{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T16:01:02Z","timestamp":1790438462895,"version":"4.1.0"},"reference-count":53,"publisher":"American Chemical Society (ACS)","issue":"18","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biochemistry"],"published-print":{"date-parts":[[2012,5,8]]},"DOI":"10.1021\/bi300298v","type":"journal-article","created":{"date-parts":[[2012,4,19]],"date-time":"2012-04-19T15:08:06Z","timestamp":1334848086000},"page":"3881-3890","source":"Crossref","is-referenced-by-count":34,"title":["Type IV Secretion System Core Component VirB8 from <i>Brucella<\/i> Binds to the Globular Domain of VirB5 and to a Periplasmic Domain of VirB6"],"prefix":"10.1021","volume":"51","author":[{"given":"Ana Maria","family":"Villamil Giraldo","sequence":"first","affiliation":[{"name":"Department\rof Biochemistry, Universit\u00e9 de Montr\u00e9al, C.P. 6128, Succ. Centre-ville, Montr\u00e9al, QC H3C 3J7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Durga","family":"Sivanesan","sequence":"additional","affiliation":[{"name":"Department\rof Biochemistry, Universit\u00e9 de Montr\u00e9al, C.P. 6128, Succ. Centre-ville, Montr\u00e9al, QC H3C 3J7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Carle","sequence":"additional","affiliation":[{"name":"Department Biologie I, Bereich\rMikrobiologie, Ludwig-Maximilians-Universit\u00e4t, Maria-Ward-Strasse 1a, D-80638 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Athanasios","family":"Paschos","sequence":"additional","affiliation":[{"name":"Department of Biology, McMaster University, 1280 Main Street West, Hamilton, ON L8S\r4K1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark A.","family":"Smith","sequence":"additional","affiliation":[{"name":"Department\rof Biochemistry, Universit\u00e9 de Montr\u00e9al, C.P. 6128, Succ. Centre-ville, Montr\u00e9al, QC H3C 3J7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Plesa","sequence":"additional","affiliation":[{"name":"Department\rof Microbiology and Immunology, McGill University, 3775 University Street, Montr\u00e9al, QC H3A 2B4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Coulton","sequence":"additional","affiliation":[{"name":"Department\rof Microbiology and Immunology, McGill University, 3775 University Street, Montr\u00e9al, QC H3A 2B4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Baron","sequence":"additional","affiliation":[{"name":"Department\rof Biochemistry, Universit\u00e9 de Montr\u00e9al, C.P. 6128, Succ. Centre-ville, Montr\u00e9al, QC H3C 3J7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2012,4,26]]},"reference":[{"key":"ref1\/cit1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1365-2958.2004.04168.x","volume":"53","author":"Llosa M.","year":"2004","journal-title":"Mol. Microbiol."},{"key":"ref2\/cit2","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1038\/nrmicro753","volume":"1","author":"Cascales E.","year":"2003","journal-title":"Nat. Rev. Microbiol."},{"key":"ref3\/cit3","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1146\/annurev.micro.58.030603.123630","volume":"59","author":"Christie P. J.","year":"2005","journal-title":"Annu. Rev. Microbiol."},{"key":"ref4\/cit4","doi-asserted-by":"crossref","first-page":"26349","DOI":"10.1074\/jbc.M502347200","volume":"280","author":"Yuan Q.","year":"2005","journal-title":"J. Biol. Chem."},{"key":"ref5\/cit5","doi-asserted-by":"crossref","first-page":"3469","DOI":"10.1099\/mic.0.28326-0","volume":"151","author":"H\u00f6ppner C.","year":"2005","journal-title":"Microbiology"},{"key":"ref6\/cit6","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.mib.2003.11.001","volume":"7","author":"Celli J.","year":"2004","journal-title":"Curr. Opin. Microbiol."},{"key":"ref7\/cit7","doi-asserted-by":"crossref","first-page":"17228","DOI":"10.1073\/pnas.0405843101","volume":"101","author":"Cascales E.","year":"2004","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"ref8\/cit8","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1128\/jb.179.2.453-462.1997","volume":"179","author":"Dang T. A.","year":"1997","journal-title":"J. Bacteriol."},{"key":"ref9\/cit9","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1046\/j.1365-2958.1999.01436.x","volume":"32","author":"Dang T. A.","year":"1999","journal-title":"Mol. Microbiol."},{"key":"ref10\/cit10","doi-asserted-by":"crossref","first-page":"2711","DOI":"10.1128\/JB.180.10.2711-2717.1998","volume":"180","author":"Lai E.-M.","year":"1998","journal-title":"J. Bacteriol."},{"key":"ref11\/cit11","doi-asserted-by":"crossref","first-page":"22548","DOI":"10.1074\/jbc.274.32.22548","volume":"274","author":"Eisenbrandt R.","year":"1999","journal-title":"J. Biol. Chem."},{"key":"ref12\/cit12","doi-asserted-by":"crossref","first-page":"3486","DOI":"10.1128\/JB.187.10.3486-3495.2005","volume":"187","author":"Jakubowski S. J.","year":"2005","journal-title":"J. Bacteriol."},{"key":"ref13\/cit13","doi-asserted-by":"crossref","first-page":"7485","DOI":"10.1128\/JB.181.24.7485-7492.1999","volume":"181","author":"Schmidt-Eisenlohr H.","year":"1999","journal-title":"J. Bacteriol."},{"key":"ref14\/cit14","doi-asserted-by":"crossref","first-page":"5563","DOI":"10.1128\/JB.181.18.5563-5571.1999","volume":"181","author":"Schmidt-Eisenlohr H.","year":"1999","journal-title":"J. Bacteriol."},{"key":"ref15\/cit15","doi-asserted-by":"crossref","first-page":"11405","DOI":"10.1073\/pnas.172390699","volume":"99","author":"Krall L.","year":"2002","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"ref16\/cit16","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1111\/j.1365-2958.2004.04378.x","volume":"55","author":"Judd P. K.","year":"2005","journal-title":"Mol. Microbiol."},{"key":"ref17\/cit17","doi-asserted-by":"crossref","first-page":"15947","DOI":"10.1073\/pnas.2535211100","volume":"100","author":"Yeo H.-J.","year":"2003","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"ref18\/cit18","doi-asserted-by":"crossref","first-page":"4596","DOI":"10.1073\/pnas.0408927102","volume":"102","author":"Terradot L.","year":"2005","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"ref19\/cit19","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.jmb.2006.04.060","volume":"360","author":"Hare S.","year":"2006","journal-title":"J. Mol. Biol."},{"key":"ref20\/cit20","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1038\/nrmicro2218","volume":"7","author":"Fronzes R.","year":"2009","journal-title":"Nat. Rev. Microbiol."},{"key":"ref21\/cit21","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1038\/35054586","volume":"409","author":"Gomis-Ruth F. X.","year":"2001","journal-title":"Nature"},{"key":"ref22\/cit22","doi-asserted-by":"crossref","first-page":"1461","DOI":"10.1016\/S1097-2765(00)00142-8","volume":"6","author":"Yeo H. J.","year":"2000","journal-title":"Mol. Cell"},{"key":"ref23\/cit23","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1038\/nature08588","volume":"462","author":"Chandran V.","year":"2009","journal-title":"Nature"},{"key":"ref24\/cit24","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1126\/science.1166101","volume":"323","author":"Fronzes R.","year":"2009","journal-title":"Science"},{"key":"ref25\/cit25","doi-asserted-by":"crossref","first-page":"11493","DOI":"10.1073\/pnas.172390299","volume":"99","author":"Ward D. V.","year":"2002","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"ref26\/cit26","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1128\/JB.182.3.758-763.2000","volume":"182","author":"Das A.","year":"2000","journal-title":"J. Bacteriol."},{"key":"ref27\/cit27","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1139\/o06-148","volume":"84","author":"Baron C.","year":"2006","journal-title":"Biochem. Cell Biol."},{"key":"ref28\/cit28","doi-asserted-by":"crossref","first-page":"7252","DOI":"10.1073\/pnas.0600862103","volume":"103","author":"Paschos A.","year":"2006","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"ref29\/cit29","doi-asserted-by":"crossref","first-page":"4483","DOI":"10.1021\/bi902201y","volume":"49","author":"Sivanesan D.","year":"2010","journal-title":"Biochemistry"},{"key":"ref30\/cit30","doi-asserted-by":"crossref","first-page":"4505","DOI":"10.1128\/JB.182.16.4505-4511.2000","volume":"182","author":"Hapfelmeier S.","year":"2000","journal-title":"J. Bacteriol."},{"key":"ref31\/cit31","doi-asserted-by":"crossref","first-page":"2867","DOI":"10.1128\/JB.185.9.2867-2878.2003","volume":"185","author":"Jakubowski S. J.","year":"2003","journal-title":"J. Bacteriol."},{"key":"ref32\/cit32","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1016\/j.jmb.2004.06.052","volume":"341","author":"Jakubowski S. J.","year":"2004","journal-title":"J. Mol. Biol."},{"key":"ref33\/cit33","doi-asserted-by":"crossref","first-page":"1170","DOI":"10.1126\/science.1095211","volume":"304","author":"Cascales E.","year":"2004","journal-title":"Science"},{"key":"ref34\/cit34","doi-asserted-by":"crossref","first-page":"3766","DOI":"10.1099\/mic.0.2007\/010462-0","volume":"153","author":"Aly K. A.","year":"2007","journal-title":"Microbiology"},{"key":"ref35\/cit35","doi-asserted-by":"crossref","first-page":"2582","DOI":"10.1073\/pnas.0511216103","volume":"103","author":"Bailey S.","year":"2006","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"ref36\/cit36","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Maniatis T. A.","year":"1982"},{"key":"ref37\/cit37","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1006\/abio.1996.0060","volume":"234","author":"Ansaldi M.","year":"1996","journal-title":"Anal. Biochem."},{"key":"ref38\/cit38","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/j.jmb.2005.12.039","volume":"357","author":"Carter D. M.","year":"2006","journal-title":"J. Mol. Biol."},{"key":"ref39\/cit39","doi-asserted-by":"crossref","first-page":"1439","DOI":"10.1002\/pmic.200300680","volume":"4","author":"Mandava S.","year":"2004","journal-title":"Proteomics"},{"key":"ref40\/cit40","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","volume":"227","author":"Laemmli U. K.","year":"1970","journal-title":"Nature"},{"key":"ref41\/cit41","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1016\/0003-2697(87)90587-2","volume":"166","author":"Sch\u00e4gger H.","year":"1987","journal-title":"Anal. Biochem."},{"key":"ref42\/cit42","volume-title":"Antibodies: A laboratory manual","author":"Harlow E.","year":"1988"},{"key":"ref43\/cit43","doi-asserted-by":"crossref","first-page":"5752","DOI":"10.1073\/pnas.95.10.5752","volume":"95","author":"Karimova G.","year":"1998","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"ref44\/cit44","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1016\/0003-2697(92)90381-G","volume":"206","author":"Wang Z. X.","year":"1992","journal-title":"Anal. Biochem."},{"key":"ref45\/cit45","first-page":"submitted for p","author":"Smith M. A.","year":"2012","journal-title":"Proc. Natl. Acad. Sci. U.S.A.,"},{"key":"ref46\/cit46","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1046\/j.1365-2958.2000.01876.x","volume":"36","author":"Kumar R. B.","year":"2000","journal-title":"Mol. Microbiol."},{"key":"ref47\/cit47","doi-asserted-by":"crossref","first-page":"11498","DOI":"10.1073\/pnas.0505290102","volume":"102","author":"Judd P. K.","year":"2005","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"ref48\/cit48","doi-asserted-by":"crossref","first-page":"e00218-00211","DOI":"10.1128\/mBio.00218-11","volume":"2","author":"Aguilar J.","year":"2011","journal-title":"MBio"},{"key":"ref49\/cit49","doi-asserted-by":"crossref","first-page":"3758","DOI":"10.1073\/pnas.0914940107","volume":"107","author":"Aguilar J.","year":"2010","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"ref50\/cit50","doi-asserted-by":"crossref","first-page":"3483","DOI":"10.1099\/mic.0.28337-0","volume":"151","author":"Judd P. K.","year":"2005","journal-title":"Microbiology"},{"key":"ref51\/cit51","doi-asserted-by":"crossref","first-page":"2097","DOI":"10.1128\/JB.00907-10","volume":"193","author":"Sivanesan D.","year":"2011","journal-title":"J. Bacteriol."},{"key":"ref52\/cit52","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1016\/j.tim.2008.07.001","volume":"16","author":"Backert S.","year":"2008","journal-title":"Trends Microbiol."},{"key":"ref53\/cit53","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.bbamcr.2004.02.010","volume":"1694","author":"Sauer F. G.","year":"2004","journal-title":"Biochim. Biophys. Acta"}],"container-title":["Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/bi300298v","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,23]],"date-time":"2023-04-23T14:51:10Z","timestamp":1682261470000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/doi\/10.1021\/bi300298v"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,4,26]]},"references-count":53,"journal-issue":{"issue":"18","published-print":{"date-parts":[[2012,5,8]]}},"alternative-id":["10.1021\/bi300298v"],"URL":"https:\/\/doi.org\/10.1021\/bi300298v","relation":{},"ISSN":["0006-2960","1520-4995"],"issn-type":[{"value":"0006-2960","type":"print"},{"value":"1520-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,4,26]]}}}