{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T21:10:18Z","timestamp":1787001018261,"version":"build-2736575974"},"reference-count":34,"publisher":"American Chemical Society (ACS)","issue":"19","license":[{"start":{"date-parts":[[2022,4,21]],"date-time":"2022-04-21T00:00:00Z","timestamp":1650499200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000011","name":"Howard Hughes Medical Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000011","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["T32NS105595"],"award-info":[{"award-number":["T32NS105595"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005188","name":"Tobacco-Related Disease Research Program","doi-asserted-by":"publisher","award":["27FT-0022"],"award-info":[{"award-number":["27FT-0022"]}],"id":[{"id":"10.13039\/100005188","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005188","name":"Tobacco-Related Disease Research Program","doi-asserted-by":"publisher","award":["27IP-0057"],"award-info":[{"award-number":["27IP-0057"]}],"id":[{"id":"10.13039\/100005188","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005188","name":"Tobacco-Related Disease Research Program","doi-asserted-by":"publisher","award":["T29IR0455"],"award-info":[{"award-number":["T29IR0455"]}],"id":[{"id":"10.13039\/100005188","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000026","name":"National Institute on Drug Abuse","doi-asserted-by":"publisher","award":["DA043829"],"award-info":[{"award-number":["DA043829"]}],"id":[{"id":"10.13039\/100000026","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000026","name":"National Institute on Drug Abuse","doi-asserted-by":"publisher","award":["DA049140"],"award-info":[{"award-number":["DA049140"]}],"id":[{"id":"10.13039\/100000026","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["GM-123582"],"award-info":[{"award-number":["GM-123582"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["GM-7616"],"award-info":[{"award-number":["GM-7616"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Am. Chem. Soc."],"published-print":{"date-parts":[[2022,5,18]]},"DOI":"10.1021\/jacs.2c02323","type":"journal-article","created":{"date-parts":[[2022,4,21]],"date-time":"2022-04-21T16:55:16Z","timestamp":1650560116000},"page":"8480-8486","source":"Crossref","is-referenced-by-count":10,"title":["Three Mutations Convert the Selectivity of a Protein Sensor from Nicotinic Agonists to S-Methadone for Use in Cells, Organelles, and Biofluids"],"prefix":"10.1021","volume":"144","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1041-914X","authenticated-orcid":true,"given":"Anand K.","family":"Muthusamy","sequence":"first","affiliation":[{"name":"Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California 91106, United States"},{"name":"Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91106, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlene H.","family":"Kim","sequence":"additional","affiliation":[{"name":"Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California 91106, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott C.","family":"Virgil","sequence":"additional","affiliation":[{"name":"Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91106, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hailey J.","family":"Knox","sequence":"additional","affiliation":[{"name":"Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91106, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2294-4515","authenticated-orcid":true,"given":"Jonathan S.","family":"Marvin","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, Virginia 20147, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron L.","family":"Nichols","sequence":"additional","affiliation":[{"name":"Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California 91106, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce N.","family":"Cohen","sequence":"additional","affiliation":[{"name":"Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California 91106, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1464-2461","authenticated-orcid":true,"given":"Dennis A.","family":"Dougherty","sequence":"additional","affiliation":[{"name":"Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91106, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Loren L.","family":"Looger","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, University of California, San Diego, San Diego, California 92093, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henry A.","family":"Lester","sequence":"additional","affiliation":[{"name":"Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California 91106, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2022,4,21]]},"reference":[{"key":"ref1\/cit1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drugalcdep.2020.108321"},{"key":"ref2\/cit2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1965.03090080008002"},{"key":"ref3\/cit3","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1971.03180200055012"},{"key":"ref4\/cit4","volume-title":"Handbook of Methadone Prescribing and Buprenorphine Therapy","author":"Cruciani R.","edition":"1"},{"key":"ref5\/cit5","doi-asserted-by":"publisher","DOI":"10.2165\/00003088-200241140-00003"},{"key":"ref6\/cit6","doi-asserted-by":"publisher","DOI":"10.1007\/BF03256276"},{"key":"ref7\/cit7","doi-asserted-by":"publisher","DOI":"10.1007\/BF00542117"},{"key":"ref8\/cit8","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2125.2000.00272.x"},{"key":"ref9\/cit9","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2012.05.020"},{"key":"ref10\/cit10","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2013.12.001"},{"key":"ref11\/cit11","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2018.04.021"},{"key":"ref12\/cit12","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2020.116006"},{"key":"ref13\/cit13","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/bky068"},{"key":"ref14\/cit14","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2016.02.050"},{"key":"ref15\/cit15","doi-asserted-by":"publisher","DOI":"10.1039\/D0AY01374A"},{"key":"ref16\/cit16","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2021.130284"},{"key":"ref17\/cit17","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.28909"},{"key":"ref18\/cit18","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2333"},{"key":"ref19\/cit19","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.201812201"},{"key":"ref20\/cit20","doi-asserted-by":"publisher","DOI":"10.1101\/2020.02.07.939504"},{"key":"ref21\/cit21","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1398"},{"key":"ref22\/cit22","doi-asserted-by":"publisher","DOI":"10.1021\/ja01192a065"},{"key":"ref23\/cit23","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2019.00499"},{"key":"ref24\/cit24","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(80)90585-8"},{"key":"ref25\/cit25","doi-asserted-by":"publisher","DOI":"10.2165\/11537850-000000000-00000"},{"key":"ref26\/cit26","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.280.6219.972"},{"key":"ref27\/cit27","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.74648"},{"key":"ref28\/cit28","doi-asserted-by":"publisher","DOI":"10.1098\/rsif.2019.0217"},{"key":"ref29\/cit29","doi-asserted-by":"publisher","DOI":"10.11613\/BM.2015.018"},{"key":"ref30\/cit30","doi-asserted-by":"publisher","DOI":"10.3109\/07853890.2011.579150"},{"key":"ref31\/cit31","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(74)90174-9"},{"key":"ref32\/cit32","doi-asserted-by":"publisher","DOI":"10.1038\/s41386-021-01168-2"},{"key":"ref33\/cit33","doi-asserted-by":"publisher","DOI":"10.1001\/jamanetworkopen.2021.5731"},{"key":"ref34\/cit34","doi-asserted-by":"publisher","DOI":"10.1038\/s41386-019-0501-x"}],"container-title":["Journal of the American Chemical Society"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/jacs.2c02323","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/jacs.2c02323","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,27]],"date-time":"2023-04-27T20:28:11Z","timestamp":1682627291000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/doi\/10.1021\/jacs.2c02323"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,21]]},"references-count":34,"journal-issue":{"issue":"19","published-print":{"date-parts":[[2022,5,18]]}},"alternative-id":["10.1021\/jacs.2c02323"],"URL":"https:\/\/doi.org\/10.1021\/jacs.2c02323","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2022.02.24.481226","asserted-by":"object"}]},"ISSN":["0002-7863","1520-5126"],"issn-type":[{"value":"0002-7863","type":"print"},{"value":"1520-5126","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,21]]}}}