{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T05:59:35Z","timestamp":1785477575343,"version":"3.56.0"},"reference-count":19,"publisher":"American Chemical Society (ACS)","issue":"7","license":[{"start":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T00:00:00Z","timestamp":1772582400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000266","name":"Engineering and Physical Sciences Research Council","doi-asserted-by":"publisher","award":["EP\/X031179\/1"],"award-info":[{"award-number":["EP\/X031179\/1"]}],"id":[{"id":"10.13039\/501100000266","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000741","name":"University of Warwick","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/501100000741","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002283","name":"Alzheimer?s Research UK","doi-asserted-by":"publisher","award":["ARUK-RADF2022B-008"],"award-info":[{"award-number":["ARUK-RADF2022B-008"]}],"id":[{"id":"10.13039\/501100002283","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,27]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Correlative microscopy linking synchrotron X-ray fluorescence (SXRF) with optical imaging is valuable for contextualizing chemical element distributions in biology. The spatial correlation necessary to achieve this presents fundamental challenges and can be a significant constraint on accuracy and data interpretation. We present a technical solution based on a finder grid concept, optimized for SXRF correlative studies of metals in biological tissues, with scope for wider adaptation and application. A hierarchically patterned fiducial system was directly etched onto spectroscopically clean quartz substrates via femtosecond laser ablation. This design enables improved correlation among SXRF, optical imaging, and histological staining over a greater range of length scales than conventional registration methods such as the use of tissue architecture from serial sections and the use of electron-microscopy-resolution finder grids and applied fiduciary markers that can introduce XRF-signal-dominating levels of elements such as copper, nickel, gold, and titanium. We present two quartz finder grid formats: a microgrid and a nanogrid design. We demonstrate their utility for rapid ROI relocalization and same-section correlative workflows using human brain tissue. The etched quartz finder grid approach facilitates rapid and reproducible ROI relocalization and alignment across instruments, particularly where integral fiducial markers are sparse or ambiguous.<\/jats:p>","DOI":"10.1021\/cbmi.5c00231","type":"journal-article","created":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T21:25:06Z","timestamp":1772659506000},"page":"1538-1546","source":"Crossref","is-referenced-by-count":1,"title":["A Hierarchical Etched\nGrid for Correlative Chemical\nImaging"],"prefix":"10.1021","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-4223-4648","authenticated-orcid":true,"given":"Natalie S. W.","family":"Arigundiya","sequence":"first","affiliation":[{"name":"University of Warwick , , ,","place":["Coventry, United Kingdom, CV4 7AL"]},{"name":"University of Warwick , , ,","place":["Coventry, United Kingdom, CV4 7AL"]},{"name":"Diamond Light Source , Harwell Science and Innovation Campus , ,","place":["Didcot, United Kingdom, OX11 0DE"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4035-0369","authenticated-orcid":true,"given":"Jake","family":"Brooks","sequence":"additional","affiliation":[{"name":"University of Warwick , , ,","place":["Coventry, United Kingdom, CV4 7AL"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Sykes","sequence":"additional","affiliation":[{"name":"Scitech Precision Limited , , Harwell Science and Innovation Campus , ,","place":["Didcot, United Kingdom, OX11 0QX"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seojin","family":"Lee","sequence":"additional","affiliation":[{"name":"University of Toronto , , , ,","place":["Toronto, Ontario, Canada, M5T 0S8"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naomi P.","family":"Visanji","sequence":"additional","affiliation":[{"name":"University of Toronto , , , ,","place":["Toronto, Ontario, Canada, M5T 0S8"]},{"name":"University of Toronto , , , ,","place":["Toronto, ON, Canada, M5T 0S8"]},{"name":"University Health Network , , , ,","place":["Toronto, ON, Canada, M5T 0S8"]},{"name":"Toronto Western Hospital , , , ,","place":["Toronto, ON, Canada, M5T 2S8"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher M.","family":"Morris","sequence":"additional","affiliation":[{"name":"Newcastle University , , ,","place":["Newcastle-upon-Tyne, United Kingdom, NE4 5PL"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kalotina","family":"Geraki","sequence":"additional","affiliation":[{"name":"Diamond Light Source , Harwell Science and Innovation Campus , ,","place":["Didcot, United Kingdom, OX11 0DE"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8423-4183","authenticated-orcid":true,"given":"Joanna F.","family":"Collingwood","sequence":"additional","affiliation":[{"name":"University of Warwick , , ,","place":["Coventry, United Kingdom, CV4 7AL"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,3,4]]},"reference":[{"key":"2026073101213383000_cit1","doi-asserted-by":"publisher","first-page":"191","DOI":"10.3389\/fphar.2014.00191","article-title":"The role of iron in neurodegenerative disorders: Insights\nand opportunities with synchrotron light","volume":"5","author":"Collingwood","year":"2014","journal-title":"Front.\nPharmacol."},{"key":"2026073101213383000_cit2","unstructured":"Finnegan, M. E.\n          \n          Investigation\nof the Relationship Between Iron and High Field MRI in Healthy and\nAlzheimer\u2019s Disease Tissue. Ph.D. Thesis, University\nof Warwick, Warwick, U.K., 2013. https:\/\/wrap.warwick.ac.uk\/60293\/ (accessed Jan 9, 2026)."},{"issue":"6","key":"2026073101213383000_cit3","doi-asserted-by":"publisher","first-page":"1935","DOI":"10.1107\/S1600577521008213","article-title":"NanoMAX: The hard X-ray nanoprobe beamline at the MAX IV Laboratory","volume":"28","author":"Johansson","year":"2021","journal-title":"J. Synchrotron Radiat."},{"key":"2026073101213383000_cit4","unstructured":"Lightsources.org. 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U.S.A."},{"issue":"1","key":"2026073101213383000_cit6","doi-asserted-by":"publisher","first-page":"1024","DOI":"10.1038\/s42003-024-06636-1","article-title":"Nanoscale\nsynchrotron X-ray analysis of intranuclear iron in melanised neurons\nof Parkinson\u2019s substantia nigra","volume":"7","author":"Brooks","year":"2024","journal-title":"Commun.\nBiol."},{"issue":"8","key":"2026073101213383000_cit7","doi-asserted-by":"publisher","first-page":"721","DOI":"10.1039\/c2mt20052j","article-title":"Metal imaging in\nneurodegenerative diseases","volume":"4","author":"Bourassa","year":"2012","journal-title":"Metallomics"},{"key":"2026073101213383000_cit8","doi-asserted-by":"publisher","DOI":"10.1016\/j.sab.2024.107060","article-title":"Diffraction\npeak identification and correction in EDXRF spectroscopy","volume":"221","author":"Said Sadeg","year":"2024","journal-title":"Spectrochim. 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Protoc."},{"key":"2026073101213383000_cit11","doi-asserted-by":"publisher","first-page":"1014","DOI":"10.3389\/fnins.2019.01014","article-title":"Mapping chemical\nelements and iron\noxidation states in the substantia nigra of 6-hydroxydopamine lesioned\nrats using correlative immunohistochemistry with proton and synchrotron\nmicro-analysis","volume":"13","author":"Carmona","year":"2019","journal-title":"Front. Neurosci."},{"key":"2026073101213383000_cit12","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2023.102372","article-title":"Multimodal\nand multiscale correlative\nelemental imaging: From whole tissues down to organelles","volume":"76","author":"Roudeau","year":"2023","journal-title":"Curr. Opin. Chem. 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Chem."},{"key":"2026073101213383000_cit16","unstructured":"UQG Optics Ltd.\n          . Fused Quartz Glass. https:\/\/www.uqgoptics.com\/materials\/fused-quartz\/ (accessed Nov 13, 2025)."},{"key":"2026073101213383000_cit17","unstructured":"Humeau.\n          . LABSOLUTE\u00ae Microscope Slides Made from Soda-Lime\nGlass (technical data sheet). https:\/\/www.humeau.com\/media\/blfa_files\/TC_Lames-microscope_82707695001_FR_120320.pdf (accessed Nov 13, 2025)."},{"key":"2026073101213383000_cit18","unstructured":"Australian Nuclear Science and Technology Organisation (ANSTO).\n          . Sample Guide\u2500X-ray Fluorescence Microscopy (XFM) Beamline. Australian Synchrotron. https:\/\/www.ansto.gov.au\/our-facilities\/australian-synchrotron\/synchrotron-beamlines\/x-ray-fluorescence-microscopy\/sample (accessed Jan 9, 2026)."},{"key":"2026073101213383000_cit19","unstructured":"European Synchrotron Radiation Facility (ESRF).\n          . 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