{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T16:58:44Z","timestamp":1758992324742},"reference-count":38,"publisher":"American Chemical Society (ACS)","issue":"24","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Inorg. Chem."],"published-print":{"date-parts":[[2000,11,1]]},"DOI":"10.1021\/ic000532g","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T05:56:09Z","timestamp":1027662969000},"page":"5469-5474","source":"Crossref","is-referenced-by-count":14,"title":["Studies on the Mechanism of the Reaction between Coenzyme B<sub>12<\/sub> and Cyanide:\u2009 Direct <sup>1<\/sup>H NMR Spectroscopic Evidence for a (\u03b2-5\u2018-Deoxyadenosyl)(\u03b1-cyano)cobalamin Intermediate"],"prefix":"10.1021","volume":"39","author":[{"given":"Nicola E.","family":"Brasch","sequence":"first","affiliation":[{"name":"Research School of Chemistry, Australian National University, Canberra ACT 0200, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecca J.","family":"Haupt","sequence":"additional","affiliation":[{"name":"Research School of Chemistry, Australian National University, Canberra ACT 0200, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"316","published-online":{"date-parts":[[2000,11,4]]},"reference":[{"key":"ic000532gb00001\/ic000532gb00001_1","doi-asserted-by":"crossref","first-page":"3216","DOI":"10.1021\/ic961312z","volume":"36","author":"Brasch N. E.","year":"1997","journal-title":"Inorg. Chem."},{"key":"ic000532gb00002\/ic000532gb00002_1","doi-asserted-by":"crossref","first-page":"1016","DOI":"10.1021\/ja00368a017","volume":"104","author":"Reenstra W. W.","year":"1982","journal-title":"J. Am. Chem. Soc."},{"key":"ic000532gb00002\/ic000532gb00002_2","first-page":"951","volume":"35","author":"Reenstra W. W.","year":"1976","journal-title":"Fed. Proc., Fed. Am. Soc. Exptl. Biol."},{"key":"ic000532gb00003\/ic000532gb00003_1","volume-title":"Inorganic Chemistry of Vitamin B12","author":"Pratt J. M.","year":"1972"},{"key":"ic000532gb00004\/ic000532gb00004_1","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1016\/S0022-328X(00)86570-5","volume":"61","author":"Rudakova I. P.","year":"1973","journal-title":"J. Organomet. Chem."},{"key":"ic000532gb00004\/ic000532gb00004_2","doi-asserted-by":"crossref","first-page":"2309","DOI":"10.1016\/S0040-4039(01)96847-9","volume":"25","author":"Yurkevich A. M.","year":"1971","journal-title":"Tetrahedron Lett."},{"key":"ic000532gb00004\/ic000532gb00004_3","first-page":"2523","volume":"43","author":"Rudakova I. P.","year":"1973","journal-title":"Zh. Obshch. Khim."},{"key":"ic000532gb00005\/ic000532gb00005_1","doi-asserted-by":"crossref","first-page":"4608","DOI":"10.1039\/jr9620004608","author":"Johnson A. W.","year":"1962","journal-title":"J. Chem. Soc. A"},{"key":"ic000532gb00006\/ic000532gb00006_1","doi-asserted-by":"crossref","first-page":"3641","DOI":"10.1016\/S0021-9258(18)97192-9","volume":"240","author":"Hogenkamp H. P. C.","year":"1965","journal-title":"J. Biol. Chem."},{"key":"ic000532gb00007\/ic000532gb00007_1","doi-asserted-by":"crossref","first-page":"11142","DOI":"10.1021\/ja954110v","volume":"118","author":"Garr C. D.","year":"1996","journal-title":"J. Am. Chem. Soc."},{"key":"ic000532gb00008\/ic000532gb00008_1","doi-asserted-by":"crossref","first-page":"1697","DOI":"10.1021\/ic980608x","volume":"38","author":"Sirovatka J. M.","year":"1999","journal-title":"Inorg. Chem."},{"key":"ic000532gb00009\/ic000532gb00009_1","first-page":"82","volume-title":"Proceedings of the 2nd European Symposium on Vitamin B12 and Intrinsic Factor","author":"For","year":"1961"},{"key":"ic000532gb00010\/ic000532gb00010_1","doi-asserted-by":"crossref","first-page":"4891","DOI":"10.1021\/ic970295q","volume":"36","author":"Brasch N. E.","year":"1997","journal-title":"Inorg. Chem."},{"key":"ic000532gb00011\/ic000532gb00011_1","doi-asserted-by":"crossref","first-page":"6485","DOI":"10.1039\/jr9650006485","author":"Hayward G. C.","year":"1965","journal-title":"J. Chem. Soc."},{"key":"ic000532gb00011\/ic000532gb00011_2","doi-asserted-by":"crossref","first-page":"1022","DOI":"10.1021\/ja00707a047","volume":"92","author":"Schrauzer G. N.","year":"1970","journal-title":"J. Am. Chem. Soc."},{"key":"ic000532gb00011\/ic000532gb00011_3","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1111\/j.1749-6632.1964.tb45031.x","volume":"112","author":"Hogenkamp H. P. C.","year":"1964","journal-title":"Ann. N.Y. Acad. Sci."},{"key":"ic000532gb00011\/ic000532gb00011_4","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1016\/S0021-9258(18)69550-X","volume":"235","author":"Barker H. A.","year":"1960","journal-title":"J. Biol. Chem."},{"key":"ic000532gb00011\/ic000532gb00011_5","doi-asserted-by":"crossref","first-page":"1911","DOI":"10.1016\/S0021-9258(18)91283-4","volume":"239","author":"Hogenkamp H. P. C.","year":"1964","journal-title":"J. Biol. Chem."},{"key":"ic000532gb00012\/ic000532gb00012_1","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1002\/recl.19921110906","volume":"111","author":"Gerards L. E. H.","year":"1992","journal-title":"Recl. Trav. Chim. Pays-Bas"},{"key":"ic000532gb00012\/ic000532gb00012_2","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1002\/recl.19941130303","volume":"113","author":"Gerards L. E. H.","year":"1994","journal-title":"Recl. Trav. Chim. Pays-Bas"},{"key":"ic000532gb00013\/ic000532gb00013_1","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/S0162-0134(97)10008-3","volume":"69","author":"Fleming P. E.","year":"1998","journal-title":"J. Inorg. Biochem."},{"key":"ic000532gb00014\/ic000532gb00014_1","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/S0162-0134(99)00018-5","volume":"73","author":"Brasch N. E.","year":"1999","journal-title":"J. Inorg. Biochem."},{"key":"ic000532gb00015\/ic000532gb00015_1","doi-asserted-by":"crossref","first-page":"4285","DOI":"10.1021\/ja00275a008","volume":"108","author":"For","year":"1986","journal-title":"J. Am. Chem. Soc."},{"key":"ic000532gb00017\/ic000532gb00017_1","unstructured":"1H NMR spectra of the Co\u2212C homolytic cleavage products 5\u2018-deoxyadenosine and 8,5\u2018-anhydroadenosine in 92% DMF-d7\/8% D2O have signals in the aromatic region at 8.41, 8.25, 6.04 (d), and 8.19 and 6.12 ppm, respectively (0.50 M (TBA)ClO4, 25.0 \u00b0C). Addition of ca. 5.0 \u00d7 10-2M (TBA)CN to these solutions changed the chemical shifts by \u22640.02 ppm."},{"key":"ic000532gb00021\/ic000532gb00021_1","unstructured":"The1H NMR spectrum of a freshly prepared solution of AdoCbl (5.1 \u00d7 10-3M) in ca. 9.3 \u00d7 10-3M (TBA)CN (92% DMF-d7\/8% D2O, 25.0 \u00b0C,I= 0.50 M ((TBA)ClO4)) was recorded. SinceK= 2.2 \u00d7 102M-1, then under these conditions (\u03b2-Ado)(\u03b1-CN)Cbl-\/AdoCbl is ca. 1.4:1. The chemical shifts were found to be between those expected for AdoCbl and (\u03b2-Ado)(\u03b1-CN)Cbl-; e.g., C10 5.84 (cf. C10(AdoCbl) = 6.14, C10((\u03b2-Ado)(\u03b1-CN)Cbl-) = 5.65)."},{"key":"ic000532gb00022\/ic000532gb00022_1","unstructured":"It was not possible to carry out1H NMR experiments at [CN-] < 2.00 \u00d7 10-2M, since at least 2 \u00d7 10-3M AdoCbl is required in solution to obtain an acceptable signal-to-noise ratio. CN-concentrations were at least 10 times greater than this to ensure pseudo-first-order conditions."},{"key":"ic000532gb00023\/ic000532gb00023_1","doi-asserted-by":"crossref","first-page":"7894","DOI":"10.1021\/ja00337a043","volume":"106","author":"From","year":"1984","journal-title":"J. Am. Chem. Soc."},{"key":"ic000532gb00024\/ic000532gb00024_1","volume-title":"isosbestic points","author":"Upon"},{"key":"ic000532gb00025\/ic000532gb00025_1","first-page":"219","volume-title":"J. H. In Chemical Kinetics and Reaction Mechanisms","author":"The","year":"1995","edition":"2"},{"key":"ic000532gb00026\/ic000532gb00026_1","doi-asserted-by":"crossref","first-page":"11142","DOI":"10.1021\/ja954110v","volume":"118","author":"Garr C. D.","year":"1996","journal-title":"J. Am. Chem. Soc."},{"key":"ic000532gb00027\/ic000532gb00027_1","doi-asserted-by":"crossref","first-page":"2386","DOI":"10.1021\/ic990058i","volume":"38","author":"Jensen M. P.","year":"1999","journal-title":"Inorg. Chem."},{"key":"ic000532gb00028\/ic000532gb00028_1","doi-asserted-by":"crossref","first-page":"2181","DOI":"10.1021\/ja982340e","volume":"121","author":"Jensen M. P.","year":"1999","journal-title":"J. Am. Chem. Soc."},{"key":"ic000532gb00030\/ic000532gb00030_1","doi-asserted-by":"crossref","first-page":"2095","DOI":"10.1139\/v87-347","volume":"65","author":"Nome F.","year":"1987","journal-title":"Can. J. Chem."},{"key":"ic000532gb00030\/ic000532gb00030_2","doi-asserted-by":"crossref","first-page":"2277","DOI":"10.1021\/ja00242a008","volume":"109","author":"Brown K. L.","year":"1987","journal-title":"J. Am. Chem. Soc."},{"key":"ic000532gb00031\/ic000532gb00031_1","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1039\/qr9621600163","volume":"16","author":"Parker A. J.","year":"1962","journal-title":"Rev., Chem. Soc."},{"key":"ic000532gb00032\/ic000532gb00032_1","doi-asserted-by":"crossref","first-page":"3057","DOI":"10.1021\/ja963380q","volume":"119","author":"Sirovatka J. M.","year":"1997","journal-title":"J. Am. Chem. Soc."},{"key":"ic000532gb00033\/ic000532gb00033_1","first-page":"209","volume-title":"Organic Reactivity:\u2009 Physical and Biological Aspects","author":"Kr\u00e4utler","year":"1995"},{"key":"ic000532gb00034\/ic000532gb00034_1","first-page":"44","volume":"335","author":"Bernhauer K.","year":"1961","journal-title":"Biochem. Z."}],"container-title":["Inorganic Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/ic000532g","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,21]],"date-time":"2021-09-21T12:51:39Z","timestamp":1632228699000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/doi\/10.1021\/ic000532g"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,11,1]]},"references-count":38,"journal-issue":{"issue":"24","published-print":{"date-parts":[[2000,11,1]]}},"alternative-id":["10.1021\/ic000532g"],"URL":"https:\/\/doi.org\/10.1021\/ic000532g","relation":{},"ISSN":["0020-1669","1520-510X"],"issn-type":[{"value":"0020-1669","type":"print"},{"value":"1520-510X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,11,1]]}}}