{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T04:56:26Z","timestamp":1791262586157,"version":"4.1.0"},"reference-count":68,"publisher":"American Chemical Society (ACS)","issue":"2","license":[{"start":{"date-parts":[[2015,12,23]],"date-time":"2015-12-23T00:00:00Z","timestamp":1450828800000},"content-version":"vor","delay-in-days":365,"URL":"https:\/\/pubs.acs.org\/page\/policy\/authorchoice_termsofuse.html"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["GM49758"],"award-info":[{"award-number":["GM49758"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,1,20]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The X-ray crystal structure of an arginase-like protein from the parasitic protozoan Trypanosoma brucei, designated TbARG, is reported at 1.80 and 2.38 \u00c5 resolution in its reduced and oxidized forms, respectively. The oxidized form of TbARG is a disulfide-linked hexamer that retains the overall architecture of a dimer of trimers in the reduced form. Intriguingly, TbARG does not contain metal ions in its putative active site, and amino acid sequence comparisons indicate that all but one of the residues required for coordination to the catalytically obligatory binuclear manganese cluster in other arginases are substituted here with residues incapable of metal ion coordination. Therefore, the structure of TbARG is the first of a member of the arginase\/deacetylase superfamily that is not a metalloprotein. Although we show that metal binding activity is easily reconstituted in TbARG by site-directed mutagenesis and confirmed in X-ray crystal structures, it is curious that this protein and its parasitic orthologues evolved away from metal binding function. Knockout of the TbARG gene from the genome demonstrated that its function is not essential to cultured bloodstream-form T. brucei, and metabolomics analysis confirmed that the enzyme has no role in the conversion of l-arginine to l-ornithine in these cells. While the molecular function of TbARG remains enigmatic, the fact that the T. brucei genome encodes only this protein and not a functional arginase indicates that the parasite must import l-ornithine from its host to provide a source of substrate for ornithine decarboxylase in the polyamine biosynthetic pathway, an active target for the development of antiparasitic drugs.<\/jats:p>","DOI":"10.1021\/bi501366a","type":"journal-article","created":{"date-parts":[[2014,12,23]],"date-time":"2014-12-23T10:54:10Z","timestamp":1419332050000},"page":"458-471","source":"Crossref","is-referenced-by-count":26,"title":["Crystal Structure of an Arginase-like Protein from\n                    <i>Trypanosoma brucei<\/i>\n                    That Evolved without a Binuclear Manganese\nCluster"],"prefix":"10.1021","volume":"54","author":[{"given":"Yang","family":"Hai","sequence":"first","affiliation":[{"name":"University of Pennsylvania , , , ,","place":["Philadelphia, Pennsylvania, United States, 19104-6323"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduard J.","family":"Kerkhoven","sequence":"additional","affiliation":[{"name":"Chalmers University of Technology , , ,","place":["Go\u0308teborg, Sweden, SE-412 96"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael P.","family":"Barrett","sequence":"additional","affiliation":[{"name":"University of Glasgow , , ,","place":["Glasgow, United Kingdom, G12 8TA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David W.","family":"Christianson","sequence":"additional","affiliation":[{"name":"University of Pennsylvania , , , ,","place":["Philadelphia, Pennsylvania, United States, 19104-6323"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2014,12,23]]},"reference":[{"key":"2026090207225785900_cit1","doi-asserted-by":"crossref","first-page":"1469","DOI":"10.1016\/S0140-6736(03)14694-6","article-title":"The trypanosomiases","volume":"362","author":"Barrett","year":"2003","journal-title":"Lancet"},{"key":"2026090207225785900_cit2","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/S0140-6736(09)60829-1","article-title":"Human African trypanosomiasis","volume":"375","author":"Brun","year":"2010","journal-title":"Lancet"},{"key":"2026090207225785900_cit3","doi-asserted-by":"crossref","first-page":"1377","DOI":"10.1016\/S0140-6736(06)68591-7","article-title":"The rise\nand fall of sleeping sickness","volume":"367","author":"Barrett","year":"2006","journal-title":"Lancet"},{"key":"2026090207225785900_cit4","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1016\/j.pt.2003.09.002","article-title":"Chemotherapy\nof human African trypanosomiasis: Current and future prospects","volume":"19","author":"Fairlamb","year":"2003","journal-title":"Trends Parasitol."},{"key":"2026090207225785900_cit5","doi-asserted-by":"crossref","first-page":"677","DOI":"10.2217\/fmb.11.44","article-title":"Development of novel\ndrugs for human African trypanosomiasis","volume":"6","author":"Brun","year":"2011","journal-title":"Future\nMicrobiol."},{"key":"2026090207225785900_cit6","doi-asserted-by":"crossref","first-page":"842","DOI":"10.1017\/S0031182012000169","article-title":"Update on field use\nof the available drugs for the\nchemotherapy of human African trypanosomiasis","volume":"139","author":"Simarro","year":"2012","journal-title":"Parasitology"},{"key":"2026090207225785900_cit7","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1006\/expr.1998.4237","article-title":"Trypanosoma brucei brucei: Characterization of an\nODC null bloodstream form mutant and the action of \u03b1-difluoromethylornithine","volume":"88","author":"Li","year":"1998","journal-title":"Exp. Parasitol."},{"key":"2026090207225785900_cit8","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1042\/bst0310415","article-title":"Polyamine biosynthetic enzymes as\ndrug targets in parasitic protozoa","volume":"31","author":"Heby","year":"2003","journal-title":"Biochem.\nSoc. Trans."},{"key":"2026090207225785900_cit9","doi-asserted-by":"crossref","first-page":"15174","DOI":"10.1021\/bi9915115","article-title":"X-ray structure\nof ornithine decarboxylase from Trypanosoma brucei: The native structure and the structure in complex with \u03b1-difluoromethylornithine","volume":"38","author":"Grishin","year":"1999","journal-title":"Biochemistry"},{"key":"2026090207225785900_cit10","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1126\/science.6775372","article-title":"Polyamine\nmetabolism: A potential therapeutic target in trypanosomes","volume":"210","author":"Bacchi","year":"1980","journal-title":"Science"},{"key":"2026090207225785900_cit11","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1038\/nrd2243","article-title":"Targeting polyamine metabolism and function in cancer\nand other hyperproliferative diseases","volume":"6","author":"Casero","year":"2007","journal-title":"Nat.\nRev. Drug Discovery"},{"key":"2026090207225785900_cit12","doi-asserted-by":"crossref","first-page":"1602S","DOI":"10.1093\/jn\/137.6.1602S","article-title":"Arginine metabolism: Boundaries of our knowledge","volume":"137","author":"Morris","year":"2007","journal-title":"J. Nutr."},{"key":"2026090207225785900_cit13","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1146\/annurev.biochem.68.1.33","article-title":"Catalysis by metal-activated hydroxide in zinc and\nmanganese metalloenzymes","volume":"68","author":"Christianson","year":"1999","journal-title":"Annu. Rev. Biochem."},{"key":"2026090207225785900_cit14","first-page":"407","article-title":"Arginase: A binuclear manganese\nmetalloenzyme","volume-title":"Manganese and Its Role in\nBiological Processes","author":"Ash","year":"1999"},{"key":"2026090207225785900_cit15","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1021\/ar040183k","article-title":"Arginase:\nStructure, mechanism, and physiological role in male and female sexual\narousal","volume":"38","author":"Christianson","year":"2005","journal-title":"Acc. Chem. Res."},{"key":"2026090207225785900_cit16","doi-asserted-by":"crossref","first-page":"23668","DOI":"10.1074\/jbc.M402042200","article-title":"Arginase plays a pivotal role in\npolyamine precursor\nmetabolism in Leishmania: Characterization of gene\ndeletion mutants","volume":"279","author":"Roberts","year":"2004","journal-title":"J. Biol. Chem."},{"key":"2026090207225785900_cit17","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1016\/j.sbi.2011.08.004","article-title":"Structure,\nmechanism, and inhibition of histone deacetylases and related metalloenzymes","volume":"21","author":"Lombardi","year":"2011","journal-title":"Curr. Opin. Struct. Biol."},{"key":"2026090207225785900_cit18","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/S0076-6879(97)76066-X","article-title":"Processing of X-ray diffraction data collected in oscillation mode","volume":"276","author":"Otwinowski","year":"1997","journal-title":"Methods Enzymol."},{"key":"2026090207225785900_cit19","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1107\/S0021889804018047","article-title":"HKL2MAP: A graphical user interface for phasing with\nSHELX programs","volume":"37","author":"Pape","year":"2004","journal-title":"J. Appl. Crystallogr."},{"key":"2026090207225785900_cit20","first-page":"213","article-title":"PHENIX: A comprehensive Python-based\nsystem for macromolecular structure solution","volume":"D66","author":"Adams","year":"2010","journal-title":"Acta Crystallogr."},{"key":"2026090207225785900_cit21","first-page":"486","article-title":"Features and development\nof Coot","volume":"D66","author":"Emsley","year":"2010","journal-title":"Acta Crystallogr."},{"key":"2026090207225785900_cit22","first-page":"458","article-title":"Likelihood-enhanced\nfast translation functions","volume":"D61","author":"McCoy","year":"2005","journal-title":"Acta Crystallogr."},{"key":"2026090207225785900_cit23","first-page":"235","article-title":"Overview\nof the CCP4 suite and current developments","volume":"D67","author":"Winn","year":"2011","journal-title":"Acta Crystallogr."},{"key":"2026090207225785900_cit24","first-page":"1124","article-title":"A statistic for local intensity differences: robustness\nto anisotropy and pseudo-centering and utility for detecting twinning","volume":"D59","author":"Padilla","year":"2003","journal-title":"Acta Crystallogr."},{"key":"2026090207225785900_cit25","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1107\/S0108767387009632","article-title":"Simple\nstatistics for intensity data from twinned specimens","volume":"A44","author":"Yeates","year":"1988","journal-title":"Acta Crystallogr."},{"key":"2026090207225785900_cit26","first-page":"375","article-title":"Structure determination of plastocyanin from a specimen\nwith a hemihedral twinning fraction of one-half","volume":"D49","author":"Redinbo","year":"1993","journal-title":"Acta Crystallogr."},{"key":"2026090207225785900_cit27","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1016\/S0076-6879(97)76068-3","article-title":"Detecting\nand overcoming crystal twinning","volume":"276","author":"Yeates","year":"1997","journal-title":"Methods Enzymol."},{"key":"2026090207225785900_cit28","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1038\/14929","article-title":"Arginase-boronic\nacid complex highlights a physiological role in erectile function","volume":"6","author":"Cox","year":"1999","journal-title":"Nat. Struct. Biol."},{"key":"2026090207225785900_cit29","doi-asserted-by":"crossref","first-page":"13058","DOI":"10.1073\/pnas.0504027102","article-title":"Crystal\nstructure of human arginase I at 1.29-\u00c5 resolution and exploration\nof inhibition in the immune response","volume":"102","author":"Di Costanzo","year":"2005","journal-title":"Proc.\nNatl. Acad. Sci. U.S.A."},{"key":"2026090207225785900_cit30","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1107\/S0021889892009944","article-title":"PROCHECK: A program\nto check the stereochemical quality of protein structures","volume":"26","author":"Laskowski","year":"1993","journal-title":"J. Appl. Crystallogr."},{"key":"2026090207225785900_cit31","doi-asserted-by":"crossref","first-page":"774","DOI":"10.1016\/j.jmb.2007.05.022","article-title":"Inference of macromolecular assemblies from crystalline\nstate","volume":"372","author":"Krissinel","year":"2007","journal-title":"J. Mol. Biol."},{"key":"2026090207225785900_cit32","doi-asserted-by":"crossref","first-page":"2212","DOI":"10.1038\/nprot.2007.321","article-title":"The use of differential\nscanning\nfluorimetry to detect ligand interactions that promote protein stability","volume":"2","author":"Niesen","year":"2007","journal-title":"Nat. Protoc."},{"key":"2026090207225785900_cit33","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1016\/S0021-9258(18)51085-1","article-title":"Colorimetric\ndetermination of urea","volume":"157","author":"Archibald","year":"1945","journal-title":"J. Biol. Chem."},{"key":"2026090207225785900_cit34","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1006\/abio.2000.4805","article-title":"A colorimetric\n96-well microtiter plate assay for the\ndetermination of enzymatically formed citrulline","volume":"286","author":"Knipp","year":"2000","journal-title":"Anal. Biochem."},{"key":"2026090207225785900_cit35","doi-asserted-by":"crossref","first-page":"9294","DOI":"10.1021\/bi401352h","article-title":"Formiminoglutamase\nfrom Trypanosoma cruzi is an arginase-like manganese\nmetalloenzyme","volume":"52","author":"Hai","year":"2013","journal-title":"Biochemistry"},{"key":"2026090207225785900_cit36","doi-asserted-by":"crossref","first-page":"2768","DOI":"10.1128\/AAC.00044-13","article-title":"Metabolomics guides rational development\nof a simplified\ncell culture medium for drug screening against Trypanosoma brucei","volume":"57","author":"Creek","year":"2013","journal-title":"Antimicrob. Agents Chemother."},{"key":"2026090207225785900_cit37","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1046\/j.1365-2958.2002.03144.x","article-title":"J-binding protein increases\nthe level\nand retention of the unusual base J in trypanosome DNA","volume":"46","author":"Cross","year":"2002","journal-title":"Mol. Microbiol."},{"key":"2026090207225785900_cit38","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.molbiopara.2010.09.002","article-title":"Genome-wide RNAi screens\nin bloodstream form trypanosomes identify drug transporters","volume":"175","author":"Schumann Burkard","year":"2011","journal-title":"Mol. Biochem. Parasitol."},{"key":"2026090207225785900_cit39","doi-asserted-by":"crossref","first-page":"2352","DOI":"10.1128\/AAC.01551-10","article-title":"Trypanocidal furamidine analogues: Influence of pyridine\nnitrogens on trypanocidal activity, transport kinetics, and resistance\npatterns","volume":"55","author":"Ward","year":"2011","journal-title":"Antimicrob. Agents Chemother."},{"key":"2026090207225785900_cit40","doi-asserted-by":"crossref","first-page":"2059","DOI":"10.1007\/s00216-010-4139-0","article-title":"Towards an\nunbiased metabolic profiling of protozoan parasites: Optimization\nof a Leishmania sampling protocol for HILIC-Orbitrap\nanalysis","volume":"398","author":"t\u2019Kindt","year":"2010","journal-title":"Anal. Bioanal. Chem."},{"key":"2026090207225785900_cit41","doi-asserted-by":"crossref","first-page":"3447","DOI":"10.1002\/rcm.5247","article-title":"Semi-targeted\nanalysis of metabolites using capillary-flow ion chromatography coupled\nto high-resolution mass spectrometry","volume":"25","author":"Burgess","year":"2011","journal-title":"Rapid\nCommun. Mass Spectrom."},{"issue":"5","key":"2026090207225785900_cit42","doi-asserted-by":"crossref","first-page":"e1618","DOI":"10.1371\/journal.pntd.0001618","article-title":"Untargeted metabolomics reveals a lack of synergy between\nNifurtimox and Eflornithine against Trypanosoma brucei","volume":"6","author":"Vincent","year":"2012","journal-title":"PLoS Neglected Trop. Dis."},{"key":"2026090207225785900_cit43","doi-asserted-by":"crossref","first-page":"2786","DOI":"10.1021\/ac2000994","article-title":"PeakML\/mzMatch:\nA file format, Java library, R library, and tool-chain for mass spectrometry\ndata analysis","volume":"83","author":"Scheltema","year":"2011","journal-title":"Anal. Chem."},{"key":"2026090207225785900_cit44","doi-asserted-by":"crossref","first-page":"1048","DOI":"10.1093\/bioinformatics\/bts069","article-title":"IDEOM: An Excel interface for analysis of LC-MS-based\nmetabolomics data","volume":"28","author":"Creek","year":"2012","journal-title":"Bioinformatics"},{"key":"2026090207225785900_cit45","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1007\/s11306-007-0082-2","article-title":"Proposed minimum\nreporting standards for chemical analysis","volume":"3","author":"Sumner","year":"2007","journal-title":"Metabolomics"},{"key":"2026090207225785900_cit46","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.febslet.2004.07.055","article-title":"Rank products: A simple,\nyet powerful, new method to detect differentially regulated genes\nin replicated microarray experiments","volume":"573","author":"Breitling","year":"2004","journal-title":"FEBS Lett."},{"key":"2026090207225785900_cit47","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1016\/S0969-2126(99)80056-2","article-title":"Crystal\nstructures of Bacillus caldovelox arginase in complex\nwith substrate and inhibitors reveal new insights into activation,\ninhibition and catalysis in the arginase superfamily","volume":"7","author":"Bewley","year":"1999","journal-title":"Structure"},{"key":"2026090207225785900_cit48","unstructured":"Kumarevel, T. S., Karthe, P., Kuramitsu, S., and Yokoyama, S. (2009) Crystal structure of the arginase\nfrom Thermus thermophilus. PDB entry 2EF4,10.2210\/pdb2ef4\/pdb."},{"key":"2026090207225785900_cit49","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1042\/bj20020125","article-title":"Oligomeric structure of proclavaminic\nacid amidino hydrolase: Evolution of a hydrolytic enzyme in clavulanic\nacid biosynthesis","volume":"366","author":"Elkins","year":"2002","journal-title":"Biochem. J."},{"key":"2026090207225785900_cit50","doi-asserted-by":"crossref","first-page":"50505","DOI":"10.1074\/jbc.M409246200","article-title":"Crystal structure\nof agmatinase reveals structural conservation and inhibition mechanism\nof the ureohydrolase superfamily","volume":"279","author":"Ahn","year":"2004","journal-title":"J. Biol. Chem."},{"key":"2026090207225785900_cit51","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.jsb.2011.05.002","article-title":"Crystal\nstructures of Pseudomonas aeruginosa guanidinobutyrase\nand guanidinopropionase, members of the ureohydrolase superfamily","volume":"175","author":"Lee","year":"2011","journal-title":"J. Struct. Biol."},{"key":"2026090207225785900_cit52","doi-asserted-by":"crossref","first-page":"5600","DOI":"10.1021\/bi100390z","article-title":"Crystal structure of arginase from Plasmodium falciparum and implications for l-arginine\ndepletion in malarial infection","volume":"49","author":"Dowling","year":"2010","journal-title":"Biochemistry"},{"key":"2026090207225785900_cit53","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1038\/383554a0","article-title":"Structure\nof a unique binuclear manganese cluster in arginase","volume":"383","author":"Kanyo","year":"1996","journal-title":"Nature"},{"key":"2026090207225785900_cit54","doi-asserted-by":"crossref","first-page":"W545","DOI":"10.1093\/nar\/gkq366","article-title":"Dali server: Conservation mapping in 3D","volume":"38","author":"Holm","year":"2010","journal-title":"Nucleic Acids Res."},{"key":"2026090207225785900_cit55","doi-asserted-by":"crossref","first-page":"2039","DOI":"10.1007\/s00018-008-7554-z","article-title":"Evolution\nof the arginase fold and functional diversity","volume":"65","author":"Dowling","year":"2008","journal-title":"Cell. Mol. Life Sci."},{"key":"2026090207225785900_cit56","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1021\/bi801911v","article-title":"Probing\nthe specificity determinants of amino acid recognition by arginase","volume":"48","author":"Shishova","year":"2009","journal-title":"Biochemistry"},{"key":"2026090207225785900_cit57","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/j.ab.2006.07.027","article-title":"Thermofluor-based\nhigh-throughput stability optimization of proteins for structural\nstudies","volume":"357","author":"Ericsson","year":"2006","journal-title":"Anal. Biochem."},{"key":"2026090207225785900_cit58","doi-asserted-by":"crossref","first-page":"13974","DOI":"10.1021\/bi801648r","article-title":"Functional assignment\nof solute-binding\nproteins of ABC transporters using a fluorescence-based thermal shift\nassay","volume":"47","author":"Giuliani","year":"2008","journal-title":"Biochemistry"},{"key":"2026090207225785900_cit59","doi-asserted-by":"crossref","first-page":"12984","DOI":"10.1073\/pnas.1304045110","article-title":"Integrated\nbiophysical\napproach to fragment screening and validation for fragment-based lead\ndiscovery","volume":"110","author":"Silvestre","year":"2013","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"2026090207225785900_cit60","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1126\/science.1112642","article-title":"The genome of the\nAfrican trypanosome Trypanosoma brucei","volume":"309","author":"Berriman","year":"2005","journal-title":"Science"},{"key":"2026090207225785900_cit61","doi-asserted-by":"crossref","first-page":"D457","DOI":"10.1093\/nar\/gkp851","article-title":"TriTrypDB: A functional genomic resource for the Trypanosomatidae","volume":"38","author":"Aslett","year":"2010","journal-title":"Nucleic Acids Res."},{"key":"2026090207225785900_cit62","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1016\/j.str.2012.03.009","article-title":"Recognizing\nprotein-ligand binding sites by global structural alignment\nand local geometry refinement","volume":"20","author":"Roy","year":"2012","journal-title":"Structure"},{"key":"2026090207225785900_cit63","doi-asserted-by":"crossref","first-page":"8721","DOI":"10.1016\/S0021-9258(18)47475-3","article-title":"Cloning\nand sequencing of the ornithine\ndecarboxylase gene from Trypanosoma brucei","volume":"262","author":"Phillips","year":"1987","journal-title":"J. Biol. Chem."},{"key":"2026090207225785900_cit64","doi-asserted-by":"crossref","first-page":"17933","DOI":"10.1016\/S0021-9258(19)81306-6","article-title":"Trypanosoma brucei ornithine decarboxylase: Enzyme purification, characterization,\nand expression in Escherichia coli","volume":"263","author":"Phillips","year":"1988","journal-title":"J. Biol. Chem."},{"key":"2026090207225785900_cit65","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1093\/jn\/115.4.509","article-title":"Conversion\nof glutamate to ornithine and proline: Pyrroline-5-carboxylate, a\npossible modulator of arginine requirements","volume":"115","author":"Jones","year":"1985","journal-title":"J. Nutr."},{"key":"2026090207225785900_cit66","doi-asserted-by":"crossref","first-page":"14100","DOI":"10.1021\/ja054081g","article-title":"argE-Encoded N-acetyl-l-ornithine deacetylase\nfrom Escherichia coli contains a dinuclear metalloactive\nsite","volume":"127","author":"McGregor","year":"2005","journal-title":"J. Am. Chem. Soc."},{"key":"2026090207225785900_cit67","doi-asserted-by":"crossref","first-page":"2577","DOI":"10.1002\/bip.360221211","article-title":"Dictionary of protein\nsecondary structure: Pattern\nrecognition of hydrogen-bonded and geometrical features","volume":"22","author":"Kabsch","year":"1983","journal-title":"Biopolymers"},{"key":"2026090207225785900_cit68","doi-asserted-by":"crossref","first-page":"10037","DOI":"10.1073\/pnas.181342398","article-title":"Electrostatics\nof nanosystems: Application to microtubules and the ribosome","volume":"98","author":"Baker","year":"2001","journal-title":"Proc. Natl. Acad. Sci. U.S.A."}],"container-title":["Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/bi501366a","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/bichaw\/article-pdf\/54\/2\/458\/15709614\/bi501366a.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/bichaw\/article-pdf\/54\/2\/458\/15709614\/bi501366a.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T11:23:14Z","timestamp":1788348194000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/bichaw\/article\/54\/2\/458\/1478029\/Crystal-Structure-of-an-Arginase-like-Protein-from"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,12,23]]},"references-count":68,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2014,12,23]]},"published-print":{"date-parts":[[2015,1,20]]}},"URL":"https:\/\/doi.org\/10.1021\/bi501366a","relation":{},"ISSN":["0006-2960","1520-4995"],"issn-type":[{"value":"0006-2960","type":"print"},{"value":"1520-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,12,23]]}}}