{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T21:55:19Z","timestamp":1791842119848,"version":"4.3.4"},"reference-count":49,"publisher":"American Chemical Society (ACS)","issue":"9","license":[{"start":{"date-parts":[[2015,2,12]],"date-time":"2015-02-12T00:00:00Z","timestamp":1423699200000},"content-version":"vor","delay-in-days":365,"URL":"https:\/\/pubs.acs.org\/page\/policy\/authorchoice_termsofuse.html"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["GM078553"],"award-info":[{"award-number":["GM078553"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31270855"],"award-info":[{"award-number":["31270855"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31300075"],"award-info":[{"award-number":["31300075"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,3,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Cytochrome P450 enzymes are capable of catalyzing a great variety of synthetically useful reactions such as selective C\u2013H functionalization. Surrogate redox partners are widely used for reconstitution of P450 activity based on the assumption that the choice of these auxiliary proteins or their mode of action does not affect the type and selectivity of reactions catalyzed by P450s. Herein, we present an exceptional example to challenge this postulate. MycG, a multifunctional biosynthetic P450 monooxygenase responsible for hydroxylation and epoxidation of 16-membered ring macrolide mycinamicins, is shown to catalyze the unnatural N-demethylation(s) of a range of mycinamicin substrates when partnered with the free Rhodococcus reductase domain RhFRED or the engineered Rhodococcus-spinach hybrid reductase RhFRED-Fdx. By contrast, MycG fused with the RhFRED or RhFRED-Fdx reductase domain mediates only physiological oxidations. This finding highlights the larger potential role of variant redox partner protein\u2013protein interactions in modulating the catalytic activity of P450 enzymes.<\/jats:p>","DOI":"10.1021\/ja4130302","type":"journal-article","created":{"date-parts":[[2014,2,12]],"date-time":"2014-02-12T10:26:30Z","timestamp":1392200790000},"page":"3640-3646","source":"Crossref","is-referenced-by-count":82,"title":["New Reactions\nand Products Resulting from Alternative\nInteractions between the P450 Enzyme and Redox Partners"],"prefix":"10.1021","volume":"136","author":[{"given":"Wei","family":"Zhang","sequence":"first","affiliation":[{"name":"Chinese Academy of Sciences , , , ,","place":["Qingdao, Shandong, China, 266101"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Liu","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , , ,","place":["Qingdao, Shandong, China, 266101"]},{"name":"University of Chinese Academy of Sciences , ,","place":["Beijing, China, 100049"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyong","family":"Yan","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , , ,","place":["Qingdao, Shandong, China, 266101"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaona","family":"Cao","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , , ,","place":["Qingdao, Shandong, China, 266101"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fali","family":"Bai","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , , ,","place":["Qingdao, Shandong, China, 266101"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Yang","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , , ,","place":["Qingdao, Shandong, China, 266101"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaohua","family":"Huang","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , , ,","place":["Qingdao, Shandong, China, 266101"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lishan","family":"Yao","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , , ,","place":["Qingdao, Shandong, China, 266101"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yojiro","family":"Anzai","sequence":"additional","affiliation":[{"name":"Toho University , , ,","place":["Funabashi, Chiba, Japan, 274-8510"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumio","family":"Kato","sequence":"additional","affiliation":[{"name":"Toho University , , ,","place":["Funabashi, Chiba, Japan, 274-8510"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larissa M.","family":"Podust","sequence":"additional","affiliation":[{"name":"University of California , , , ,","place":["San Francisco, California, United States, 94158"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David H.","family":"Sherman","sequence":"additional","affiliation":[{"name":"University of Michigan , , , ,","place":["Ann Arbor, Michigan, United States, 48109"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengying","family":"Li","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , , ,","place":["Qingdao, Shandong, China, 266101"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2014,2,12]]},"reference":[{"key":"2026090123062559000_cit1","doi-asserted-by":"crossref","DOI":"10.1007\/b139087","volume-title":"Cytochrome\nP450: Structure, Mechanism, and Biochemistry","author":"Ortiz de Montellano","year":"2005","edition":"3"},{"key":"2026090123062559000_cit2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1146\/annurev.pharmtox.45.120403.100030","volume":"45","author":"Coon","year":"2005","journal-title":"Annu. Rev. Pharmacol.\nToxicol."},{"key":"2026090123062559000_cit3","doi-asserted-by":"crossref","first-page":"1251","DOI":"10.1039\/c2np20020a","volume":"29","author":"Podust","year":"2012","journal-title":"Nat. Prod. Rep."},{"key":"2026090123062559000_cit4","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1021\/tx0002583","volume":"14","author":"Guengerich","year":"2001","journal-title":"Chem. Res. Toxicol."},{"key":"2026090123062559000_cit5","doi-asserted-by":"crossref","first-page":"17065","DOI":"10.1074\/jbc.R113.462275","volume":"288","author":"Guengerich","year":"2013","journal-title":"J. Biol. Chem."},{"key":"2026090123062559000_cit6","doi-asserted-by":"crossref","first-page":"1718","DOI":"10.1128\/AEM.02580-10","volume":"77","author":"Rude","year":"2011","journal-title":"Appl. Environ. Microbiol."},{"key":"2026090123062559000_cit7","doi-asserted-by":"crossref","first-page":"814","DOI":"10.1038\/nchembio.1048","volume":"8","author":"Barry","year":"2012","journal-title":"Nat. Chem. Biol."},{"key":"2026090123062559000_cit8","doi-asserted-by":"crossref","first-page":"36711","DOI":"10.1074\/jbc.M109.064683","volume":"284","author":"Zhao","year":"2009","journal-title":"J. Biol. Chem."},{"key":"2026090123062559000_cit9","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1126\/science.1231434","volume":"339","author":"Coelho","year":"2013","journal-title":"Science"},{"key":"2026090123062559000_cit10","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1039\/B604190F","volume":"24","author":"Munro","year":"2007","journal-title":"Nat. Prod. Rep."},{"key":"2026090123062559000_cit11","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/j.bbagen.2006.07.017","volume":"1770","author":"Hannemann","year":"2007","journal-title":"Biochim. Biophys.\nActa"},{"key":"2026090123062559000_cit12","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/j.bbagen.2006.08.018","volume":"1770","author":"Munro","year":"2007","journal-title":"Biochim. Biophys.\nActa"},{"key":"2026090123062559000_cit13","doi-asserted-by":"crossref","first-page":"3898","DOI":"10.1128\/JB.184.14.3898-3908.2002","volume":"184","author":"Roberts","year":"2002","journal-title":"J. Bacteriol."},{"key":"2026090123062559000_cit14","doi-asserted-by":"crossref","first-page":"12940","DOI":"10.1021\/ja075842d","volume":"129","author":"Li","year":"2007","journal-title":"J. Am. Chem. Soc."},{"key":"2026090123062559000_cit15","doi-asserted-by":"crossref","first-page":"18463","DOI":"10.1073\/pnas.0907203106","volume":"106","author":"Li","year":"2009","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"2026090123062559000_cit16","doi-asserted-by":"crossref","first-page":"628","DOI":"10.1038\/nchem.1087","volume":"3","author":"Carlson","year":"2011","journal-title":"Nat. Chem."},{"key":"2026090123062559000_cit17","doi-asserted-by":"crossref","first-page":"17486","DOI":"10.1074\/jbc.M700863200","volume":"282","author":"Chun","year":"2007","journal-title":"J. Biol. Chem."},{"key":"2026090123062559000_cit18","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1039\/b003180l","volume":"116","author":"Sadeghi","year":"2000","journal-title":"Faraday Discuss"},{"key":"2026090123062559000_cit19","doi-asserted-by":"crossref","first-page":"950","DOI":"10.1016\/j.chembiol.2008.07.014","volume":"15","author":"Anzai","year":"2008","journal-title":"Chem. Biol."},{"key":"2026090123062559000_cit20","doi-asserted-by":"crossref","first-page":"26289","DOI":"10.1074\/jbc.M605478200","volume":"281","author":"Sherman","year":"2006","journal-title":"J. Biol. Chem."},{"key":"2026090123062559000_cit21","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1007\/s00253-010-3062-9","volume":"90","author":"Harada","year":"2011","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"2026090123062559000_cit22","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1073\/pnas.2435922100","volume":"101","author":"Lei","year":"2004","journal-title":"Proc. Natl. Acad.\nSci. U.S.A."},{"key":"2026090123062559000_cit23","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1042\/bj3080901","volume":"308","author":"Lee-Robichaud","year":"1995","journal-title":"Biochem. J."},{"key":"2026090123062559000_cit24","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1677\/joe.1.06375","volume":"187","author":"Akhtar","year":"2005","journal-title":"J. Endocrinol."},{"key":"2026090123062559000_cit25","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1038\/nature10743","volume":"482","author":"DeVore","year":"2012","journal-title":"Nature"},{"key":"2026090123062559000_cit26","doi-asserted-by":"crossref","first-page":"17008","DOI":"10.1074\/jbc.M113.468926","volume":"288","author":"Estrada","year":"2013","journal-title":"J. Biol. Chem."},{"key":"2026090123062559000_cit27","doi-asserted-by":"crossref","first-page":"17036","DOI":"10.1074\/jbc.273.27.17036","volume":"273","author":"Bridges","year":"1998","journal-title":"J. Biol. Chem."},{"key":"2026090123062559000_cit28","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1111\/j.1574-6968.2003.tb11509.x","volume":"218","author":"Anzai","year":"2003","journal-title":"FEMS Microbiol. Lett."},{"key":"2026090123062559000_cit29","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1007\/BF00302258","volume":"245","author":"Inouye","year":"1994","journal-title":"Mol. Gen. Genet."},{"key":"2026090123062559000_cit30","doi-asserted-by":"crossref","first-page":"364","DOI":"10.7164\/antibiotics.33.364","volume":"33","author":"Satoi","year":"1980","journal-title":"J. Antibiot."},{"key":"2026090123062559000_cit31","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1111\/j.1574-6968.1998.tb13213.x","volume":"167","author":"Takenaka","year":"1998","journal-title":"FEMS Microbiol. Lett."},{"key":"2026090123062559000_cit32","doi-asserted-by":"crossref","first-page":"2478","DOI":"10.1039\/b901716j","volume":"2009","author":"Robin","year":"2009","journal-title":"Chem. Commun."},{"key":"2026090123062559000_cit33","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1002\/cbic.201000104","volume":"11","author":"Sabbadin","year":"2010","journal-title":"ChemBioChem"},{"key":"2026090123062559000_cit34","doi-asserted-by":"crossref","first-page":"1825","DOI":"10.1271\/bbb.90199","volume":"73","author":"Fujita","year":"2009","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"2026090123062559000_cit35","doi-asserted-by":"crossref","first-page":"1494","DOI":"10.3762\/bjoc.7.173","volume":"7","author":"Robin","year":"2011","journal-title":"Beilstein J. Org. Chem."},{"key":"2026090123062559000_cit36","doi-asserted-by":"crossref","first-page":"868","DOI":"10.7164\/antibiotics.38.868","volume":"38","author":"Harada","year":"1985","journal-title":"J.\nAntibiot."},{"key":"2026090123062559000_cit37","doi-asserted-by":"crossref","first-page":"3242","DOI":"10.1021\/ja109590h","volume":"133","author":"Zhang","year":"2011","journal-title":"J. Am. Chem. Soc."},{"key":"2026090123062559000_cit38","doi-asserted-by":"crossref","first-page":"48914","DOI":"10.1074\/jbc.M309630200","volume":"278","author":"Roberts","year":"2003","journal-title":"J. Biol. Chem."},{"key":"2026090123062559000_cit39","doi-asserted-by":"crossref","first-page":"2215","DOI":"10.1016\/j.febslet.2005.03.016","volume":"579","author":"Hunter","year":"2005","journal-title":"FEBS Lett."},{"key":"2026090123062559000_cit40","doi-asserted-by":"crossref","first-page":"2815","DOI":"10.1002\/bit.24960","volume":"110","author":"Ba","year":"2013","journal-title":"Biotechnol. Bioeng."},{"key":"2026090123062559000_cit41","doi-asserted-by":"crossref","first-page":"862","DOI":"10.1021\/ac202787n","volume":"84","author":"Harskamp","year":"2012","journal-title":"Anal. Chem."},{"key":"2026090123062559000_cit42","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1007\/s10529-011-0768-4","volume":"34","author":"Jensen","year":"2012","journal-title":"Biotechnol. Lett."},{"key":"2026090123062559000_cit43","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.1042\/BST0341173","volume":"34","author":"Girvan","year":"2006","journal-title":"Biochem. Soc.\nTrans."},{"key":"2026090123062559000_cit44","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1016\/j.chembiol.2010.05.026","volume":"17","author":"Kells","year":"2010","journal-title":"Chem. Biol."},{"key":"2026090123062559000_cit45","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1111\/j.1574-6968.2010.01899.x","volume":"304","author":"Tsukada","year":"2010","journal-title":"FEMS Microbiol. Lett."},{"key":"2026090123062559000_cit46","doi-asserted-by":"crossref","first-page":"37880","DOI":"10.1074\/jbc.M112.410340","volume":"287","author":"Li","year":"2012","journal-title":"J. Biol. Chem."},{"key":"2026090123062559000_cit47","doi-asserted-by":"crossref","first-page":"6974","DOI":"10.1021\/ja051195j","volume":"127","author":"Wei","year":"2005","journal-title":"J. Am. Chem. Soc."},{"key":"2026090123062559000_cit48","doi-asserted-by":"crossref","first-page":"1227","DOI":"10.1126\/science.1235797","volume":"240","author":"Tripathi","year":"2013","journal-title":"Science"},{"key":"2026090123062559000_cit49","doi-asserted-by":"crossref","first-page":"5964","DOI":"10.1021\/jp101894k","volume":"114","author":"Fishelovitch","year":"2010","journal-title":"J. Phys. Chem. B"}],"container-title":["Journal of\nthe American Chemical Society"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/ja4130302","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/jacsat\/article-pdf\/136\/9\/3640\/8037144\/ja4130302.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/jacsat\/article-pdf\/136\/9\/3640\/8037144\/ja4130302.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T03:09:33Z","timestamp":1788318573000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/jacsat\/article\/136\/9\/3640\/783598\/New-Reactions-and-Products-Resulting-from"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,2,12]]},"references-count":49,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2014,2,12]]},"published-print":{"date-parts":[[2014,3,5]]}},"URL":"https:\/\/doi.org\/10.1021\/ja4130302","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.718276150.793500570","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.718276150.793502259","asserted-by":"object"}]},"ISSN":["0002-7863","1520-5126"],"issn-type":[{"value":"0002-7863","type":"print"},{"value":"1520-5126","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,2,12]]}}}