{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T01:40:37Z","timestamp":1790732437497,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2008,9,5]],"date-time":"2008-09-05T00:00:00Z","timestamp":1220572800000},"content-version":"vor","delay-in-days":2074,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Progress"],"published-print":{"date-parts":[[2003,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Loofa sponge was investigated as a three\u2010dimensional scaffold for stationary and perfusion culture of human hepatoblastoma cell line C3A\/HepG2. In stationary culture, C3A\/HepG2 cells in loofa cubes showed higher \u03b1\u2010fetoprotein and albumin secretion rates than those in polyurethane foam (PU). To use loofa cylinders in a packed\u2010bed reactor, immobilization of C3A\/HepG2 cells by recirculating medium at 26 mL\/min (superficial velocity = 51.7 cm\/min) resulted in a cell loading density of 5.15 \u00d7 10\n                    <jats:sup>7<\/jats:sup>\n                    cells\/cm\n                    <jats:sup>3<\/jats:sup>\n                    \u2010loofa. This cell loading density is higher than values reported in the literature for packed\u2010bed reactor intended for bioartificial liver. During 9 days of perfusion culture in the reactor, immobilized C3A\/HepG2 showed steady synthesis of albumin with an average synthesis rate at 42.2 \u03bcg\/10\n                    <jats:sup>6<\/jats:sup>\n                    cells\/day. These experimental results and observations by SEM suggested that loofa sponge is a suitable scaffold for high\u2010density culture of human hepatocyte cell line and the immobilized cells could express high levels of liver\u2010specific functions.\n                  <\/jats:p>","DOI":"10.1021\/bp025720j","type":"journal-article","created":{"date-parts":[[2003,4,4]],"date-time":"2003-04-04T01:42:07Z","timestamp":1049420527000},"page":"522-527","source":"Crossref","is-referenced-by-count":36,"title":["Loofa Sponge as a Scaffold for the Culture of Human Hepatocyte Cell Line"],"prefix":"10.1002","volume":"19","author":[{"given":"Jyh\u2010Ping","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng\u2010Chun","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brend Ray\u2010Sea","family":"Hsu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shin\u2010Huei","family":"Fu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hwai\u2010Shen","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,9,5]]},"reference":[{"key":"e_1_2_5_2_2","first-page":"171","article-title":"A Study of the mechanism of gastrulation. 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