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BTD-4AS shows pronounced solvatochromism, viscosity- and temperature-dependent emission, and large Stokes shifts, consistent with a twisted intramolecular charge transfer state supported by Lippert-Mataga and Reichardt analyses. Its fluorescence is strongly pH-sensitive (maximum near pH \u22486\u20138) and markedly enhanced in micellar and viscous media. The dye\u2019s photostability and lipophilicity support its use as a versatile probe for polarity, viscosity, pH, and lipid-rich environments. Building on these properties, BTD-4AS enables selective cellular bioimaging of lipid droplets and, in a mouse model, reports liver injury through polarity\/viscosity-dependent spectral shifts\u2500red emission in damaged regions exposed to extracellular milieu and green emission in intact lipid-rich cells.<\/jats:p>","DOI":"10.1021\/cbmi.5c00242","type":"journal-article","created":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T21:22:23Z","timestamp":1769548943000},"page":"1432-1454","source":"Crossref","is-referenced-by-count":1,"title":["Lipid-Targeting Multiresponsive\nBenzothiadiazole Fluorophore\nfor Live-Cell Imaging and Real-Time Liver Injury Monitoring"],"prefix":"10.1021","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3783-9283","authenticated-orcid":true,"given":"Brenno A. 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