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Vesicle impact electrochemical cytometry (VIEC) was used to determine the number of catecholamine molecules expelled from single vesicles onto an inner-wall carbon surface, where the duration of transmitter release was quantified and correlated to the vesicle size all in the same nanotip. This in turn allowed us to both size and count molecules for vesicles in a living cell. Here, small and sharp open CNPs were employed to carry out intracellular VIEC with minimal invasion and high sensitivity. Our findings with VIEC reveal that the vesicular content increases with vesicle size. The release kinetics of vesicular transmitters and dense core size have the same relation with the vesicle size, implying that the vesicular dense core size determines the speed of each release event. This direct correlation unravels one of the complexities of exocytosis.<\/jats:p>","DOI":"10.1021\/jacs.0c07169","type":"journal-article","created":{"date-parts":[[2020,9,16]],"date-time":"2020-09-16T13:52:18Z","timestamp":1600264338000},"page":"16910-16914","source":"Crossref","is-referenced-by-count":54,"title":["Correlating\nMolecule Count and Release Kinetics with\nVesicular Size Using Open Carbon Nanopipettes"],"prefix":"10.1021","volume":"142","author":[{"given":"Keke","family":"Hu","sequence":"first","affiliation":[{"name":"University of Gothenburg , , Kemiva\u0308gen 10 , ,","place":["Gothenburg, Sweden, 41296"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6094-982X","authenticated-orcid":true,"given":"Rui","family":"Jia","sequence":"additional","affiliation":[{"name":"Queens College-CUNY , , , ,","place":["Flushing, New York, United States, 11367"]},{"name":"The Graduate Center of the City University of New York , , ,","place":["New York, New York, United States, 10016"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7085-893X","authenticated-orcid":true,"given":"Amir","family":"Hatamie","sequence":"additional","affiliation":[{"name":"University of Gothenburg , , Kemiva\u0308gen 10 , ,","place":["Gothenburg, Sweden, 41296"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kim Long","family":"Le Vo","sequence":"additional","affiliation":[{"name":"University of Gothenburg , , Kemiva\u0308gen 10 , ,","place":["Gothenburg, Sweden, 41296"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael V.","family":"Mirkin","sequence":"additional","affiliation":[{"name":"Queens College-CUNY , , , ,","place":["Flushing, New York, United States, 11367"]},{"name":"The Graduate Center of the City University of New York , , ,","place":["New York, New York, United States, 10016"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2084-0133","authenticated-orcid":true,"given":"Andrew G.","family":"Ewing","sequence":"additional","affiliation":[{"name":"University of Gothenburg , , Kemiva\u0308gen 10 , ,","place":["Gothenburg, Sweden, 41296"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2020,9,16]]},"reference":[{"key":"2026090313552826500_cit1","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1016\/0968-0004(91)90012-K","article-title":"The granin-(chromogranin\/secretogranin)\nfamily","volume":"16","author":"Huttner","year":"1991","journal-title":"Trends Biochem. 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