{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T17:32:47Z","timestamp":1788370367430,"version":"build-2803163510"},"reference-count":55,"publisher":"American Chemical Society (ACS)","issue":"13","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biochemistry"],"published-print":{"date-parts":[[2012,4,3]]},"DOI":"10.1021\/bi2017987","type":"journal-article","created":{"date-parts":[[2012,3,7]],"date-time":"2012-03-07T16:07:59Z","timestamp":1331136479000},"page":"2775-2784","source":"Crossref","is-referenced-by-count":16,"title":["A Highly Conserved Interaction Involving the Middle Residue of the SXN Active-Site Motif Is Crucial for Function of Class B Penicillin-Binding Proteins: Mutational and Computational Analysis of PBP 2 from <i>N. gonorrhoeae<\/i>"],"prefix":"10.1021","volume":"51","author":[{"given":"Joshua","family":"Tomberg","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brenda","family":"Temple","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alena","family":"Fedarovich","sequence":"additional","affiliation":[{"name":"Department\rof Biochemistry, Medical University of South Carolina, Charleston, South\rCarolina 29425, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Davies","sequence":"additional","affiliation":[{"name":"Department\rof Biochemistry, Medical University of South Carolina, Charleston, South\rCarolina 29425, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert A.","family":"Nicholas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2012,3,22]]},"reference":[{"key":"ref1\/cit1","unstructured":"CDC (2009) Sexually Transmitted Disease Surveillance, 2007."},{"key":"ref2\/cit2","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1097\/00007435-199219020-00001","volume":"19","author":"Wasserheit J. N.","year":"1992","journal-title":"Sex Transm. Dis."},{"key":"ref3\/cit3","doi-asserted-by":"crossref","first-page":"1868","DOI":"10.1016\/S0140-6736(97)02190-9","volume":"349","author":"Cohen M. S.","year":"1997","journal-title":"Lancet"},{"key":"ref4\/cit4","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1136\/sti.2010.045377","volume":"86","author":"Golparian D.","year":"2010","journal-title":"Sex. Transm. Infect."},{"key":"ref5\/cit5","doi-asserted-by":"crossref","first-page":"3538","DOI":"10.1128\/AAC.00325-11","volume":"55","author":"Ohnishi M.","year":"2011","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref6\/cit6","doi-asserted-by":"crossref","first-page":"148","DOI":"10.3201\/eid1701.100397","volume":"17","author":"Ohnishi M.","year":"2011","journal-title":"Emerg. Infect. Dis."},{"key":"ref7\/cit7","doi-asserted-by":"crossref","unstructured":"Unemo, M., Golparian, D., Nicholas, R., Ohnishi, M., Gallay, A., and Sednaoui, P.High-level cefixime- and ceftriaxone-resistantN. gonorrhoeaein Europe (France): novelpenAmosaic allele in a successful international clone causes treatment failure.Antimicrob. Agents Chemother. 2011, not supplied.","DOI":"10.1128\/AAC.05760-11"},{"key":"ref8\/cit8","first-page":"245","volume-title":"Neisseria: Molecular Mechanisms of Pathogenesis","author":"Shafer W. M.","year":"2010"},{"key":"ref9\/cit9","first-page":"111","volume":"38","author":"Cannon J. G.","year":"1984","journal-title":"Annu. Rev. Genet."},{"key":"ref10\/cit10","doi-asserted-by":"crossref","first-page":"740","DOI":"10.1128\/jb.124.2.740-749.1975","volume":"124","author":"Sparling P. F.","year":"1975","journal-title":"J. Bacteriol."},{"key":"ref11\/cit11","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1128\/AAC.49.1.137-143.2005","volume":"49","author":"Ito M.","year":"2005","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref12\/cit12","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1111\/j.1574-6976.2007.00094.x","volume":"32","author":"Vollmer W.","year":"2008","journal-title":"FEMS Microbiol. Rev."},{"key":"ref13\/cit13","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.tim.2009.12.004","volume":"18","author":"Vollmer W.","year":"2010","journal-title":"Trends Microbiol."},{"key":"ref14\/cit14","doi-asserted-by":"crossref","first-page":"1133","DOI":"10.1073\/pnas.54.4.1133","volume":"54","author":"Tipper D. J.","year":"1965","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"ref15\/cit15","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1146\/annurev.mi.45.100191.000345","volume":"45","author":"Ghuysen J. M.","year":"1991","journal-title":"Annu. Rev. Microbiol."},{"key":"ref16\/cit16","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1128\/MMBR.62.4.1079-1093.1998","volume":"62","author":"Goffin C.","year":"1998","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref17\/cit17","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1111\/j.1574-6976.2008.00105.x","volume":"32","author":"Sauvage E.","year":"2008","journal-title":"FEMS Microbiol. Rev."},{"key":"ref18\/cit18","doi-asserted-by":"crossref","first-page":"4886","DOI":"10.1074\/jbc.M706181200","volume":"283","author":"Job V.","year":"2008","journal-title":"J. Biol. Chem."},{"key":"ref19\/cit19","doi-asserted-by":"crossref","first-page":"16463","DOI":"10.1074\/jbc.M313492200","volume":"279","author":"Pernot L.","year":"2004","journal-title":"J. Biol. Chem."},{"key":"ref20\/cit20","doi-asserted-by":"crossref","first-page":"1202","DOI":"10.1074\/jbc.M805761200","volume":"284","author":"Powell A. J.","year":"2009","journal-title":"J. Biol. Chem."},{"key":"ref21\/cit21","doi-asserted-by":"crossref","first-page":"8062","DOI":"10.1021\/bi101167x","volume":"49","author":"Tomberg J.","year":"2010","journal-title":"Biochemistry"},{"key":"ref22\/cit22","doi-asserted-by":"crossref","first-page":"44448","DOI":"10.1074\/jbc.M305948200","volume":"278","author":"Chesnel L.","year":"2003","journal-title":"J. Biol. Chem."},{"key":"ref23\/cit23","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1038\/332173a0","volume":"332","author":"Spratt B. G.","year":"1988","journal-title":"Nature"},{"key":"ref24\/cit24","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1093\/infdis\/128.3.321","volume":"128","author":"Maness M. J.","year":"1973","journal-title":"J. Infect. Dis."},{"key":"ref25\/cit25","doi-asserted-by":"crossref","first-page":"1274","DOI":"10.1128\/jb.85.6.1274-1279.1963","volume":"85","author":"Kellogg D. S.","year":"1963","journal-title":"J. Bacteriol."},{"key":"ref26\/cit26","doi-asserted-by":"crossref","first-page":"2117","DOI":"10.1128\/AAC.01604-06","volume":"51","author":"Lindberg R.","year":"2007","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref27\/cit27","doi-asserted-by":"crossref","first-page":"3911","DOI":"10.1128\/jb.173.12.3911-3913.1991","volume":"173","author":"Elkins C.","year":"1991","journal-title":"J. Bacteriol."},{"key":"ref28\/cit28","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.jmb.2006.03.023","volume":"359","author":"Powell A. J.","year":"2006","journal-title":"J. Mol. Biol."},{"key":"ref29\/cit29","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/0378-1119(89)90358-2","volume":"77","author":"Ho S. N.","year":"1989","journal-title":"Gene"},{"issue":"2","key":"ref30\/cit30","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1128\/AAC.19.2.316","volume":"19","author":"Barbour A. G.","year":"1981","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref31\/cit31","doi-asserted-by":"crossref","first-page":"3744","DOI":"10.1128\/AAC.00304-09","volume":"53","author":"Zhao S.","year":"2009","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref32\/cit32","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1128\/AAC.46.3.769-777.2002","volume":"46","author":"Ropp P. A.","year":"2002","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref33\/cit33","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1007\/978-94-011-2928-2_5","volume-title":"The Chemistry of \u03b2-Lactams","author":"Frere J. M.","year":"1992"},{"key":"ref34\/cit34","doi-asserted-by":"crossref","first-page":"14614","DOI":"10.1021\/bi0350607","volume":"42","author":"Stefanova M. E.","year":"2003","journal-title":"Biochemistry"},{"key":"ref35\/cit35","doi-asserted-by":"crossref","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","volume":"25","author":"Altschul S. F.","year":"1997","journal-title":"Nucleic Acids Res."},{"key":"ref36\/cit36","doi-asserted-by":"crossref","first-page":"1792","DOI":"10.1093\/nar\/gkh340","volume":"32","author":"Edgar R. C.","year":"2004","journal-title":"Nucleic Acids Res."},{"key":"ref37\/cit37","doi-asserted-by":"crossref","first-page":"6097","DOI":"10.1093\/nar\/18.20.6097","volume":"18","author":"Schneider T. D.","year":"1990","journal-title":"Nucleic Acids Res."},{"key":"ref38\/cit38","doi-asserted-by":"crossref","first-page":"1188","DOI":"10.1101\/gr.849004","volume":"14","author":"Crooks G. E.","year":"2004","journal-title":"Genome Res."},{"issue":"6","key":"ref39\/cit39","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1111\/j.1365-2958.1990.tb00664.x","volume":"4","author":"Brannigan J. A.","year":"1990","journal-title":"Mol. Microbiol."},{"key":"ref40\/cit40","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1006\/jmbi.2000.3740","volume":"299","author":"Gordon E.","year":"2000","journal-title":"J. Mol. Biol."},{"key":"ref41\/cit41","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.jmb.2010.02.046","volume":"398","author":"Fedarovich A.","year":"2010","journal-title":"J. Mol. Biol."},{"key":"ref42\/cit42","first-page":"870","volume":"9","author":"Lim D.","year":"2002","journal-title":"Nat. Struct. Biol."},{"key":"ref43\/cit43","doi-asserted-by":"crossref","first-page":"45106","DOI":"10.1074\/jbc.M107608200","volume":"276","author":"Dessen A.","year":"2001","journal-title":"J. Biol. Chem."},{"key":"ref44\/cit44","doi-asserted-by":"crossref","first-page":"1771","DOI":"10.1074\/jbc.M511506200","volume":"281","author":"Carapito R.","year":"2006","journal-title":"J. Biol. Chem."},{"key":"ref45\/cit45","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1016\/j.jmb.2005.10.030","volume":"355","author":"Contreras-Martel C.","year":"2006","journal-title":"J. Mol. Biol."},{"key":"ref46\/cit46","doi-asserted-by":"crossref","first-page":"1701","DOI":"10.1110\/ps.062112106","volume":"15","author":"Lovering A. L.","year":"2006","journal-title":"Protein Sci."},{"key":"ref47\/cit47","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1073\/pnas.0407186102","volume":"102","author":"Macheboeuf P.","year":"2005","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"ref48\/cit48","doi-asserted-by":"crossref","first-page":"1402","DOI":"10.1126\/science.1136611","volume":"315","author":"Lovering A. L.","year":"2007","journal-title":"Science"},{"key":"ref49\/cit49","doi-asserted-by":"crossref","first-page":"2034","DOI":"10.1016\/S0021-9258(19)77981-2","volume":"263","author":"Nicholas R. A.","year":"1988","journal-title":"J. Biol. Chem."},{"key":"ref50\/cit50","doi-asserted-by":"crossref","first-page":"52826","DOI":"10.1074\/jbc.M310177200","volume":"278","author":"Nicholas R. A.","year":"2003","journal-title":"J. Biol. Chem."},{"key":"ref51\/cit51","doi-asserted-by":"crossref","first-page":"1294","DOI":"10.1016\/S0021-9258(17)43602-7","volume":"259","author":"Amanuma H.","year":"1984","journal-title":"J. Biol. Chem."},{"key":"ref52\/cit52","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1074\/jbc.M004471200","volume":"276","author":"Davies C.","year":"2001","journal-title":"J. Biol. Chem."},{"key":"ref53\/cit53","doi-asserted-by":"crossref","first-page":"14345","DOI":"10.1021\/ja903773f","volume":"131","author":"Chen Y.","year":"2009","journal-title":"J. Am. Chem. Soc."},{"key":"ref54\/cit54","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1086\/515605","volume":"178","author":"Rybkine T.","year":"1998","journal-title":"J. Infect. Dis."},{"key":"ref55\/cit55","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1007\/s00018-002-8500-0","volume":"59","author":"Sauvage E.","year":"2002","journal-title":"Cell. Mol. Life Sci."}],"container-title":["Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/bi2017987","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,6]],"date-time":"2023-04-06T05:35:28Z","timestamp":1680759328000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/doi\/10.1021\/bi2017987"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,3,22]]},"references-count":55,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2012,4,3]]}},"alternative-id":["10.1021\/bi2017987"],"URL":"https:\/\/doi.org\/10.1021\/bi2017987","relation":{},"ISSN":["0006-2960","1520-4995"],"issn-type":[{"value":"0006-2960","type":"print"},{"value":"1520-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,3,22]]}}}