{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:09:07Z","timestamp":1791418147769,"version":"4.3.3"},"reference-count":66,"publisher":"American Chemical Society (ACS)","issue":"21","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biochemistry"],"published-print":{"date-parts":[[2010,6,1]]},"DOI":"10.1021\/bi902201y","type":"journal-article","created":{"date-parts":[[2010,4,28]],"date-time":"2010-04-28T14:10:41Z","timestamp":1272463841000},"page":"4483-4493","source":"Crossref","is-referenced-by-count":39,"title":["Quantitative Analysis of VirB8\u2212VirB9\u2212VirB10 Interactions Provides a Dynamic Model of Type IV Secretion System Core Complex Assembly"],"prefix":"10.1021","volume":"49","author":[{"given":"Durga","family":"Sivanesan","sequence":"first","affiliation":[{"name":"Department of Biology, McMaster University, 1280 Main Street West, Hamilton, ON L8S 4K1, Canada"},{"name":"D\u00e9partement de biochimie, Universit\u00e9 de Montr\u00e9al, C.P. 6128, Succ. Centre-ville, Montr\u00e9al, QC H3C 3J7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark A.","family":"Hancock","sequence":"additional","affiliation":[{"name":"Sheldon Biotechnology Centre, McGill University, 3773 University Street, Montr\u00e9al, QC H3A 2B4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Mar\u00eda","family":"Villamil Giraldo","sequence":"additional","affiliation":[{"name":"D\u00e9partement de biochimie, Universit\u00e9 de Montr\u00e9al, C.P. 6128, Succ. Centre-ville, Montr\u00e9al, QC H3C 3J7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Baron","sequence":"additional","affiliation":[{"name":"Department of Biology, McMaster University, 1280 Main Street West, Hamilton, ON L8S 4K1, Canada"},{"name":"D\u00e9partement de biochimie, Universit\u00e9 de Montr\u00e9al, C.P. 6128, Succ. Centre-ville, Montr\u00e9al, QC H3C 3J7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2010,5,6]]},"reference":[{"key":"ref1\/cit1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1365-2958.2004.04168.x","volume":"53","author":"Llosa M.","year":"2004","journal-title":"Mol. Microbiol."},{"key":"ref2\/cit2","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.bbamcr.2004.02.013","volume":"1694","author":"Christie P. J.","year":"2004","journal-title":"Biochim. Biophys. Acta"},{"key":"ref3\/cit3","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1038\/nrmicro753","volume":"1","author":"Cascales E.","year":"2003","journal-title":"Nat. Rev. Microbiol."},{"key":"ref4\/cit4","doi-asserted-by":"crossref","first-page":"1359","DOI":"10.1046\/j.1365-2958.2002.02816.x","volume":"43","author":"Baron C.","year":"2002","journal-title":"Mol. Microbiol."},{"key":"ref5\/cit5","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.femsle.2005.09.030","volume":"253","author":"Baron C.","year":"2005","journal-title":"FEMS Microbiol. Lett."},{"key":"ref6\/cit6","doi-asserted-by":"crossref","first-page":"1567","DOI":"10.1016\/S0960-9822(02)70773-2","volume":"6","author":"Baron C.","year":"1996","journal-title":"Curr. Biol."},{"key":"ref7\/cit7","doi-asserted-by":"crossref","first-page":"1210","DOI":"10.1046\/j.1365-2958.1999.01569.x","volume":"33","author":"O\u2019Callaghan D.","year":"1999","journal-title":"Mol. Microbiol."},{"key":"ref8\/cit8","doi-asserted-by":"crossref","first-page":"1378","DOI":"10.1111\/j.1365-2958.2008.06487.x","volume":"70","author":"de Jong M. F.","year":"2008","journal-title":"Mol. Microbiol."},{"key":"ref9\/cit9","doi-asserted-by":"crossref","first-page":"1851","DOI":"10.1111\/j.1462-5822.2007.00922.x","volume":"9","author":"Roux C. M.","year":"2007","journal-title":"Cell. Microbiol."},{"key":"ref10\/cit10","doi-asserted-by":"crossref","first-page":"1210","DOI":"10.1046\/j.1365-2958.1999.01569.x","volume":"33","author":"O\u2019Callaghan D.","year":"1999","journal-title":"Mol. Microbiol."},{"key":"ref11\/cit11","doi-asserted-by":"crossref","first-page":"20019","DOI":"10.1073\/pnas.0701738104","volume":"104","author":"Guo M.","year":"2007","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"ref12\/cit12","doi-asserted-by":"crossref","first-page":"1699","DOI":"10.1046\/j.1365-2958.2003.03669.x","volume":"49","author":"Atmakuri K.","year":"2003","journal-title":"Mol. Microbiol."},{"key":"ref13\/cit13","doi-asserted-by":"crossref","first-page":"5143","DOI":"10.1128\/IAI.72.9.5143-5149.2004","volume":"72","author":"den Hartigh A. B.","year":"2004","journal-title":"Infect. Immun."},{"key":"ref14\/cit14","doi-asserted-by":"crossref","first-page":"4427","DOI":"10.1128\/JB.00406-08","volume":"190","author":"den Hartigh A. B.","year":"2008","journal-title":"J. Bacteriol."},{"key":"ref15\/cit15","doi-asserted-by":"crossref","first-page":"6048","DOI":"10.1128\/IAI.73.9.6048-6054.2005","volume":"73","author":"Sun Y. H.","year":"2005","journal-title":"Infect. Immun."},{"key":"ref16\/cit16","doi-asserted-by":"crossref","first-page":"3455","DOI":"10.1099\/mic.0.28141-0","volume":"151","author":"Zahrl D.","year":"2005","journal-title":"Microbiology"},{"key":"ref17\/cit17","doi-asserted-by":"crossref","first-page":"6551","DOI":"10.1128\/JB.00480-07","volume":"189","author":"Zupan J.","year":"2007","journal-title":"J. Bacteriol."},{"key":"ref18\/cit18","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1126\/science.1166101","volume":"323","author":"Fronzes R.","year":"2009","journal-title":"Science"},{"key":"ref19\/cit19","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1146\/annurev.micro.58.030603.123630","volume":"59","author":"Christie P. J.","year":"2005","journal-title":"Annu. Rev. Microbiol."},{"key":"ref20\/cit20","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1128\/IAI.74.1.108-117.2006","volume":"74","author":"Carle A.","year":"2006","journal-title":"Infect. Immun."},{"key":"ref21\/cit21","doi-asserted-by":"crossref","first-page":"11405","DOI":"10.1073\/pnas.172390699","volume":"99","author":"Krall L.","year":"2002","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"ref22\/cit22","doi-asserted-by":"crossref","first-page":"11493","DOI":"10.1073\/pnas.172390299","volume":"99","author":"Ward D.","year":"2002","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"ref23\/cit23","doi-asserted-by":"crossref","first-page":"26349","DOI":"10.1074\/jbc.M502347200","volume":"280","author":"Yuan Q.","year":"2005","journal-title":"J. Biol. Chem."},{"key":"ref24\/cit24","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1128\/JB.182.3.758-763.2000","volume":"182","author":"Das A.","year":"2000","journal-title":"J. Bacteriol."},{"key":"ref25\/cit25","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1038\/sj.embor.7400722","volume":"7","author":"Vetsch M.","year":"2006","journal-title":"EMBO Rep."},{"key":"ref26\/cit26","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1016\/j.jmb.2007.01.034","volume":"367","author":"Vitagliano L.","year":"2007","journal-title":"J. Mol. Biol."},{"key":"ref27\/cit27","first-page":"29","volume":"29","author":"Chandran V.","year":"2009","journal-title":"Nature"},{"key":"ref28\/cit28","doi-asserted-by":"crossref","first-page":"3404","DOI":"10.1128\/jb.179.11.3404-3409.1997","volume":"179","author":"Das A.","year":"1997","journal-title":"J. Bacteriol."},{"key":"ref29\/cit29","doi-asserted-by":"crossref","first-page":"8889","DOI":"10.1073\/pnas.93.17.8889","volume":"93","author":"Anderson L. B.","year":"1996","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"ref30\/cit30","doi-asserted-by":"crossref","first-page":"3156","DOI":"10.1128\/jb.178.11.3156-3167.1996","volume":"178","author":"Fernandez D.","year":"1996","journal-title":"J. Bacteriol."},{"key":"ref31\/cit31","doi-asserted-by":"crossref","first-page":"3168","DOI":"10.1128\/jb.178.11.3168-3176.1996","volume":"178","author":"Fernandez D.","year":"1996","journal-title":"J. Bacteriol."},{"key":"ref32\/cit32","doi-asserted-by":"crossref","first-page":"2867","DOI":"10.1128\/JB.185.9.2867-2878.2003","volume":"185","author":"Jakubowski S. J.","year":"2003","journal-title":"J. Bacteriol."},{"key":"ref33\/cit33","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1046\/j.1365-2958.1998.00688.x","volume":"27","author":"Das A.","year":"1998","journal-title":"Mol. Microbiol."},{"key":"ref34\/cit34","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1006\/plas.1994.1057","volume":"32","author":"Beijersbergen A.","year":"1994","journal-title":"Plasmid"},{"key":"ref35\/cit35","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1128\/jb.179.4.1211-1218.1997","volume":"179","author":"Baron C.","year":"1997","journal-title":"J. Bacteriol."},{"key":"ref36\/cit36","doi-asserted-by":"crossref","first-page":"3259","DOI":"10.1128\/JB.185.11.3259-3269.2003","volume":"185","author":"Liu Z.","year":"2003","journal-title":"J. Bacteriol."},{"key":"ref37\/cit37","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1046\/j.1365-2958.2000.01876.x","volume":"36","author":"Kumar R. B.","year":"2000","journal-title":"Mol. Microbiol."},{"key":"ref38\/cit38","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1128\/jb.179.1.78-89.1997","volume":"179","author":"Beaupr\u00e9 C. E.","year":"1997","journal-title":"J. Bacteriol."},{"key":"ref39\/cit39","doi-asserted-by":"crossref","first-page":"17228","DOI":"10.1073\/pnas.0405843101","volume":"101","author":"Cascales E.","year":"2004","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"ref40\/cit40","doi-asserted-by":"crossref","first-page":"3646","DOI":"10.1128\/jb.176.12.3646-3660.1994","volume":"176","author":"Berger B. R.","year":"1994","journal-title":"J. Bacteriol."},{"key":"ref41\/cit41","doi-asserted-by":"crossref","first-page":"7252","DOI":"10.1073\/pnas.0600862103","volume":"103","author":"Paschos A.","year":"2006","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"ref42\/cit42","doi-asserted-by":"crossref","first-page":"5200","DOI":"10.1128\/jb.172.9.5200-5210.1990","volume":"172","author":"Ward J. E.","year":"1990","journal-title":"J. Bacteriol."},{"key":"ref43\/cit43","doi-asserted-by":"crossref","first-page":"3486","DOI":"10.1128\/JB.187.10.3486-3495.2005","volume":"187","author":"Jakubowski S. J.","year":"2005","journal-title":"J. Bacteriol."},{"key":"ref44\/cit44","doi-asserted-by":"crossref","first-page":"3636","DOI":"10.1128\/JB.183.12.3636-3641.2001","volume":"183","author":"Kumar R. B.","year":"2001","journal-title":"J. Bacteriol."},{"key":"ref45\/cit45","doi-asserted-by":"crossref","first-page":"11498","DOI":"10.1073\/pnas.0505290102","volume":"102","author":"Judd P. K.","year":"2005","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"ref46\/cit46","doi-asserted-by":"crossref","first-page":"5572","DOI":"10.1128\/JB.184.20.5572-5582.2002","volume":"184","author":"Ding Z.","year":"2002","journal-title":"J. Bacteriol."},{"key":"ref47\/cit47","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Maniatis T. A.","year":"1982"},{"key":"ref48\/cit48","unstructured":"Paschos, A., den Hartig, A., Coincon, M., Sivanesan, D., Sygusch, J., Tsolis, R. M., and Baron, C. (2010) Anin vivohigh-throughput screening approach targeting the type IV secretion system component VirB8 identified inhibitors of intracellular proliferation ofBrucella.PLoS Pathog., submitted."},{"key":"ref49\/cit49","doi-asserted-by":"crossref","first-page":"11768","DOI":"10.1021\/bi035234k","volume":"42","author":"Taneva S.","year":"2003","journal-title":"Biochemistry"},{"key":"ref50\/cit50","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1006\/abio.2000.4880","volume":"287","author":"Sreerama N.","year":"2000","journal-title":"Anal. Biochem."},{"key":"ref51\/cit51","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1002\/(SICI)1099-1352(199909\/10)12:5<279::AID-JMR473>3.0.CO;2-3","volume":"12","author":"Myszka D. G.","year":"1999","journal-title":"J. Mol. Recognit."},{"key":"ref52\/cit52","doi-asserted-by":"crossref","first-page":"5752","DOI":"10.1073\/pnas.95.10.5752","volume":"95","author":"Karimova G.","year":"1998","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"ref53\/cit53","doi-asserted-by":"crossref","first-page":"2985","DOI":"10.1128\/JB.01426-08","volume":"191","author":"Bourg G.","year":"2009","journal-title":"J. Bacteriol."},{"key":"ref54\/cit54","doi-asserted-by":"crossref","first-page":"7156","DOI":"10.1128\/AEM.01397-06","volume":"72","author":"Cowie A.","year":"2006","journal-title":"Appl. Environ. Microbiol."},{"key":"ref55\/cit55","doi-asserted-by":"crossref","first-page":"4596","DOI":"10.1073\/pnas.0408927102","volume":"102","author":"Terradot L.","year":"2005","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"ref56\/cit56","doi-asserted-by":"crossref","first-page":"2582","DOI":"10.1073\/pnas.0511216103","volume":"103","author":"Bailey S.","year":"2006","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"ref57\/cit57","doi-asserted-by":"crossref","first-page":"36293","DOI":"10.1074\/jbc.M508089200","volume":"280","author":"Quinaud M.","year":"2005","journal-title":"J. Biol. Chem."},{"key":"ref58\/cit58","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1186\/1471-2180-3-21","volume":"3","author":"Nanao M.","year":"2003","journal-title":"BMC Microbiol."},{"key":"ref59\/cit59","doi-asserted-by":"crossref","first-page":"2580","DOI":"10.1016\/S0006-3495(02)75600-2","volume":"82","author":"Greenfield N. J.","year":"2002","journal-title":"Biophys. J."},{"key":"ref60\/cit60","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1007\/s12195-008-0035-5","volume":"1","author":"De Crescenzo G.","year":"2008","journal-title":"Cell. Mol. Bioeng."},{"key":"ref61\/cit61","doi-asserted-by":"crossref","first-page":"3469","DOI":"10.1099\/mic.0.28326-0","volume":"151","author":"H\u00f6ppner C.","year":"2005","journal-title":"Microbiology"},{"key":"ref62\/cit62","doi-asserted-by":"crossref","first-page":"35413","DOI":"10.1074\/jbc.M607611200","volume":"281","author":"Carter D. M.","year":"2006","journal-title":"J. Biol. Chem."},{"key":"ref63\/cit63","doi-asserted-by":"crossref","first-page":"3505","DOI":"10.1099\/mic.0.28410-0","volume":"151","author":"de Paz H. D.","year":"2005","journal-title":"Microbiology"},{"key":"ref64\/cit64","doi-asserted-by":"crossref","first-page":"1673","DOI":"10.1073\/pnas.0609535104","volume":"104","author":"Bayliss R.","year":"2007","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"ref65\/cit65","doi-asserted-by":"crossref","first-page":"5835","DOI":"10.1128\/jb.179.18.5835-5842.1997","volume":"179","author":"Zhou X. R.","year":"1997","journal-title":"J. Bacteriol."},{"key":"ref66\/cit66","doi-asserted-by":"crossref","first-page":"6597","DOI":"10.1128\/JB.180.24.6597-6606.1998","volume":"180","author":"Banta L. M.","year":"1998","journal-title":"J. Bacteriol."}],"container-title":["Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/bi902201y","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,3,24]],"date-time":"2023-03-24T00:44:45Z","timestamp":1679618685000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/doi\/10.1021\/bi902201y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,5,6]]},"references-count":66,"journal-issue":{"issue":"21","published-print":{"date-parts":[[2010,6,1]]}},"alternative-id":["10.1021\/bi902201y"],"URL":"https:\/\/doi.org\/10.1021\/bi902201y","relation":{},"ISSN":["0006-2960","1520-4995"],"issn-type":[{"value":"0006-2960","type":"print"},{"value":"1520-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,5,6]]}}}