{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T14:02:06Z","timestamp":1761487326497,"version":"build-2065373602"},"reference-count":22,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2008,9,5]],"date-time":"2008-09-05T00:00:00Z","timestamp":1220572800000},"content-version":"vor","delay-in-days":5483,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Progress"],"published-print":{"date-parts":[[1993,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The effects of varying the levels of cell disruption achieved by high\u2010pressure homogenization upon subsequent centrifugal separation of cell debris have been defined by measuring sedimentation velocity distributions of the cell debris. Using this data together with particle size distributions, a computer\u2010based model of a disk stack centrifuge has been used to predict the efficiency of centrifugal separation. These predictions have been compared with both laboratory scale and pilot plant scale experimental results. A similar sedimentation velocity technique has been used to characterize the properties of the floes formed by addition of poly (ethylene imine) to a borax\u2010clarified homogenate supernatant. The variations of floe settling properties with respect to polymer concentration are defined.<\/jats:p>","DOI":"10.1021\/bp00023a004","type":"journal-article","created":{"date-parts":[[2005,3,7]],"date-time":"2005-03-07T21:48:22Z","timestamp":1110232102000},"page":"462-467","source":"Crossref","is-referenced-by-count":40,"title":["A Study of Process Interactions between Cell Disruption and Debris Clarification Stages in the Recovery of Yeast Intracellular Products"],"prefix":"10.1002","volume":"9","author":[{"given":"Andrew I.","family":"Clarkson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick","family":"Lefevre","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nigel J.","family":"Titchener\u2010Hooker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,9,5]]},"reference":[{"key":"e_1_2_1_2_1","unstructured":"Alsaffar L.;Newsome P.;Titchener\u2010Hooker N. J.Purification of Alcohol Dehydrogenase (ADH) from Bakers' Yeast\u2014Process Interactions During Fractional Precipitation.Inst. Chem. Eng. Res. Event1993132\u2013134."},{"key":"e_1_2_1_3_1","unstructured":"Ambler C. M.The Evaluation of Centrifuge Performance.Chem. Eng. Prog.1952(March) 150\u2013158."},{"key":"e_1_2_1_4_1","unstructured":"Bell D. J.;Brunner K.\u2010H.A Method for the Evaluation of Floc Break\u2010Up in Centrifuges.Filtr. Sep.1983(July\/Aug) 274\u2013301."},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260360410"},{"key":"e_1_2_1_6_1","first-page":"127","article-title":"Occurrence and Biosynthesis of Very Long Fatty\u2010Acids and Alkanes in Plasmalemma Enriched Fractions from Saccaromyces cerevisiae","volume":"30","author":"Blanchardie P.","year":"1977","journal-title":"Biol. Cell."},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0141-0229(88)90015-4"},{"key":"e_1_2_1_8_1","unstructured":"Buscall R.;Stewart R. F.;Sutton D.Structure in Colloidal Suspensions and Effects on Solid\u2010Liquid Separation.Filtr. Sep.1984(May\/Jun) 183\u2013188."},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0098-1354(09)80052-5"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/bp00004a009"},{"key":"e_1_2_1_11_1","unstructured":"DCP\u20101000 Particle Sizer Manual Brookhaven Instruments Corporation U.S.A."},{"key":"e_1_2_1_12_1","first-page":"142","article-title":"Release of Protein from Baker's Yeast (Saccaromyces cerevisiae) by Disruption in an Industrial Homogeniser","volume":"49","author":"Hetherington P. J.","year":"1971","journal-title":"Trans. Inst. Chem. Eng."},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260200202"},{"key":"e_1_2_1_14_1","unstructured":"Mannweiler K.The Recovery of Biological Particles in High\u2010Speed Continuous Centrifuges with Special Reference to Feed\u2010Zone Break\u2010Up Effects. Ph.D. Thesis University of London London U.K. 1990."},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00369259"},{"key":"e_1_2_1_16_1","unstructured":"Mannweiler K.;Titchener\u2010Hooker N. J.;Hoare M.Biochemical Engineering Improvements in the Centrifugal Recovery of Biological Particles.Inst. Chem. Eng.\u2010Adv. Biochem. Eng.1989105\u2013117."},{"issue":"1","key":"e_1_2_1_17_1","first-page":"527","article-title":"Selective Flocculation of Nucleic Acids, Lipids, and Colloidal Particles from a Cell Homogenate by Polyethyleneimine, and its Scale\u2010Up","volume":"12","author":"Milburn P.","year":"1989","journal-title":"Enzyme Microb. Technol."},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260230208"},{"key":"e_1_2_1_19_1","unstructured":"Obeng E. D. A.Concentration of Protein Solutions by Evaporation. Ph.D. Thesis University of London London U.K. 1983."},{"key":"e_1_2_1_20_1","first-page":"35","article-title":"Sedimentation and Fluidisation: Part 1","volume":"32","author":"Richardson J. F.","year":"1954","journal-title":"Trans. Inst. Chem. Eng."},{"key":"e_1_2_1_21_1","unstructured":"Salt D. E.;Bentham A. C.;Hay S.;Idris A.;Hoare M.;Dunnill P.Mechanism of Flocculation of aSaccaromyces cerevisiaeCellular Homogenate using Polyethyleneimine.1993amanuscript in preparation."},{"key":"e_1_2_1_22_1","unstructured":"Salt D. E.;Hay S.;Idris A.;Hoare M.;Dunnill P.Selective Flocculation of a Range of Cellular Contaminants from Soluble Proteins Using Polyethyleneimine: An Extended Study of Organisms and Polymers.1993bmanuscript in preparation."},{"key":"e_1_2_1_23_1","unstructured":"Siddiqi S. F.;Clarkson A. I.;Keshavarz\u2010Moore E.;Titchener\u2010Hooker N. J.Modelling of Process Interactions in Biochemical Processes.Inst. Chem. Eng. Res. Event1991155\u2013158."}],"container-title":["Biotechnology Progress"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1021%2Fbp00023a004","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/pdf\/10.1021\/bp00023a004","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T19:29:05Z","timestamp":1760038145000},"score":1,"resource":{"primary":{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/10.1021\/bp00023a004"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,9]]},"references-count":22,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1993,9]]}},"alternative-id":["10.1021\/bp00023a004"],"URL":"https:\/\/doi.org\/10.1021\/bp00023a004","archive":["Portico"],"relation":{},"ISSN":["8756-7938","1520-6033"],"issn-type":[{"type":"print","value":"8756-7938"},{"type":"electronic","value":"1520-6033"}],"subject":[],"published":{"date-parts":[[1993,9]]}}}