{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T13:00:19Z","timestamp":1790600419780,"version":"4.1.0"},"reference-count":17,"publisher":"American Chemical Society (ACS)","issue":"9","license":[{"start":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T00:00:00Z","timestamp":1784678400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"American Lebanese Syrian Associated Charities (ALSAC) of St Jude Children?s Research Hospital"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The quantitative evaluation of therapeutics in the drug discovery pipeline is essential for elucidating their pharmacokinetic and pharmacodynamic profiles within complex biological systems. Traditionally, autoradiography and liquid chromatography-mass spectrometry (LC-MS) have been employed for such analyses. However, matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) is emerging as a promising alternative, offering a more rapid untargeted workflow. Quantitative MALDI-MSI (qMSI) relies on accurate calibration to achieve robust and reproducible quantification of analytes. Here, we present an open-source software tool, SpotOn, designed to facilitate the discrete deposition of low-volume calibration standards onto glass slides or tissue sections. By enabling precise placement and significantly reducing the volume of standards required, our tool affords the generation of more calibration points across a wider dynamic range, thereby improving the robustness and accuracy of quantitative analyses. Software was validated using JQ1 on both glass slides and murine liver tissue. The resulting calibration curves demonstrated strong linearity, with coefficients of determination (R2) exceeding 0.92. This tool streamlines the qMSI workflow, conserves valuable reagents, and enhances analytical reliability, making it a helpful resource.<\/jats:p>","DOI":"10.1021\/cbmi.6c00010","type":"journal-article","created":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T23:45:13Z","timestamp":1784763913000},"page":"2119-2125","source":"Crossref","is-referenced-by-count":0,"title":["SpotOn: A Tool to Enhance\nEcho Acoustic Standard Deposition\nfor Quantitative Mass Spectrometry Imaging"],"prefix":"10.1021","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1668-3227","authenticated-orcid":true,"given":"Emily R.","family":"Sekera","sequence":"first","affiliation":[{"name":"St. Jude Children\u2019s Research Hospital , , , ,","place":["Memphis, Tennessee, United States, 38105"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1738-6387","authenticated-orcid":true,"given":"Nathaniel","family":"Twarog","sequence":"additional","affiliation":[{"name":"St. Jude Children\u2019s Research Hospital , , , ,","place":["Memphis, Tennessee, United States, 38105"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebekah","family":"DeVries","sequence":"additional","affiliation":[{"name":"St. Jude Children\u2019s Research Hospital , , , ,","place":["Memphis, Tennessee, United States, 38105"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. Jake","family":"Slavish","sequence":"additional","affiliation":[{"name":"St. Jude Children\u2019s Research Hospital , , , ,","place":["Memphis, Tennessee, United States, 38105"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John J.","family":"Bowling","sequence":"additional","affiliation":[{"name":"St. Jude Children\u2019s Research Hospital , , , ,","place":["Memphis, Tennessee, United States, 38105"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6266-2910","authenticated-orcid":true,"given":"Anang A.","family":"Shelat","sequence":"additional","affiliation":[{"name":"St. Jude Children\u2019s Research Hospital , , , ,","place":["Memphis, Tennessee, United States, 38105"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,7,22]]},"reference":[{"issue":"12","key":"2026092808074129700_cit1","doi-asserted-by":"publisher","first-page":"899","DOI":"10.1038\/s41573-022-00472-w","article-title":"Phenotypic drug discovery: recent successes, lessons\nlearned and new directions","volume":"21","author":"Vincent","year":"2022","journal-title":"Nat. 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Sci."},{"issue":"9","key":"2026092808074129700_cit5","doi-asserted-by":"publisher","first-page":"963","DOI":"10.1177\/2472555220941843","article-title":"A Critical\nand Concise Review of\nMass Spectrometry Applied to Imaging in Drug Discovery","volume":"25","author":"Goodwin","year":"2020","journal-title":"SLAS Discov"},{"issue":"8","key":"2026092808074129700_cit6","doi-asserted-by":"publisher","DOI":"10.1002\/jms.4563","article-title":"The emergence\nof imaging mass spectrometry\nin drug discovery and development: Making a difference by driving\ndecision making","volume":"56","author":"Castellino","year":"2021","journal-title":"J. Mass Spectrom."},{"issue":"11","key":"2026092808074129700_cit7","doi-asserted-by":"publisher","first-page":"1099","DOI":"10.4155\/bio-2019-0035","article-title":"Quantification and assessment of\ndetection capability in imaging mass spectrometry using a revised\nmimetic tissue model","volume":"11","author":"Barry","year":"2019","journal-title":"Bioanalysis"},{"issue":"4","key":"2026092808074129700_cit8","doi-asserted-by":"publisher","DOI":"10.1002\/jms.4932","article-title":"Quantitative mass spectrometry imaging (qMSI): A tutorial","volume":"59","author":"Kibbe","year":"2024","journal-title":"J. Mass Spectrom."},{"issue":"9","key":"2026092808074129700_cit9","doi-asserted-by":"publisher","first-page":"3620","DOI":"10.1021\/acs.jproteome.0c00443","article-title":"Considerations for MALDI-Based Quantitative Mass Spectrometry\nImaging Studies","volume":"19","author":"Tobias","year":"2020","journal-title":"J. Proteome Res."},{"key":"2026092808074129700_cit10","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijms.2024.117219","article-title":"MALDI sample preparation\nmethods: A mini review","volume":"498","author":"Patil","year":"2024","journal-title":"Int. J. Mass Spectrom."},{"issue":"1","key":"2026092808074129700_cit11","doi-asserted-by":"publisher","first-page":"529","DOI":"10.1186\/s12859-017-1934-z","article-title":"ImageJ2: ImageJ for the next generation\nof scientific image data","volume":"18","author":"Rueden","year":"2017","journal-title":"BMC Bioinform."},{"issue":"7","key":"2026092808074129700_cit12","doi-asserted-by":"publisher","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH Image\nto ImageJ: 25 years of\nimage analysis","volume":"9","author":"Schneider","year":"2012","journal-title":"Nat. Methods"},{"issue":"16","key":"2026092808074129700_cit13","doi-asserted-by":"publisher","first-page":"4893","DOI":"10.1016\/j.jprot.2012.04.012","article-title":"Sample\npreparation for mass spectrometry imaging: Small mistakes can lead\nto big consequences","volume":"75","author":"Goodwin","year":"2012","journal-title":"Journal of Proteomics"},{"issue":"12","key":"2026092808074129700_cit14","doi-asserted-by":"publisher","first-page":"2728","DOI":"10.1021\/jasms.1c00081","article-title":"Factorial Design to Optimize Matrix\nSpraying Parameters for MALDI Mass Spectrometry Imaging","volume":"32","author":"Tressler","year":"2021","journal-title":"J. Am. Soc. Mass Spectrom."},{"key":"2026092808074129700_cit15","doi-asserted-by":"publisher","DOI":"10.26434\/chemrxiv-2025-9n29g","article-title":"Development\nof Workflows for Discrete Standard Deposition Using Echo Acoustic\nLiquid Handlers for qMSI","author":"Sekera","year":"2025","journal-title":"ChemRxiv"},{"issue":"3","key":"2026092808074129700_cit16","doi-asserted-by":"publisher","first-page":"654","DOI":"10.1039\/C7AN01828B","article-title":"Quantitative mass\nspectrometry imaging of glutathione in healthy and cancerous hen ovarian\ntissue sections by infrared matrix-assisted laser desorption electrospray\nionization (IR-MALDESI)","volume":"143","author":"Nazari","year":"2018","journal-title":"Analyst"},{"key":"2026092808074129700_cit17","doi-asserted-by":"publisher","first-page":"S0006","DOI":"10.5702\/massspectrometry.S0006","article-title":"MALDI and\nRelated Methods: A Solved Problem or Still a Mystery?","volume":"2","author":"Knochenmuss","year":"2013","journal-title":"Mass Spectrom (Tokyo)"}],"container-title":["Chemical &amp; Biomedical\nImaging"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/cbmi.6c00010","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/cbihbp\/article-pdf\/4\/9\/2119\/66162440\/cbmi.6c00010.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/cbihbp\/article-pdf\/4\/9\/2119\/66162440\/cbmi.6c00010.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T12:30:16Z","timestamp":1790598616000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/cbihbp\/article\/4\/9\/2119\/5228141\/SpotOn-A-Tool-to-Enhance-Echo-Acoustic-Standard"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,22]]},"references-count":17,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,7,22]]},"published-print":{"date-parts":[[2026,9,28]]}},"URL":"https:\/\/doi.org\/10.1021\/cbmi.6c00010","relation":{},"ISSN":["2832-3637"],"issn-type":[{"value":"2832-3637","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,22]]}}}