{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T21:34:15Z","timestamp":1768426455764,"version":"3.49.0"},"reference-count":11,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2008,9,4]],"date-time":"2008-09-04T00:00:00Z","timestamp":1220486400000},"content-version":"vor","delay-in-days":3534,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Progress"],"published-print":{"date-parts":[[1999,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Biological treatment of hazardous chemical wastes has potential as an effective, practical, and economically viable process in above the ground treatment systems that consist of both genetically engineered microorganisms (GEMs) and bioreactors with process control instruments to create ideal conditions for biodegradation. A strain of <jats:italic>Pseudomonas putida<\/jats:italic> coexpressing cytochrome P\u2010450cam and luciferase (lux) that provides both the reductive detoxification potential of the hemoprotein and a mechanism for its reduction in the absence of \u201cnormal\u201d P\u2010450 redox partners was evaluated for its ability to survive and remain metabolically competent under nutrient stress in soil slurry microcosms. More than 74% of the cells of engineered <jats:italic>Pseudomonas<\/jats:italic> were culturable after 7 days of multiple nutrient (C,N,P) starvation. The diagnostic luminescence and carbon monoxide\u2010difference spectra for the two engineered traits could be detected in a significant fraction of the surviving population. The GEM could be revived after repeated desiccation and starvation using Luria broth, benzoate, or citrate as nutrients. Soil slurries inoculated with the GEM transformed hexachloroethane (HCE) to tetrachloroethylene (tetraCE) 8\u201310\u2010fold faster than uninoculated slurries. The GEM also transformed the insecticide, \u03b3\u2010HCH (\u03b3\u20103,4,5,6\u2010hexachlorocyclhexene), to \u03b3\u20103,4,5,6\u2010tetrachlorocyclohexene (\u03b3tetraCH) in soil slurries under subatmospheric conditions. These results indicate that GEMs can be constructed with broad substrate range detoxification catalysts such as cytochrome P\u2010450 for remediation.<\/jats:p>","DOI":"10.1021\/bp990097x","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T01:32:33Z","timestamp":1027647153000},"page":"958-962","source":"Crossref","is-referenced-by-count":8,"title":["Stress Survival of a Genetically Engineered <i>Pseudomonas<\/i> in Soil Slurries: Cytochrome P\u2010450cam\u2010Catalyzed Dehalogenation of Chlorinated Hydrocarbons"],"prefix":"10.1002","volume":"15","author":[{"given":"Kavita","family":"Rattan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rishi","family":"Shanker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Purushottam","family":"Khanna","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William M.","family":"Atkins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,9,4]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0273-1223(96)00713-5"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.1.7-14.1994"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.47.100193.004231"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00087a014"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.3109\/07388559609147420"},{"key":"e_1_2_6_7_1","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1128\/aem.57.1.260-266.1991","article-title":"Ramos\u2010Gonzalez, M.\u2010I. Survival in soils of an herbicide\u2010resistant Pseudomonas putida strain bearing a recombinant TOL plasmid","volume":"57","author":"Ramos J. L.","year":"1991","journal-title":"Appl. Environ. Microbiol."},{"key":"e_1_2_6_8_1","doi-asserted-by":"crossref","first-page":"2835","DOI":"10.1128\/aem.61.8.2835-2839.1995","article-title":"Validation of microcosms for examining the survival of Pseudomonas aureofaciens (lac ZY) in soil","volume":"61","author":"Scott Angle J.","year":"1995","journal-title":"Appl. Environ. Microbiol."},{"key":"e_1_2_6_9_1","first-page":"81","volume-title":"Bioremediation: Principles and Practice Vol. III : Bioremediation Technologies","author":"Shanker R.","year":"1998"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/bp9600389"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.48.100194.002521"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/es00012a741"}],"container-title":["Biotechnology Progress"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1021%2Fbp990097x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/pdf\/10.1021\/bp990097x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:03:38Z","timestamp":1760054618000},"score":1,"resource":{"primary":{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/10.1021\/bp990097x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,1]]},"references-count":11,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1999,1]]}},"alternative-id":["10.1021\/bp990097x"],"URL":"https:\/\/doi.org\/10.1021\/bp990097x","archive":["Portico"],"relation":{},"ISSN":["8756-7938","1520-6033"],"issn-type":[{"value":"8756-7938","type":"print"},{"value":"1520-6033","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,1]]}}}