23\/cit23","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/S0076-6879(97)76066-X","volume":"276","author":"Otwinowski Z.","year":"1997","journal-title":"Methods Enzymol."},{"key":"ref24\/cit24","first-page":"760","volume":"50","author":"Bailey S.","year":"1994","journal-title":"Acta Crystallogr."},{"key":"ref25\/cit25","first-page":"2126","volume":"60","author":"Emsley P.","year":"2004","journal-title":"Acta Crystallogr."},{"key":"ref26\/cit26","doi-asserted-by":"crossref","first-page":"622","DOI":"10.1107\/S0021889893002729","volume":"26","author":"Howlin P.","year":"1993","journal-title":"J. Appl. Crystallogr."},{"key":"ref27\/cit27","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1002\/prot.340120407","volume":"12","author":"Morris A. 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