{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T05:34:13Z","timestamp":1789191253093,"version":"build-2803163510"},"reference-count":52,"publisher":"American Chemical Society (ACS)","issue":"23","license":[{"start":{"date-parts":[[2017,12,31]],"date-time":"2017-12-31T00:00:00Z","timestamp":1514678400000},"content-version":"vor","delay-in-days":2042,"URL":"https:\/\/pubs.acs.org\/page\/policy\/authorchoice_termsofuse.html"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,6,13]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Temperature is a key parameter in physiological processes, and probes able to detect small changes in local temperature are necessary for accurate and quantitative physical descriptions of cellular events. Several have recently emerged that offer excellent temperature sensitivity, spatial resolution, or cellular compatibility, but it has been challenging to realize all of these properties in a single construct. Here, we introduce a luminescent nanocrystal-based sensor that achieves this with a 2.4% change\/\u00b0C ratiometric response over physiological temperatures in aqueous buffers, with a precision of at least 0.2 \u00b0C. Thermoresponsive dual emission is conferred by a Fo\u0308rster resonant energy transfer (FRET) process between CdSe\u2013CdS quantum dot\u2013quantum rods (QD\u2013QRs) as donors and cyanine dyes as acceptors, which are conjugated to QD\u2013QRs using an amphiphilic polymer coating. The nanothermometers were delivered to live cells using a pH-responsive cationic polymer colloid, which served to both improve uptake and release nanocrystals from endosomal confinement. Within cells, they showed an unexpected enhancement in their temperature response and sensitivity, highlighting the need to calibrate these and similar probes within the cell.<\/jats:p>","DOI":"10.1021\/ja302290e","type":"journal-article","created":{"date-parts":[[2012,5,29]],"date-time":"2012-05-29T18:05:59Z","timestamp":1338314759000},"page":"9565-9568","source":"Crossref","is-referenced-by-count":176,"title":["Dual-Emitting Quantum\nDot\/Quantum Rod-Based Nanothermometers\nwith Enhanced Response and Sensitivity in Live Cells"],"prefix":"10.1021","volume":"134","author":[{"given":"Aaron E.","family":"Albers","sequence":"first","affiliation":[{"name":"Lawrence Berkeley National Laboratory The Molecular Foundry, , Berkeley, California 94720, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emory M.","family":"Chan","sequence":"additional","affiliation":[{"name":"Lawrence Berkeley National Laboratory The Molecular Foundry, , Berkeley, California 94720, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick M.","family":"McBride","sequence":"additional","affiliation":[{"name":"Lawrence Berkeley National Laboratory The Molecular Foundry, , Berkeley, California 94720, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline M.","family":"Ajo-Franklin","sequence":"additional","affiliation":[{"name":"Lawrence Berkeley National Laboratory The Molecular Foundry, , Berkeley, California 94720, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce E.","family":"Cohen","sequence":"additional","affiliation":[{"name":"Lawrence Berkeley National Laboratory The Molecular Foundry, , Berkeley, California 94720, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brett A.","family":"Helms","sequence":"additional","affiliation":[{"name":"Lawrence Berkeley National Laboratory The Molecular Foundry, , Berkeley, California 94720, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2012,5,29]]},"reference":[{"key":"2026090217012033700_cit1","doi-asserted-by":"crossref","first-page":"3048","DOI":"10.1021\/jp0665832","volume":"111","author":"Marcus","year":"2007","journal-title":"J. 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