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However, the utility of this functionality in chemical synthesis of peptides and proteins remains unexplored. We report, for the first time that PdII complexes can mediate the efficient removal of various succinimide derivatives in aqueous conditions. Succinimide removal by PdII was applied for the synthesis of two ubiquitin activity-based probes (Ub-ABPs) employing solid phase chemical ligation (SPCL). SPCL was achieved through a sequential three segment ligation on a polymer support via a maleimide anchor. The obtained probes successfully formed the expected covalent complexes with deubiquitinating enzymes (DUBs) USP2 and USP7, highlighting the use of our new method for efficient preparation of unique synthetic proteins. Importantly, we demonstrate the advantages of our newly developed method for the protection and deprotection of native cysteine with a succinimide group in a peptide fragment derived from thioredoxin-1 (Trx-1) obtained via intein based expression to enable ligation\/desulfurization and subsequent disulfide bond formation in a one-pot process.<\/jats:p>","DOI":"10.1021\/jacs.0c07663","type":"journal-article","created":{"date-parts":[[2020,11,2]],"date-time":"2020-11-02T14:44:30Z","timestamp":1604328270000},"page":"19558-19569","source":"Crossref","is-referenced-by-count":46,"title":["On-Demand\nDetachment of Succinimides on Cysteine to\nFacilitate (Semi)Synthesis of Challenging Proteins"],"prefix":"10.1021","volume":"142","author":[{"given":"Ganga B.","family":"Vamisetti","sequence":"first","affiliation":[{"name":"Technion-Israel Institute of Technology , , ,","place":["Haifa, Israel, 3200008"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gandhesiri","family":"Satish","sequence":"additional","affiliation":[{"name":"Technion-Israel Institute of Technology , , ,","place":["Haifa, Israel, 3200008"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prasad","family":"Sulkshane","sequence":"additional","affiliation":[{"name":"Technion-Israel Institute of Technology , , ,","place":["Haifa, Israel, 3200008"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guy","family":"Mann","sequence":"additional","affiliation":[{"name":"Technion-Israel Institute of Technology , , ,","place":["Haifa, Israel, 3200008"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael H.","family":"Glickman","sequence":"additional","affiliation":[{"name":"Technion-Israel Institute of Technology , , ,","place":["Haifa, Israel, 3200008"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8745-2250","authenticated-orcid":true,"given":"Ashraf","family":"Brik","sequence":"additional","affiliation":[{"name":"Technion-Israel Institute of Technology , , ,","place":["Haifa, Israel, 3200008"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2020,11,2]]},"reference":[{"key":"2026090114540072400_cit1a","doi-asserted-by":"publisher","first-page":"6389","DOI":"10.3390\/molecules20046389","article-title":"Exploiting\nProtected Maleimides to Modify Oligonucleotides, Peptides and Peptide\nNucleic Acids","volume":"20","author":"Paris","year":"2015","journal-title":"Molecules"},{"key":"2026090114540072400_cit1b","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1002\/chem.201803174","article-title":"Bioconjugation with Maleimides: A Useful Tool for Chemical\nBiology","volume":"25","author":"Ravasco","year":"2019","journal-title":"Chem. - Eur. 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Soc."},{"key":"2026090114540072400_cit15b","doi-asserted-by":"publisher","first-page":"8108","DOI":"10.1002\/anie.201603169","article-title":"Efficient Palladium-Assisted One-Pot Deprotection of (Acetamidomethyl)\nCysteine Following Native Chemical Ligation and\/or Desulfurization\nTo Expedite Chemical Protein Synthesis","volume":"55","author":"Maity","year":"2016","journal-title":"Angew.\nChem., Int. Ed."},{"key":"2026090114540072400_cit15c","doi-asserted-by":"publisher","first-page":"4061","DOI":"10.1039\/C8OB00890F","article-title":"Palladium mediated\ndeallylation in fully aqueous conditions for native chemical ligation\nat aspartic and glutamic acid sites","volume":"16","author":"Jbara","year":"2018","journal-title":"Org. Biomol.\nChem."},{"key":"2026090114540072400_cit15d","doi-asserted-by":"publisher","first-page":"3154","DOI":"10.1038\/s41467-018-05628-0","article-title":"Palladium prompted on-demand cysteine chemistry for the synthesis\nof challenging and uniquely modified proteins","volume":"9","author":"Jbara","year":"2018","journal-title":"Nat. Commun."},{"key":"2026090114540072400_cit16","doi-asserted-by":"publisher","first-page":"5729","DOI":"10.1002\/anie.201900988","article-title":"Palladium-Mediated Direct Disulfide\nBond Formation in Proteins Containing S-Acetamidomethyl-cysteine under\nAqueous Conditions","volume":"58","author":"Laps","year":"2019","journal-title":"Angew. Chem., Int. Ed."},{"key":"2026090114540072400_cit17","doi-asserted-by":"publisher","first-page":"3026","DOI":"10.1021\/acs.orglett.6b01442","article-title":"Palladium-Assisted\nRemoval of a Solubilizing Tag from a Cys Side\nChain To Facilitate Peptide and Protein Synthesis","volume":"18","author":"Maity","year":"2016","journal-title":"Org. Lett."},{"key":"2026090114540072400_cit18a","doi-asserted-by":"publisher","first-page":"14851","DOI":"10.1002\/chem.201603676","article-title":"Palladium-Assisted Cleavage of Peptides and Proteins Containing a\nBackbone with Thiazolidine Linkage","volume":"22","author":"Jbara","year":"2016","journal-title":"Chem. -\nEur. J."},{"key":"2026090114540072400_cit18b","doi-asserted-by":"publisher","first-page":"13540","DOI":"10.1002\/anie.201906545","article-title":"Palladium-Mediated\nCleavage of Proteins with Thiazolidine-Modified Backbone in Live Cells","volume":"58","author":"Mann","year":"2019","journal-title":"Angew. Chem., Int. 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