{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T07:31:40Z","timestamp":1762673500582,"version":"build-2065373602"},"reference-count":43,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2008,9,5]],"date-time":"2008-09-05T00:00:00Z","timestamp":1220572800000},"content-version":"vor","delay-in-days":1343,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Progress"],"published-print":{"date-parts":[[2005,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Fermentation efficiency and nutrient costs are both significant factors in process economics for the microbial conversion of cellulosic biomass to commodity chemicals such as ethanol. In this study, we have developed a more industrial medium (OUM1) composed of 0.5% corn steep liquor (dry weight basis) supplemented with mineral salts (0.2%), urea (0.06%), and glucose (9%). Although the growth of strain P2 was vigorous in this medium, approximately 14% of substrate carbon was diverted into 2,3\u2010butanediol and acetoin under the low pH conditions needed for optimal cellulase activity during simultaneous saccharification. Deleting the central region of the <jats:italic>budAB<\/jats:italic> genes encoding \u03b1\u2010acetolactate synthase and \u03b1\u2010acetolactate decarboxylase eliminated the butanediol and acetoin coproducts and increased ethanol yields by 12%. In OUM1 medium at pH 5.2, strain BW21 produced over 4% ethanol in 48 h (0.47 g ethanol per g glucose). Average productivity (48 h), ethanol titer, and ethanol yield for BW21 in OUM1 medium (pH 5.2) exceeded that of the parent (strain P2) in rich laboratory medium (Luria broth).<\/jats:p>","DOI":"10.1021\/bp050100e","type":"journal-article","created":{"date-parts":[[2005,10,7]],"date-time":"2005-10-07T00:20:03Z","timestamp":1128644403000},"page":"1366-1372","source":"Crossref","is-referenced-by-count":44,"title":["Development of Industrial\u2010Medium\u2010Required Elimination of the 2,3\u2010Butanediol Fermentation Pathway To Maintain Ethanol Yield in an Ethanologenic Strain of <i>Klebsiella<\/i><i>oxytoca<\/i>"],"prefix":"10.1002","volume":"21","author":[{"given":"Brent E.","family":"Wood","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. P.","family":"Yomano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. 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