{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T22:24:08Z","timestamp":1777415048457,"version":"3.51.4"},"reference-count":52,"publisher":"American Chemical Society (ACS)","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ACS Chem. Biol."],"published-print":{"date-parts":[[2008,9,19]]},"DOI":"10.1021\/cb800085g","type":"journal-article","created":{"date-parts":[[2008,7,25]],"date-time":"2008-07-25T07:00:42Z","timestamp":1216969242000},"page":"542-554","source":"Crossref","is-referenced-by-count":40,"title":["Gatekeeping <i>versus<\/i> Promiscuity in the Early Stages of the Andrimid Biosynthetic Assembly Line"],"prefix":"10.1021","volume":"3","author":[{"given":"Nathan A.","family":"Magarvey","sequence":"first","affiliation":[{"name":"Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pascal D.","family":"Fortin","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul M.","family":"Thomas","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Illinois, Urbana\u2212Champaign, Illinois 61801"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neil L.","family":"Kelleher","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Illinois, Urbana\u2212Champaign, Illinois 61801"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher T.","family":"Walsh","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"316","published-online":{"date-parts":[[2008,7,25]]},"reference":[{"key":"ref1\/cit1","doi-asserted-by":"crossref","first-page":"1805","DOI":"10.1126\/science.1094318","volume":"303","author":"Walsh C. T.","year":"2004","journal-title":"Science (New York, NY)"},{"key":"ref2\/cit2","doi-asserted-by":"crossref","first-page":"3468","DOI":"10.1021\/cr0503097","volume":"106","author":"Fischbach M. A.","year":"2006","journal-title":"Chem. Rev."},{"key":"ref3\/cit3","doi-asserted-by":"crossref","first-page":"4409","DOI":"10.1021\/ja00248a055","volume":"109","author":"Fredenhagen A.","year":"1987","journal-title":"J. Am. Chem. Soc."},{"key":"ref4\/cit4","doi-asserted-by":"crossref","first-page":"2058","DOI":"10.1021\/jo00087a020","volume":"59","author":"Needham J.","year":"1994","journal-title":"J. Org. Chem."},{"key":"ref5\/cit5","doi-asserted-by":"crossref","first-page":"26066","DOI":"10.1074\/jbc.M402989200","volume":"279","author":"Freiberg C.","year":"2004","journal-title":"J. Biol. Chem."},{"key":"ref6\/cit6","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1016\/j.bmcl.2004.12.002","volume":"15","author":"Pohlmann J.","year":"2005","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"ref7\/cit7","doi-asserted-by":"crossref","first-page":"10660","DOI":"10.1021\/ja063194c","volume":"128","author":"Jin M.","year":"2006","journal-title":"J. Am. Chem. Soc."},{"key":"ref8\/cit8","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1038\/nature06068","volume":"448","author":"Fortin P. D.","year":"2007","journal-title":"Nature"},{"key":"ref9\/cit9","doi-asserted-by":"crossref","first-page":"6062","DOI":"10.1021\/ja034609m","volume":"125","author":"Christenson S. D.","year":"2003","journal-title":"J. Am. Chem. Soc."},{"key":"ref10\/cit10","doi-asserted-by":"crossref","first-page":"21810","DOI":"10.1074\/jbc.M703439200","volume":"282","author":"Rachid S.","year":"2007","journal-title":"J. Biol. Chem."},{"key":"ref11\/cit11","doi-asserted-by":"crossref","first-page":"53947","DOI":"10.1074\/jbc.M411215200","volume":"279","author":"Walker K. D.","year":"2004","journal-title":"J. Biol. Chem."},{"key":"ref12\/cit12","doi-asserted-by":"crossref","first-page":"15744","DOI":"10.1021\/ja0762689","volume":"129","author":"Christianson C. V.","year":"2007","journal-title":"J. Am. Chem. Soc."},{"key":"ref13\/cit13","doi-asserted-by":"crossref","first-page":"29633","DOI":"10.1074\/jbc.M605887200","volume":"281","author":"Van Lanen S. G.","year":"2006","journal-title":"J. Biol. Chem."},{"key":"ref14\/cit14","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1016\/S1074-5521(99)80082-9","volume":"6","author":"Stachelhaus T.","year":"1999","journal-title":"Chem. Biol."},{"key":"ref15\/cit15","doi-asserted-by":"crossref","first-page":"6000","DOI":"10.1021\/bi970006+","volume":"36","author":"Crump M. P.","year":"1997","journal-title":"Biochemistry"},{"key":"ref16\/cit16","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1126\/science.1122928","volume":"312","author":"Koglin A.","year":"2006","journal-title":"Science (New York, NY)"},{"key":"ref17\/cit17","doi-asserted-by":"crossref","first-page":"4648","DOI":"10.1021\/bi0274120","volume":"42","author":"Li Q.","year":"2003","journal-title":"Biochemistry"},{"key":"ref18\/cit18","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/S0969-2126(00)00120-9","volume":"8","author":"Weber T.","year":"2000","journal-title":"Structure"},{"key":"ref19\/cit19","doi-asserted-by":"crossref","first-page":"15874","DOI":"10.1074\/jbc.M112300200","volume":"277","author":"Wong H. C.","year":"2002","journal-title":"J. Biol. Chem."},{"key":"ref20\/cit20","doi-asserted-by":"crossref","first-page":"11002","DOI":"10.1021\/ja063238h","volume":"128","author":"Lai J. R.","year":"2006","journal-title":"J. Am. Chem. Soc."},{"key":"ref21\/cit21","doi-asserted-by":"crossref","first-page":"14869","DOI":"10.1021\/bi061979p","volume":"45","author":"Lai J. R.","year":"2006","journal-title":"Biochemistry"},{"key":"ref22\/cit22","doi-asserted-by":"crossref","first-page":"1585","DOI":"10.1021\/bi9719861","volume":"37","author":"Quadri L. E.","year":"1998","journal-title":"Biochemistry"},{"key":"ref23\/cit23","doi-asserted-by":"crossref","first-page":"12756","DOI":"10.1021\/bi061169d","volume":"45","author":"Dorrestein P. C.","year":"2006","journal-title":"Biochemistry"},{"key":"ref24\/cit24","doi-asserted-by":"crossref","first-page":"1356","DOI":"10.1021\/np0499261","volume":"67","author":"Chang Z.","year":"2004","journal-title":"J. Nat. Prod."},{"key":"ref25\/cit25","doi-asserted-by":"crossref","first-page":"1506","DOI":"10.1073\/pnas.0610503104","volume":"104","author":"Butcher R. A.","year":"2007","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"ref26\/cit26","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/0378-1119(94)90351-4","volume":"142","author":"Bibb M. J.","year":"1994","journal-title":"Gene"},{"key":"ref27\/cit27","doi-asserted-by":"crossref","first-page":"1206","DOI":"10.1002\/cbic.200600075","volume":"7","author":"Simunovic V.","year":"2006","journal-title":"ChemBioChem"},{"key":"ref28\/cit28","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1126\/science.287.5453.640","volume":"287","author":"Tang L.","year":"2000","journal-title":"Science (New York, NY)"},{"key":"ref29\/cit29","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1016\/S1074-5521(00)00044-2","volume":"7","author":"Piel J.","year":"2000","journal-title":"Chem. Biol."},{"key":"ref30\/cit30","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/S0378-1119(02)00860-0","volume":"296","author":"Chang Z.","year":"2002","journal-title":"Gene"},{"key":"ref31\/cit31","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1128\/jb.171.2.791-798.1989","volume":"171","author":"Liu J.","year":"1989","journal-title":"J. Bacteriol."},{"key":"ref32\/cit32","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/0378-1119(93)90221-N","volume":"133","author":"Bender C. L.","year":"1993","journal-title":"Gene"},{"key":"ref33\/cit33","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1126\/science.277.5324.367","volume":"277","author":"Jacobsen J. R.","year":"1997","journal-title":"Science (New York, NY)"},{"key":"ref34\/cit34","doi-asserted-by":"crossref","first-page":"1171","DOI":"10.1016\/S0968-0896(98)00081-9","volume":"6","author":"Jacobsen J. R.","year":"1998","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"ref35\/cit35","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1039\/B707678A","volume":"25","author":"Kennedy J.","year":"2008","journal-title":"Nat. Prod. Rep."},{"key":"ref36\/cit36","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1007\/s00253-005-1891-8","volume":"68","author":"Weist S.","year":"2005","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref37\/cit37","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1039\/b003939j","volume":"19","author":"Moore B. S.","year":"2002","journal-title":"Nat. Prod. Rep."},{"key":"ref38\/cit38","doi-asserted-by":"crossref","first-page":"9290","DOI":"10.1021\/ja035973o","volume":"125","author":"Kalaitzis J. A.","year":"2003","journal-title":"J. Am. Chem. Soc."},{"key":"ref39\/cit39","doi-asserted-by":"crossref","first-page":"980","DOI":"10.1038\/79479","volume":"18","author":"Cropp T. A.","year":"2000","journal-title":"Nat. Biotechnol."},{"key":"ref40\/cit40","doi-asserted-by":"crossref","first-page":"1202","DOI":"10.1002\/anie.200461990","volume":"44","author":"Ziehl M.","year":"2005","journal-title":"Angew. Chem., Int. Ed."},{"key":"ref41\/cit41","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1038\/nrm1014","volume":"4","author":"Lorand L.","year":"2003","journal-title":"Nat. Rev. Mol. Cell. Biol."},{"key":"ref42\/cit42","doi-asserted-by":"crossref","first-page":"1756","DOI":"10.1021\/bi00576a019","volume":"18","author":"Lorand L.","year":"1979","journal-title":"Biochemistry"},{"key":"ref43\/cit43","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1093\/protein\/gzh015","volume":"17","author":"Taki M.","year":"2004","journal-title":"Protein Eng., Des. Sel."},{"key":"ref44\/cit44","doi-asserted-by":"crossref","first-page":"6230","DOI":"10.1021\/jf000302k","volume":"48","author":"Ohtsuka T.","year":"2000","journal-title":"J. Agric. Food Chem."},{"key":"ref45\/cit45","doi-asserted-by":"crossref","first-page":"11951","DOI":"10.1073\/pnas.0705348104","volume":"104","author":"Fischbach M. A.","year":"2007","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"ref46\/cit46","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1126\/science.284.5413.486","volume":"284","author":"Belshaw P. J.","year":"1999","journal-title":"Science (New York, NY)"},{"key":"ref47\/cit47","doi-asserted-by":"crossref","first-page":"42020","DOI":"10.1074\/jbc.M305339200","volume":"278","author":"Watanabe K.","year":"2003","journal-title":"J. Biol. Chem."},{"key":"ref48\/cit48","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook J.","year":"2000","edition":"3"},{"key":"ref49\/cit49","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","volume":"72","author":"Bradford M. M.","year":"1976","journal-title":"Anal. Biochem."},{"key":"ref50\/cit50","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1002\/bspy.350010604","volume":"1","author":"Tonge P. J.","year":"1995","journal-title":"Biospectroscopy"},{"key":"ref51\/cit51","doi-asserted-by":"crossref","first-page":"6640","DOI":"10.1073\/pnas.120163297","volume":"97","author":"Datsenko K. A.","year":"2000","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"ref52\/cit52","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1093\/genetics\/122.3.491","volume":"122","author":"Sampson B. A.","year":"1989","journal-title":"Genetics"}],"container-title":["ACS Chemical Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/cb800085g","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,3,7]],"date-time":"2023-03-07T22:52:19Z","timestamp":1678229539000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/doi\/10.1021\/cb800085g"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,7,25]]},"references-count":52,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2008,9,19]]}},"alternative-id":["10.1021\/cb800085g"],"URL":"https:\/\/doi.org\/10.1021\/cb800085g","relation":{},"ISSN":["1554-8929","1554-8937"],"issn-type":[{"value":"1554-8929","type":"print"},{"value":"1554-8937","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,7,25]]}}}