{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T09:14:00Z","timestamp":1788340440965,"version":"build-2803163510"},"reference-count":67,"publisher":"American Chemical Society (ACS)","issue":"48","license":[{"start":{"date-parts":[[2015,11,12]],"date-time":"2015-11-12T00:00:00Z","timestamp":1447286400000},"content-version":"vor","delay-in-days":365,"URL":"https:\/\/pubs.acs.org\/page\/policy\/authorchoice_termsofuse.html"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["P30CA046934"],"award-info":[{"award-number":["P30CA046934"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["1R01Al109008-01"],"award-info":[{"award-number":["1R01Al109008-01"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006133","name":"Advanced Research Projects Agency - Energy","doi-asserted-by":"publisher","award":["DE-AC02-05CH11231"],"award-info":[{"award-number":["DE-AC02-05CH11231"]}],"id":[{"id":"10.13039\/100006133","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/na","name":"Colorado Center for Drug Discovery","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,12,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Staphylococcus aureus is one of the most common nosocomial sources of soft-tissue and skin infections and has more recently become prevalent in the community setting as well. Since the use of penicillins to combat S. aureus infections in the 1940s, the bacterium has been notorious for developing resistances to antibiotics, such as methicillin-resistant Staphylococcus aureus (MRSA). With the persistence of MRSA as well as many other drug resistant bacteria and parasites, there is a growing need to focus on new pharmacological targets. Recently, class II fructose 1,6-bisphosphate aldolases (FBAs) have garnered attention to fill this role. Regrettably, scarce biochemical data and no structural data are currently available for the class II FBA found in MRSA (SaFBA). With the recent finding of a flexible active site zinc-binding loop (Z-Loop) in class IIa FBAs and its potential for broad spectrum class II FBA inhibition, the lack of information regarding this feature of class IIb FBAs, such as SaFBA, has been limiting for further Z-loop inhibitor development. Therefore, we elucidated the crystal structure of SaFBA to 2.1 \u00c5 allowing for a more direct structural analysis of SaFBA. Furthermore, we determined the KM for one of SaFBA\u2019s substrates, fructose 1,6-bisphosphate, as well as performed mode of inhibition studies for an inhibitor that takes advantage of the Z-loop\u2019s flexibility. Together the data offers insight into a class IIb FBA from a pervasively drug resistant bacterium and a comparison of Z-loops and other features between the different subtypes of class II FBAs.<\/jats:p>","DOI":"10.1021\/bi501141t","type":"journal-article","created":{"date-parts":[[2014,11,12]],"date-time":"2014-11-12T18:08:52Z","timestamp":1415815732000},"page":"7604-7614","source":"Crossref","is-referenced-by-count":17,"title":["Structural and Functional Characterization of Methicillin-Resistant\n                    <i>Staphylococcus aureus\u2019s<\/i>\n                    Class IIb Fructose 1,6-Bisphosphate\nAldolase"],"prefix":"10.1021","volume":"53","author":[{"given":"Glenn C.","family":"Capodagli","sequence":"first","affiliation":[{"name":"University of Denver , , , ,","place":["Denver, Colorado, United States, 80208"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen A.","family":"Lee","sequence":"additional","affiliation":[{"name":"University of Denver , , , ,","place":["Denver, Colorado, United States, 80208"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyle J.","family":"Boehm","sequence":"additional","affiliation":[{"name":"University of Denver , , , ,","place":["Denver, Colorado, United States, 80208"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristin M.","family":"Brady","sequence":"additional","affiliation":[{"name":"University of Denver , , , ,","place":["Denver, Colorado, United States, 80208"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott D.","family":"Pegan","sequence":"additional","affiliation":[{"name":"University of Georgia , , , ,","place":["Athens, Georgia, United States, 30602"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2014,11,12]]},"reference":[{"key":"2026090204183593900_cit1","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1016\/j.mcna.2013.02.005","article-title":"Methicillin-resistant\nStaphylococcus aureus infections","volume":"97","author":"Pottinger","year":"2013","journal-title":"Med. Clin.\nNorth Am."},{"key":"2026090204183593900_cit2","doi-asserted-by":"crossref","first-page":"1980","DOI":"10.1001\/jamainternmed.2013.10408","article-title":"High-density\nlivestock operations, crop field application of manure, and risk of\ncommunity-associated methicillin-resistant Staphylococcus aureus infection\nin Pennsylvania","volume":"173","author":"Casey","year":"2013","journal-title":"JAMA Intern. Med."},{"key":"2026090204183593900_cit3","unstructured":"Centers for Disease Control and Prevention.\n          \n          Active\nBacterial Core Surveillance (ABCs) Report Emerging Infections Program\nNetwork Methicillin-Resistant Staphylococcus aureus, 2011; 2011."},{"key":"2026090204183593900_cit4","first-page":"1970","article-title":"National burden of invasive methicillin-resistant Staphylococcus\naureus infections, United States, 2011","volume":"173","author":"Dantes","year":"2013","journal-title":"JAMA\nIntern. 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U.S.A."},{"key":"2026090204183593900_cit26","doi-asserted-by":"crossref","first-page":"4678","DOI":"10.1073\/pnas.0730515100","article-title":"Essential Bacillus subtilis genes","volume":"100","author":"Kobayashi","year":"2003","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"2026090204183593900_cit27","doi-asserted-by":"crossref","first-page":"2833","DOI":"10.1073\/pnas.0511100103","article-title":"An ordered, nonredundant\nlibrary of Pseudomonas aeruginosa strain PA14 transposon insertion\nmutants","volume":"103","author":"Liberati","year":"2006","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"2026090204183593900_cit28","doi-asserted-by":"crossref","first-page":"1371","DOI":"10.1128\/EC.00115-06","article-title":"Effects\nof depleting the essential\ncentral metabolic enzyme fructose-1,6-bisphosphate aldolase on the\ngrowth and viability of Candida albicans: implications for antifungal\ndrug target discovery","volume":"5","author":"Rodaki","year":"2006","journal-title":"Eukaryotic Cell"},{"key":"2026090204183593900_cit29","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1046\/j.1365-2958.2003.03425.x","article-title":"Genes required\nfor mycobacterial\ngrowth defined by high density mutagenesis","volume":"48","author":"Sassetti","year":"2003","journal-title":"Mol. 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