{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T02:04:13Z","timestamp":1760061853305,"version":"build-2065373602"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2008,9,11]],"date-time":"2008-09-11T00:00:00Z","timestamp":1221091200000},"content-version":"vor","delay-in-days":4637,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Progress"],"published-print":{"date-parts":[[1996,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To investigate the possibility of luciferase\u2010dependent photoreduction of cytochrome P\u2010450's <jats:italic>in vivo<\/jats:italic>, <jats:italic>Vibrio harveyi<\/jats:italic>luciferase was coexpressed with the bacterial cytochrome P\u2010450cam in <jats:italic>Pseudomonas putida<\/jats:italic>. Luciferase expression was under the control of the Pm promoter from the meta\u2010cleavage TOL operon, incorporated into the chromosome by a mini Tn5\u2010mediated transposition. Cytochrome P\u2010450cam expression was controlled by the Ptac\u2010lac promoter on the broad host range vector pMMB206. Both proteins were expressed in <jats:italic>Pseudomonas<\/jats:italic><jats:italic>putida<\/jats:italic> strain MTCC 102 (PpW). This strain does <jats:italic>not<\/jats:italic> harbor the cam plasmid, which encodes the enzymes responsible for degradation of the terpene camphor. The metabolic activity of the resulting strain (PpW\u2010lux\u2010cam) toward model halogenated compounds was studied. In the absence of the natural cytochrome P\u2010450cam, electron transfer partners putidaredoxin (PR) and putidaredoxin reductase (Fp), and at low oxygen tension, the bacterial cells efficiently dehalogenate model chlorinated hydrocarbons in a light\u2010independent reaction. Hexachloroethane and pentachloroethane were metabolized to tetrachloroethylene and trichloroethylene, respectively, at rates comparable to that of strain PpG786, which carries the cam plasmid. Reductive dehalogenation required the expression of both luciferase and cytochrome P\u2010450 in the same cells. These results indicate that alternative electron transfer partners may be exploited for cytochrome P\u2010450\u2010dependent bioremediation strategies.<\/jats:p>","DOI":"10.1021\/bp9600389","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T01:30:15Z","timestamp":1027647015000},"page":"474-479","source":"Crossref","is-referenced-by-count":7,"title":["Luciferase\u2010Dependent, Cytochrome P\u2010450\u2010Catalyzed Dehalogenation in Genetically Engineered <i>Pseudomonas<\/i>"],"prefix":"10.1002","volume":"12","author":[{"given":"Rishi","family":"Shanker","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William M.","family":"Atkins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,9,11]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja00189a057"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(93)90344-3"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1139\/m91-134"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a130219"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.7.2121-2123.1994"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.1.7-14.1994"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.69.5.1273"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(78)52019-3"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(78)57016-X"},{"key":"e_1_2_6_11_1","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.1128\/aem.59.4.1194-1200.1993","article-title":"Enhanced Mineralization of Polychlorinated Biphenyls in Soils Inoculated with Chlorobenzoate Degrading Bacteria","volume":"59","author":"Hickey W. 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