{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T13:00:28Z","timestamp":1790600428344,"version":"4.1.0"},"reference-count":31,"publisher":"American Chemical Society (ACS)","issue":"9","license":[{"start":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T00:00:00Z","timestamp":1775088000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Beijing Changping Laboratory","id":[{"id":"https:\/\/ror.org\/04kf84k17","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Label-free coherent Raman scattering (CRS) microscopy enables chemical observation of intricate cellular and intercellular structures of biological tissues with submicron resolution and has become the most prominent stain-free alternative to gold-standard hematoxylin and eosin (H&amp;E) histology. However, the imaging field of view (FOV) of current CRS microscopes is typically limited to less than a millimeter. Various attempts in instrumentation have been made to perform large-scale CRS mapping across an intact tissue, but the costly trade-offs are either imaging speed or spatial resolution. To address this challenge, we developed a four-channel coherent anti-Stokes Raman scattering (CARS) microscope, offering submicron imaging of 13 \u00d7 13 mm2 large tissues within 100 s. Four miniaturized CARS systems, each equipped with a microelectromechanical system (MEMS) scanner, a micro-objective, and a collection fiber bundle, were fully integrated to perform ultrafast chemical mapping of intact unprocessed tissues with an electronically synchronized motorized stage. We demonstrated its capability by performing large-scale CARS imaging of mouse brain tissues and human glioma tissues. This label-free technique may pave the way for future clinical applications of stain-free pathology in the operating room.<\/jats:p>","DOI":"10.1021\/cbmi.6c00016","type":"journal-article","created":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T16:04:32Z","timestamp":1775145872000},"page":"2084-2090","source":"Crossref","is-referenced-by-count":0,"title":["100-Second Large-Area\nChemical Imaging by Integrated\nFour-Channel CARS Microscopy"],"prefix":"10.1021","volume":"4","author":[{"given":"Wei","family":"Ju","sequence":"first","affiliation":[{"name":"Huazhong University of Science and Technology , , , ,","place":["Wuhan, Hubei, China, 430074"]},{"name":"Changping Laboratory , ,","place":["Beijing, China, 102206"]},{"name":"Huazhong University of Science and Technology , , ,","place":["Wuhan, China, 430074"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiliang","family":"Huang","sequence":"additional","affiliation":[{"name":"Huazhong University of Science and Technology , , , ,","place":["Wuhan, Hubei, China, 430074"]},{"name":"Changping Laboratory , ,","place":["Beijing, China, 102206"]},{"name":"Huazhong University of Science and Technology , , ,","place":["Wuhan, China, 430074"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shangyu","family":"Li","sequence":"additional","affiliation":[{"name":"Changping Laboratory , ,","place":["Beijing, China, 102206"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunshan","family":"Zhong","sequence":"additional","affiliation":[{"name":"Changping Laboratory , ,","place":["Beijing, China, 102206"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiran","family":"Li","sequence":"additional","affiliation":[{"name":"Huazhong University of Science and Technology , , , ,","place":["Wuhan, Hubei, China, 430074"]},{"name":"Changping Laboratory , ,","place":["Beijing, China, 102206"]},{"name":"Huazhong University of Science and Technology , , ,","place":["Wuhan, China, 430074"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Li","sequence":"additional","affiliation":[{"name":"Huazhong University of Science and Technology , , , ,","place":["Wuhan, Hubei, China, 430074"]},{"name":"Changping Laboratory , ,","place":["Beijing, China, 102206"]},{"name":"Huazhong University of Science and Technology , , ,","place":["Wuhan, China, 430074"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunxian","family":"Yang","sequence":"additional","affiliation":[{"name":"Changping Laboratory , ,","place":["Beijing, China, 102206"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Huang","sequence":"additional","affiliation":[{"name":"Changping Laboratory , ,","place":["Beijing, China, 102206"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai","family":"Wang","sequence":"additional","affiliation":[{"name":"Changping Laboratory , ,","place":["Beijing, China, 102206"]},{"name":"Huazhong University of Science and Technology , , ,","place":["Wuhan, China, 430074"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai","family":"Yan","sequence":"additional","affiliation":[{"name":"Changping Laboratory , ,","place":["Beijing, China, 102206"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1414-0947","authenticated-orcid":true,"given":"Jiangling","family":"Wan","sequence":"additional","affiliation":[{"name":"Huazhong University of Science and Technology , , , ,","place":["Wuhan, Hubei, China, 430074"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangliang","family":"Yang","sequence":"additional","affiliation":[{"name":"Huazhong University of Science and Technology , , , ,","place":["Wuhan, Hubei, China, 430074"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5168-6829","authenticated-orcid":true,"given":"Ping","family":"Wang","sequence":"additional","affiliation":[{"name":"Huazhong University of Science and Technology , , , ,","place":["Wuhan, Hubei, China, 430074"]},{"name":"Changping Laboratory , ,","place":["Beijing, China, 102206"]},{"name":"Huazhong University of Science and Technology , , ,","place":["Wuhan, China, 430074"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,4,2]]},"reference":[{"key":"2026092808071714700_cit1","doi-asserted-by":"publisher","first-page":"0027","DOI":"10.1038\/s41551-016-0027","article-title":"Rapid intraoperative histology of unprocessed surgical specimens\nvia fibre-laser-based stimulated Raman scattering microscopy","volume":"1","author":"Orringer","year":"2017","journal-title":"Nat. Biomed. Eng."},{"key":"2026092808071714700_cit2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adn3426","article-title":"Virtual formalin- fixed\nand paraffin- embedded staining of fresh\nbrain tissue via stimulated Raman CycleGAN model","volume":"10","author":"Liu","year":"2024","journal-title":"Sci. Adv."},{"key":"2026092808071714700_cit3","doi-asserted-by":"publisher","first-page":"7075","DOI":"10.1073\/pnas.0832308100","article-title":"Live tissue intrinsic\nemission microscopy using multiphoton-excited\nnative fluorescence and second harmonic generation","volume":"100","author":"Zipfel","year":"2003","journal-title":"Proc. Natl. Acad. Sci. U. S. 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Biotechnol."},{"key":"2026092808071714700_cit7","doi-asserted-by":"publisher","first-page":"138","DOI":"10.1038\/s41377-022-00836-2","article-title":"Real-time whole-brain\nimaging of hemodynamics and oxygenation at\nmicro-vessel resolution with ultrafast wide-field photoacoustic microscopy","volume":"11","author":"Zhu","year":"2022","journal-title":"Light Sci. Appl."},{"key":"2026092808071714700_cit8","doi-asserted-by":"publisher","first-page":"13081","DOI":"10.1073\/pnas.0406877102","article-title":"Nonlinear\nstructured-illumination microscopy: Wide-field fluorescence imaging\nwith theoretically unlimited resolution","volume":"102","author":"Gustafsson","year":"2005","journal-title":"Proc.\nNatl. Acad. Sci. U. S. 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Rev."},{"key":"2026092808071714700_cit15","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0107115","article-title":"Label-free delineation of brain tumors by coherent anti-Stokes Raman\nscattering microscopy in an orthotopic mouse model and human glioblastoma","volume":"9","author":"Uckermann","year":"2014","journal-title":"PLoS One"},{"key":"2026092808071714700_cit16","doi-asserted-by":"publisher","first-page":"404","DOI":"10.1002\/jbio.201500323","article-title":"Assessing the efficacy of coherent anti-Stokes Raman\nscattering microscopy\nfor the detection of infiltrating glioblastoma in fresh brain samples","volume":"10","author":"Galli","year":"2017","journal-title":"Journal of Biophotonics"},{"key":"2026092808071714700_cit17","doi-asserted-by":"publisher","first-page":"10073","DOI":"10.1038\/s41467-024-54429-1","article-title":"Super-resolved coherent\nanti-Stokes Raman scattering microscopy by coherent image scanning","volume":"15","author":"Zhitnitsky","year":"2024","journal-title":"Nat. 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Commun."}],"container-title":["Chemical &amp; Biomedical\nImaging"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/cbmi.6c00016","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/cbihbp\/article-pdf\/4\/9\/2084\/66261655\/cbmi.6c00016.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/cbihbp\/article-pdf\/4\/9\/2084\/66261655\/cbmi.6c00016.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T12:32:19Z","timestamp":1790598739000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/cbihbp\/article\/4\/9\/2084\/5232819\/100-Second-Large-Area-Chemical-Imaging-by"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,2]]},"references-count":31,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,4,2]]},"published-print":{"date-parts":[[2026,9,28]]}},"URL":"https:\/\/doi.org\/10.1021\/cbmi.6c00016","relation":{},"ISSN":["2832-3637"],"issn-type":[{"value":"2832-3637","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,2]]}}}