{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T21:22:18Z","timestamp":1790803338247,"version":"4.1.0"},"reference-count":30,"publisher":"American Chemical Society (ACS)","issue":"2","license":[{"start":{"date-parts":[[2015,12,31]],"date-time":"2015-12-31T00:00:00Z","timestamp":1451520000000},"content-version":"vor","delay-in-days":365,"URL":"https:\/\/pubs.acs.org\/page\/policy\/authorchoice_termsofuse.html"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["GM035906"],"award-info":[{"award-number":["GM035906"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["GM040541"],"award-info":[{"award-number":["GM040541"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000928","name":"Welch Foundation","doi-asserted-by":"publisher","award":["F-1501"],"award-info":[{"award-number":["F-1501"]}],"id":[{"id":"10.13039\/100000928","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003050","name":"Ministry of Economy, Trade and Industry","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/501100003050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,1,21]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Versipelostatin (VST) is an unusual 17-membered macrocyclic polyketide product that contains a spirotetronate skeleton. In this study, the entire VST biosynthetic gene cluster (vst) spanning 108 kb from Streptomyces versipellis 4083-SVS6 was identified by heterologous expression using a bacterial artificial chromosome vector. Here, we demonstrate that an enzyme, VstJ, catalyzes the stereoselective [4+2]-cycloaddition between the conjugated diene and the exocyclic olefin of a newly identified tetronate-containing intermediate to form the spirotetronate skeleton during VST biosynthesis.<\/jats:p>","DOI":"10.1021\/ja510711x","type":"journal-article","created":{"date-parts":[[2014,12,31]],"date-time":"2014-12-31T13:21:27Z","timestamp":1420032087000},"page":"572-575","source":"Crossref","is-referenced-by-count":100,"title":["Biosynthesis\nof Versipelostatin: Identification of\nan Enzyme-Catalyzed [4+2]-Cycloaddition Required for Macrocyclization\nof Spirotetronate-Containing Polyketides"],"prefix":"10.1021","volume":"137","author":[{"given":"Takuya","family":"Hashimoto","sequence":"first","affiliation":[{"name":"The University of Tokyo , , 1-1-1 Yayoi, Bunkyo-ku , ,","place":["Tokyo, Japan, 113-8657"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junko","family":"Hashimoto","sequence":"additional","affiliation":[{"name":"Japan Biological Informatics Consortium , 2-4-7 Aomi, Koto-ku , ,","place":["Tokyo, Japan, 135-0064"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuniko","family":"Teruya","sequence":"additional","affiliation":[{"name":"Okinawa Life Science Research Center 120 , , 5-8 Suzaki, Uruma , ,","place":["Okinawa, Japan, 904-2234"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Hirano","sequence":"additional","affiliation":[{"name":"Okinawa Life Science Research Center 120 , , 5-8 Suzaki, Uruma , ,","place":["Okinawa, Japan, 904-2234"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuo","family":"Shin-ya","sequence":"additional","affiliation":[{"name":"National Institute of Advanced Industrial Science and Technology , 2-4-7 Aomi, Koto-ku , ,","place":["Tokyo, Japan, 135-0064"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haruo","family":"Ikeda","sequence":"additional","affiliation":[{"name":"Kitasato University , , 1-15-1 Kitasato, Minami-ku , , ,","place":["Sagamihara, Kanagawa, Japan, 252-0373"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hung-wen","family":"Liu","sequence":"additional","affiliation":[{"name":"University of Texas at Austin , , , ,","place":["Austin, Texas, United States, 78712"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Nishiyama","sequence":"additional","affiliation":[{"name":"The University of Tokyo , , 1-1-1 Yayoi, Bunkyo-ku , ,","place":["Tokyo, Japan, 113-8657"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomohisa","family":"Kuzuyama","sequence":"additional","affiliation":[{"name":"The University of Tokyo , , 1-1-1 Yayoi, Bunkyo-ku , ,","place":["Tokyo, Japan, 113-8657"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2014,12,31]]},"reference":[{"key":"2026090118203092800_cit1","doi-asserted-by":"crossref","first-page":"4688","DOI":"10.1002\/anie.200806121","volume":"48","author":"Hertweck","year":"2009","journal-title":"Angew. 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