{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:03:17Z","timestamp":1760140997935,"version":"build-2065373602"},"reference-count":22,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2008,9,5]],"date-time":"2008-09-05T00:00:00Z","timestamp":1220572800000},"content-version":"vor","delay-in-days":3900,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Progress"],"published-print":{"date-parts":[[1998,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In this study, we prepared bifunctional biopolymers for development of a novel liposomal immunosensing element. These biopolymers were produced such that a rat monoclonal antibody fragment Fab\u2032 was linked to a cardiac protein Troponin I (TnI) peptide by a cross\u2010linking reagent, <jats:italic>o<\/jats:italic>\u2010phenylenedimaleimide (<jats:italic>o<\/jats:italic>\u2010PDM) or <jats:italic>N<\/jats:italic>\u2010sucinimidyl 3\u2010(2\u2010pyridyldithio) propionate (SPDP) . The biopolymer formation yields were approximately 10% for Fab\u2010TnI<jats:sub>Mal<\/jats:sub> and 30% for Fab\u2010TnI<jats:sub>SPDP<\/jats:sub>. Molar ratios of Fab\u2032 to SPDP or <jats:italic>o<\/jats:italic>\u2010PDM and conjugated Fab\u2032 to TnI peptide and conjugation pH have considerable effects on the biopolymer yield. Purification of these biopolymers was achieved by employing size\u2010exclusion HPLC. These biopolymers can bind to receptor channels on one end, while the peptide end can be recognized by an anti\u2010TnI antibody serving as a protein linker to block the channels in the immunosensing element. Then reactions may be used where free analyte competes for cross\u2010linker binding sites whereby channels are rendered active. Characterization of purified biopolymers was performed using gel electrophoresis, ELISAs, and a BIAcore instrument. Furthermore, results of real\u2010time biospecific interaction experiments with use of the BIAcore show that competition binding reactions of free TnI peptide occurred in this new immunosensing design. The binding activities of these two biopolymers are slightly different.<\/jats:p>","DOI":"10.1021\/bp980012c","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T01:19:13Z","timestamp":1027646353000},"page":"310-317","source":"Crossref","is-referenced-by-count":2,"title":["Preparation and Characterization of Bifunctional Biopolymers for Receptor\u2010Based Liposomal Immunosensing"],"prefix":"10.1002","volume":"14","author":[{"given":"Yong\u2010Yi","family":"Yu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernard J.","family":"Van Wie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan R.","family":"Koch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David F.","family":"Moffett","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William C.","family":"Davis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,9,5]]},"reference":[{"key":"e_1_2_6_2_1","unstructured":"BIAtechnology Note 106 Pharmacia Biosensor AB Uppsala Sweden."},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(83)90177-1"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3925553"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(94)90042-6"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00316449"},{"key":"e_1_2_6_8_1","doi-asserted-by":"crossref","first-page":"2367","DOI":"10.4049\/jimmunol.139.7.2367","article-title":"Preparation and performance of bispecific F(ab\u2032\u03b3)2 antibody containing thioether\u2010linked Fab\u2032\u03b3 fragments","volume":"139","author":"Glennie M. 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Immunol."},{"key":"e_1_2_6_16_1","first-page":"1","volume-title":"Immunochemistry;","author":"Parham P.","year":"1986"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(81)90602-3"},{"key":"e_1_2_6_18_1","first-page":"191","volume-title":"Proceedings of Bispecific Antibodies and Targeted Cellular Cytotoxicity\u2010Second International Conference;","author":"Paulus H.","year":"1991"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01517179"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/314628a0"},{"volume-title":"Biochemistry;","year":"1990","author":"Voet D.","key":"e_1_2_6_21_1"},{"key":"e_1_2_6_22_1","unstructured":"Yu Y.Antibody modification and biospecific interaction analysis for receptor\u2010based liposomal immunosensing. Ph.D. dissertation Washington State University Pullman WA 1997."},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0956-5663(97)87059-5"}],"container-title":["Biotechnology Progress"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1021%2Fbp980012c","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/pdf\/10.1021\/bp980012c","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T23:51:19Z","timestamp":1760053879000},"score":1,"resource":{"primary":{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/10.1021\/bp980012c"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,1]]},"references-count":22,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1998,1]]}},"alternative-id":["10.1021\/bp980012c"],"URL":"https:\/\/doi.org\/10.1021\/bp980012c","archive":["Portico"],"relation":{},"ISSN":["8756-7938","1520-6033"],"issn-type":[{"type":"print","value":"8756-7938"},{"type":"electronic","value":"1520-6033"}],"subject":[],"published":{"date-parts":[[1998,1]]}}}