{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T22:37:56Z","timestamp":1790894276685,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2008,9,5]],"date-time":"2008-09-05T00:00:00Z","timestamp":1220572800000},"content-version":"vor","delay-in-days":188,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Progress"],"published-print":{"date-parts":[[2008,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The selectivity of polyethyleneimine (PEI) in DNA precipitation during chemical extraction was investigated. Chemical extraction was used to recover two His\u2010tagged model proteins: gloshedobin, a thrombin\u2010like enzyme from snake venom, and IbpA, a molecular chaperone, which were expressed mainly in the form of inclusion bodies. High DNA removal efficiency (more than 90%) was achieved at various cell densities (with OD<jats:sub>600<\/jats:sub> ranging from 30 to 150) without affecting the solubility of host cell proteins. Compared to spermine\u2010induced precipitation method reported elsewhere, PEI provided a higher DNA precipitation efficiency at a significantly lower cost. Moreover, PEI obviated the use of EDTA, which has been reported to be essential for the chemical extraction methods, hence exhibiting dual roles in replacing cost\u2010prohibitive spermine and EDTA. The residual PEI in the post\u2010extraction mixture was efficiently counteracted by addition of Mg<jats:sup>2+<\/jats:sup>, allowing the streamlined application of the extraction broth to immobilized metal affinity chromatography. Taken together, the PEI\u2010mediated chemical extraction method provides a simpler and more economically viable processing route for the production of recombinant proteins whose expression is hampered by IB formation.<\/jats:p>","DOI":"10.1021\/bp070304q","type":"journal-article","created":{"date-parts":[[2008,1,24]],"date-time":"2008-01-24T06:00:54Z","timestamp":1201154454000},"page":"417-425","source":"Crossref","is-referenced-by-count":7,"title":["Polyethyleneimine\u2010Mediated Chemical Extraction of Cytoplasmic His\u2010Tagged Inclusion Body Proteins from <i>Escherichia coli<\/i>"],"prefix":"10.1002","volume":"24","author":[{"given":"Rui","family":"Nian","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihan","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Woo\u2010Seok","family":"Choe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,9,5]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"1107","article-title":"Selective precipitation of DNA by spermine during the chemical extraction of insoluble cytoplasmic protein. 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