{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T03:12:10Z","timestamp":1791515530223,"version":"4.3.4"},"reference-count":53,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2008,9,5]],"date-time":"2008-09-05T00:00:00Z","timestamp":1220572800000},"content-version":"vor","delay-in-days":2074,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Progress"],"published-print":{"date-parts":[[2003,1]]},"abstract":"Abstract<\/jats:title>\n \n The dihydroxyacetone (\n dha<\/jats:italic>\n ) regulon of bacteria encodes genes for the anaerobic metabolism of glycerol. In this work, genomic data are used to analyze and compare the\n dha<\/jats:italic>\n regulon and related genes in different organisms in silico with respect to gene organization, sequence similarity, and possible functions. Database searches showed that among the organisms, the genomes of which have been sequenced so far, only two, i.e.,\n Klebsiella pneumoniae<\/jats:italic>\n MGH 78578 and\n Clostridium perfringens<\/jats:italic>\n contain a complete\n dha<\/jats:italic>\n regulon bearing all known enzymes. The components and their organization in the\n dha<\/jats:italic>\n regulon of these two organisms differ considerably from each other and also from the previously partially sequenced\n dha<\/jats:italic>\n regulons in\n Citrobacter freundii<\/jats:italic>\n ,\n Clostridium pasteurianum<\/jats:italic>\n , and\n Clostridium<\/jats:italic>\n butyricum<\/jats:italic>\n . Unlike all of the other organisms, genes for the oxidative and reductive pathways of anaerobic glycerol metabolism in\n C.<\/jats:italic>\n perfringens<\/jats:italic>\n are located in two separate organization units on the chromosome. Comparisons of deduced protein sequences of genes with similar functions showed that the\n dha<\/jats:italic>\n regulon components in\n K.<\/jats:italic>\n pneumoniae<\/jats:italic>\n and\n C. freundii<\/jats:italic>\n have high similarities (80\u201395%) but lower similarities to those of the\n Clostridium<\/jats:italic>\n species (30\u201380%). Interestingly, the protein sequence similarities among the\n dha<\/jats:italic>\n genes of the\n Clostridium<\/jats:italic>\n species are in many cases even lower than those between the\n Clostridium<\/jats:italic>\n species and\n K. pneumoniae<\/jats:italic>\n or\n C. freundii<\/jats:italic>\n , suggesting two different types of\n dha<\/jats:italic>\n regulon in the\n Clostridium<\/jats:italic>\n species studied. The in silico reconstruction and comparison of\n dha<\/jats:italic>\n regulons revealed several new genes in the microorganisms studied. In particular, a novel\n dha<\/jats:italic>\n kinase that is phosphoenolpyruvate\u2010dependent is identified and experimentally confirmed for\n K. pneumoniae<\/jats:italic>\n in addition to the known ATP\u2010dependent\n dha<\/jats:italic>\n kinase. This finding gives new insights into the regulation of glycerol metabolism in\n K.<\/jats:italic>\n pneumoniae<\/jats:italic>\n and explains some hitherto not well understood experimental observations.\n <\/jats:p>","DOI":"10.1021\/bp025739m","type":"journal-article","created":{"date-parts":[[2003,4,4]],"date-time":"2003-04-04T01:42:07Z","timestamp":1049420527000},"page":"263-272","source":"Crossref","is-referenced-by-count":79,"title":["Comparative Genomic Analysis of\n dha<\/i>\n Regulon and Related Genes for Anaerobic Glycerol Metabolism in Bacteria"],"prefix":"10.1002","volume":"19","author":[{"given":"Jibin","family":"Sun","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joop","family":"van den Heuvel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Soucaille","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinbo","family":"Qu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"An\u2010Ping","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,9,5]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-765X.1996.tb01168.x"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0290(19980905)59:5<544::AID-BIT3>3.0.CO;2-A"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.1.37"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.22.5932-5946.1998"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002530051523"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1078\/07232020260517616"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.15.4129-4136.2000"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/bp9701325"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4838(00)00234-X"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-67-1-33"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.1998.tb00387.x"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.8.2151-2156.1995"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.15.4392-4401.1995"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002030050585"},{"key":"e_1_2_6_17_2","unstructured":"Dunn\u2010Coleman A.;Diaz U.;Torres M.;Chase M. 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