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The cell could tolerate high ketone <jats:bold>1<\/jats:bold> concentration of 233.8 mmol\/L (i.e., 36 g\/L) with considerable reduction activity in this method. The product <jats:bold>2<\/jats:bold> concentration achieved 38.9 and 58.4 mmol\/L with cells of 40 and 60 g<jats:sub>DCW<\/jats:sub> (dry cell weight)'L, respectively, in 24 h. The optimum reaction time, the effect of substrate concentration, cosubstrate type and concentration, and cell concentration in the reaction were investigated in this paper.<\/jats:p>","DOI":"10.1021\/bp0600583","type":"journal-article","created":{"date-parts":[[2006,7,13]],"date-time":"2006-07-13T04:00:24Z","timestamp":1152763224000},"page":"1301-1304","source":"Crossref","is-referenced-by-count":19,"title":["Asymmetric Reduction of <i>o<\/i>\u2010Chloroacetophenone with <i>Candida pseudotropicalis<\/i>104"],"prefix":"10.1002","volume":"22","author":[{"given":"Qing","family":"Xie","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianping","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gang","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lirong","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,9,5]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tetasy.2005.02.030"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-4039(98)01029-6"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tetasy.2004.12.010"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/jo9812779"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1381-1177(98)00111-8"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002530051536"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tet.2003.11.066"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.enzmictec.2005.07.007"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcatb.2005.04.012"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.enzmictec.2005.02.018"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.60.1826"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1021\/bp0342046"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-4020(98)00796-0"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1389-1723(00)88954-9"},{"key":"e_1_2_6_16_2","doi-asserted-by":"crossref","unstructured":"Bucciarelli M.;Forni A.;Moretti I.;Torre G.Asymmetric reduction of trifluoromethyl and methyl ketones by yeast; an improved method.Synthese1983 897\u2013899.","DOI":"10.1055\/s-1983-30555"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tetasy.2004.09.021"},{"issue":"8","key":"e_1_2_6_18_2","doi-asserted-by":"crossref","first-page":"2374","DOI":"10.1128\/aem.56.8.2374-2377.1990","article-title":"Stereoselective reduction of ethyl 4\u2010chloro\u20103\u2010oxobutanoate by a microbial aldehyde reductase in an organic solvent\u2010water diphasic system.","volume":"56","author":"Shimizu S.","year":"1990","journal-title":"Appl. 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