{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T13:00:30Z","timestamp":1790600430616,"version":"4.1.0"},"reference-count":26,"publisher":"American Chemical Society (ACS)","issue":"9","license":[{"start":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T00:00:00Z","timestamp":1775433600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["GK202502002"],"award-info":[{"award-number":["GK202502002"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["GK202506027"],"award-info":[{"award-number":["GK202506027"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22274093"],"award-info":[{"award-number":["22274093"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22304109"],"award-info":[{"award-number":["22304109"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22374094"],"award-info":[{"award-number":["22374094"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22574098"],"award-info":[{"award-number":["22574098"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Youth Innovation Team of Shaanxi Universities"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The development of a sensitive and accurate method for the determination of cardiac troponin I (cTnI), as an ideal biomarker of acute myocardial infarction (AMI), is critical for the early diagnosis of AMI. In this work, a tetrahedral DNA framework (TDF)-bridged aptasensing method was proposed for the ultrasensitive determination of cTnI via electrogenerated chemiluminescence (ECL) and fluorescence imaging methods, in which a specific anti-cTnI aptamer-tagged tetrahedral DNA framework (TDF-aptamer) was designed as a capture probe by incorporating a specific aptamer against cTnI within the three-dimensional TDF. The TDF-aptamer was modified onto a gold electrode surface through the Au\u2013S bond, providing a specifically captured platform for cTnI with a 3D binding space at the nanoscale. One microsized signal probe (Ab2-biotin\u2022SA@MB-Ru1) was synthesized by using magnetic Fe3O4 as carriers to load a large amount of ruthenium complex and a specific cTnI antibody. After the sandwich assay among the modified TDF-aptamer, cTnI, and the signal probe, both ECL and fluorescence signals were recorded for the cTnI assay with desirable selectivity and high sensitivity (linear range of 5 to 80 pg\/mL using ECL intensity detection and 0.5 to 500 pg\/mL using ECL and fluorescence imaging methods). This work demonstrated that the combination of the specific TDF-aptamer and microsized Ab2-biotin\u2022SA@MB-Ru1 within the imaging method can enhance the specific recognition and detection sensitivity for cTnI, which is promising for AMI diagnosis.<\/jats:p>","DOI":"10.1021\/cbmi.5c00292","type":"journal-article","created":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T08:04:08Z","timestamp":1775462648000},"page":"2065-2073","source":"Crossref","is-referenced-by-count":0,"title":["Tetrahedral DNA Framework\u2013Bridged\nAptasensing\nMethod for Ultrasensitive Determination of Cardiac Troponin I"],"prefix":"10.1021","volume":"4","author":[{"given":"Jiaqi","family":"Cai","sequence":"first","affiliation":[{"name":"Shaanxi Normal University , , , ,","place":["Xi\u2019an, P. R. China, 710062"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenshuai","family":"Zhou","sequence":"additional","affiliation":[{"name":"Shaanxi Normal University , , , ,","place":["Xi\u2019an, P. R. China, 710062"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Gao","sequence":"additional","affiliation":[{"name":"Xi\u2019an People\u2019s Hospital (Xi\u2019an Fourth Hospital) , , ,","place":["Xi\u2019an, P. R. China, 710068"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3420-3215","authenticated-orcid":true,"given":"Xiaolin","family":"Yang","sequence":"additional","affiliation":[{"name":"Shaanxi Normal University , , , ,","place":["Xi\u2019an, P. R. China, 710062"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Li","sequence":"additional","affiliation":[{"name":"Shaanxi Normal University , , , ,","place":["Xi\u2019an, P. R. China, 710062"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2829-5122","authenticated-orcid":true,"given":"Chengxiao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shaanxi Normal University , , , ,","place":["Xi\u2019an, P. R. China, 710062"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2268-0073","authenticated-orcid":true,"given":"Honglan","family":"Qi","sequence":"additional","affiliation":[{"name":"Shaanxi Normal University , , , ,","place":["Xi\u2019an, P. R. China, 710062"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,4,6]]},"reference":[{"key":"2026092808070883600_cit1","doi-asserted-by":"publisher","first-page":"858","DOI":"10.3390\/biom15060858","article-title":"Recent advances in monitoring technologies\nfor cardiac troponin I: A pivotal biomarker in cardiovascular diseases","volume":"15","author":"Zhang","year":"2025","journal-title":"Biomolecules"},{"key":"2026092808070883600_cit2","doi-asserted-by":"publisher","first-page":"399","DOI":"10.1021\/acssensors.0c01681","article-title":"Gradient-based\nrapid digital immunoassay for high-sensitivity cardiac troponin T\n(hs-cTnT) detection in 1 \u03bcL plasma","volume":"6","author":"Jing","year":"2021","journal-title":"ACS\nSens."},{"key":"2026092808070883600_cit3","doi-asserted-by":"publisher","first-page":"1358","DOI":"10.1016\/j.amjmed.2017.07.033","article-title":"Using high-sensitivity cardiac troponin T for acute\ncardiac care","volume":"130","author":"Sandoval","year":"2017","journal-title":"Am. 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Eng., C"}],"container-title":["Chemical &amp; Biomedical\nImaging"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/cbmi.5c00292","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/cbihbp\/article-pdf\/4\/9\/2065\/66261155\/cbmi.5c00292.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/cbihbp\/article-pdf\/4\/9\/2065\/66261155\/cbmi.5c00292.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T12:30:56Z","timestamp":1790598656000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/cbihbp\/article\/4\/9\/2065\/5232780\/Tetrahedral-DNA-Framework-Bridged-Aptasensing"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,6]]},"references-count":26,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,4,6]]},"published-print":{"date-parts":[[2026,9,28]]}},"URL":"https:\/\/doi.org\/10.1021\/cbmi.5c00292","relation":{},"ISSN":["2832-3637"],"issn-type":[{"value":"2832-3637","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,6]]}}}