{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T19:25:12Z","timestamp":1793042712256,"version":"4.5.0"},"reference-count":53,"publisher":"American Chemical Society (ACS)","issue":"26","license":[{"start":{"date-parts":[[2015,6,30]],"date-time":"2015-06-30T00:00:00Z","timestamp":1435622400000},"content-version":"vor","delay-in-days":25,"URL":"https:\/\/pubs.acs.org\/page\/policy\/authorchoice_ccby_termsofuse.html"}],"funder":[{"DOI":"10.13039\/100004431","name":"European Commission Directorate-General for Research and Innovation","doi-asserted-by":"publisher","award":["PIIF-GA-2012-328085 RPSII"],"award-info":[{"award-number":["PIIF-GA-2012-328085 RPSII"]}],"id":[{"id":"10.13039\/100004431","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000266","name":"Engineering and Physical Sciences Research Council","doi-asserted-by":"publisher","award":["EP\/H00338X\/2"],"award-info":[{"award-number":["EP\/H00338X\/2"]}],"id":[{"id":"10.13039\/501100000266","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/K010220\/1"],"award-info":[{"award-number":["BB\/K010220\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000288","name":"Royal Society","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/501100000288","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004794","name":"Centre National de la Recherche Scientifique","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/501100004794","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/na","name":"Commissariat \u00e0 l\u2019\u00e9nergie atomique","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,7,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>In natural photosynthesis, light is used for the production of chemical energy carriers to fuel biological activity. The re-engineering of natural photosynthetic pathways can provide inspiration for sustainable fuel production and insights for understanding the process itself. Here, we employ a semiartificial approach to study photobiological water splitting via a pathway unavailable to nature: the direct coupling of the water oxidation enzyme, photosystem II, to the H2 evolving enzyme, hydrogenase. Essential to this approach is the integration of the isolated enzymes into the artificial circuit of a photoelectrochemical cell. We therefore developed a tailor-made hierarchically structured indium\u2013tin oxide electrode that gives rise to the excellent integration of both photosystem II and hydrogenase for performing the anodic and cathodic half-reactions, respectively. When connected together with the aid of an applied bias, the semiartificial cell demonstrated quantitative electron flow from photosystem II to the hydrogenase with the production of H2 and O2 being in the expected two-to-one ratio and a light-to-hydrogen conversion efficiency of 5.4% under low-intensity red-light irradiation. We thereby demonstrate efficient light-driven water splitting using a pathway inaccessible to biology and report on a widely applicable in vitro platform for the controlled coupling of enzymatic redox processes to meaningfully study photocatalytic reactions.<\/jats:p>","DOI":"10.1021\/jacs.5b03737","type":"journal-article","created":{"date-parts":[[2015,6,5]],"date-time":"2015-06-05T12:41:07Z","timestamp":1433508067000},"page":"8541-8549","source":"Crossref","is-referenced-by-count":274,"title":["Wiring\nof Photosystem II to Hydrogenase for Photoelectrochemical\nWater Splitting"],"prefix":"10.1021","volume":"137","author":[{"given":"Dirk","family":"Mersch","sequence":"first","affiliation":[{"name":"University of Cambridge , , Lensfield Road , ,","place":["Cambridge, U.K., CB2 1EW"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chong-Yong","family":"Lee","sequence":"additional","affiliation":[{"name":"University of Cambridge , , Lensfield Road , ,","place":["Cambridge, U.K., CB2 1EW"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenny Zhenqi","family":"Zhang","sequence":"additional","affiliation":[{"name":"University of Cambridge , , Lensfield Road , ,","place":["Cambridge, U.K., CB2 1EW"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharina","family":"Brinkert","sequence":"additional","affiliation":[{"name":"Imperial College London , , ,","place":["London, U.K., SW7 2AZ"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan C.","family":"Fontecilla-Camps","sequence":"additional","affiliation":[{"name":"Universite\u0301 Grenoble Alpes Metalloproteins Unit, Institut de Biologie Structurale, CEA, CNRS, , ,","place":["Grenoble, France, 38044"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. William","family":"Rutherford","sequence":"additional","affiliation":[{"name":"Imperial College London , , ,","place":["London, U.K., SW7 2AZ"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erwin","family":"Reisner","sequence":"additional","affiliation":[{"name":"University of Cambridge , , Lensfield Road , ,","place":["Cambridge, U.K., CB2 1EW"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2015,6,5]]},"reference":[{"key":"2026090108200362200_cit1","doi-asserted-by":"crossref","first-page":"6446","DOI":"10.1021\/cr1002326","volume":"110","author":"Walter","year":"2010","journal-title":"Chem. Rev."},{"key":"2026090108200362200_cit2","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1126\/science.1209816","volume":"334","author":"Reece","year":"2011","journal-title":"Science"},{"key":"2026090108200362200_cit3","doi-asserted-by":"crossref","first-page":"842","DOI":"10.1038\/nmat3684","volume":"12","author":"Warren","year":"2013","journal-title":"Nat. Mater."},{"key":"2026090108200362200_cit4","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1038\/nrm1525","volume":"5","author":"Nelson","year":"2004","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2026090108200362200_cit5","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1126\/science.1200165","volume":"332","author":"Blankenship","year":"2011","journal-title":"Science"},{"key":"2026090108200362200_cit6","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.plaphy.2008.01.001","volume":"46","author":"Andersson","year":"2008","journal-title":"Plant Physiol. Biochem."},{"key":"2026090108200362200_cit7","doi-asserted-by":"crossref","first-page":"6485","DOI":"10.1039\/C4CS00031E","volume":"43","author":"Kato","year":"2014","journal-title":"Chem. Soc. Rev."},{"key":"2026090108200362200_cit8","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1146\/annurev-biochem-070511-100425","volume":"82","author":"Vinyard","year":"2013","journal-title":"Annu. Rev. Biochem."},{"key":"2026090108200362200_cit9","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.bbabio.2011.07.004","volume":"1817","author":"Grundmeier","year":"2012","journal-title":"Biochim.\nBiophys. Acta, Bioenerg."},{"key":"2026090108200362200_cit10","doi-asserted-by":"crossref","first-page":"4081","DOI":"10.1021\/cr4005814","volume":"114","author":"Lubitz","year":"2014","journal-title":"Chem. Rev."},{"key":"2026090108200362200_cit11","doi-asserted-by":"crossref","first-page":"866","DOI":"10.1039\/b201337a","author":"Jones","year":"2002","journal-title":"Chem. Commun."},{"key":"2026090108200362200_cit12","doi-asserted-by":"crossref","first-page":"5531","DOI":"10.1039\/C1EE02514G","volume":"5","author":"Melis","year":"2012","journal-title":"Energy Environ. Sci."},{"key":"2026090108200362200_cit13","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1007\/s11120-014-9991-x","volume":"123","author":"Dubini","year":"2015","journal-title":"Photosynth. Res."},{"key":"2026090108200362200_cit14","doi-asserted-by":"crossref","first-page":"7223","DOI":"10.1073\/pnas.1220645110","volume":"110","author":"Volgusheva","year":"2013","journal-title":"Proc. Natl. Acad.\nSci. U. S. A."},{"key":"2026090108200362200_cit15","doi-asserted-by":"crossref","first-page":"2516","DOI":"10.1002\/anie.201200218","volume":"51","author":"Michel","year":"2012","journal-title":"Angew. Chem., Int.\nEd."},{"key":"2026090108200362200_cit16","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1038\/nature09913","volume":"473","author":"Umena","year":"2011","journal-title":"Nature"},{"key":"2026090108200362200_cit17","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1016\/S0969-2126(99)80072-0","volume":"7","author":"Garcin","year":"1999","journal-title":"Structure"},{"key":"2026090108200362200_cit18","doi-asserted-by":"crossref","first-page":"2367","DOI":"10.1039\/c2sc20042b","volume":"3","author":"Liu","year":"2012","journal-title":"Chem. Sci."},{"key":"2026090108200362200_cit19","doi-asserted-by":"crossref","first-page":"153102","DOI":"10.1103\/PhysRevB.72.153102","volume":"72","author":"Koenderink","year":"2005","journal-title":"Phys. Rev. B"},{"key":"2026090108200362200_cit20","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1002\/elan.200804191","volume":"20","author":"Badura","year":"2008","journal-title":"Electroanalysis"},{"key":"2026090108200362200_cit21","doi-asserted-by":"crossref","first-page":"8332","DOI":"10.1021\/ja301488d","volume":"134","author":"Kato","year":"2012","journal-title":"J. Am. Chem. Soc."},{"key":"2026090108200362200_cit22","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1039\/B801144N","volume":"38","author":"Armstrong","year":"2009","journal-title":"Chem. Soc. Rev."},{"key":"2026090108200362200_cit23","doi-asserted-by":"crossref","first-page":"11106","DOI":"10.1021\/ja8027668","volume":"130","author":"Goldet","year":"2008","journal-title":"J. Am. Chem. Soc."},{"key":"2026090108200362200_cit24","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1038\/35013024","volume":"405","author":"Blanco","year":"2000","journal-title":"Nature"},{"key":"2026090108200362200_cit25","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1126\/science.282.5390.897","volume":"282","author":"Zakhidov","year":"1998","journal-title":"Science"},{"key":"2026090108200362200_cit26","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1364\/JOSAB.10.000283","volume":"10","author":"Yablonovitch","year":"1993","journal-title":"J. Opt. Soc. Am.\nB"},{"key":"2026090108200362200_cit27","doi-asserted-by":"crossref","first-page":"023116","DOI":"10.1063\/1.2757130","volume":"91","author":"Diguna","year":"2007","journal-title":"Appl. Phys. Lett."},{"key":"2026090108200362200_cit28","doi-asserted-by":"crossref","first-page":"14065","DOI":"10.1021\/la302913j","volume":"28","author":"Schaming","year":"2012","journal-title":"Langmuir"},{"key":"2026090108200362200_cit29","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.electacta.2014.05.046","volume":"140","author":"Liu","year":"2014","journal-title":"Electrochim. Acta"},{"key":"2026090108200362200_cit30","doi-asserted-by":"crossref","first-page":"2984","DOI":"10.1021\/nn2000492","volume":"5","author":"Arsenault","year":"2011","journal-title":"ACS Nano"},{"key":"2026090108200362200_cit31","doi-asserted-by":"crossref","first-page":"6834","DOI":"10.1021\/ja301193s","volume":"134","author":"Renault","year":"2012","journal-title":"J. Am. Chem. Soc."},{"key":"2026090108200362200_cit32","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1093\/oxfordjournals.pcp.a029510","volume":"40","author":"Sugiura","year":"1999","journal-title":"Plant\nCell Physiol."},{"key":"2026090108200362200_cit33","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1007\/s11120-008-9345-7","volume":"98","author":"Renger","year":"2008","journal-title":"Photosynth. Res."},{"key":"2026090108200362200_cit34","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.cbpa.2015.01.001","volume":"25","author":"Bachmeier","year":"2015","journal-title":"Curr. Opin. Chem.\nBiol."},{"key":"2026090108200362200_cit35","doi-asserted-by":"crossref","first-page":"13410","DOI":"10.1021\/ja803657d","volume":"130","author":"Parkin","year":"2008","journal-title":"J. Am. Chem. Soc."},{"key":"2026090108200362200_cit36","doi-asserted-by":"crossref","first-page":"12313","DOI":"10.1002\/anie.201306214","volume":"52","author":"Sakai","year":"2013","journal-title":"Angew. Chem., Int.\nEd."},{"key":"2026090108200362200_cit37","doi-asserted-by":"crossref","first-page":"4366","DOI":"10.1021\/cr050191u","volume":"107","author":"Vincent","year":"2007","journal-title":"Chem. Rev."},{"key":"2026090108200362200_cit38","doi-asserted-by":"crossref","first-page":"18457","DOI":"10.1021\/ja907923r","volume":"131","author":"Reisner","year":"2009","journal-title":"J. Am. Chem. Soc."},{"key":"2026090108200362200_cit39","doi-asserted-by":"crossref","first-page":"12749","DOI":"10.1002\/anie.201207448","volume":"51","author":"Muresan","year":"2012","journal-title":"Angew. Chem., Int.\nEd."},{"key":"2026090108200362200_cit40","doi-asserted-by":"crossref","first-page":"1366","DOI":"10.1039\/C3CP54631D","volume":"16","author":"de Poulpiquet","year":"2014","journal-title":"Phys. Chem. Chem. Phys."},{"key":"2026090108200362200_cit41","doi-asserted-by":"crossref","first-page":"1015","DOI":"10.1039\/c2sc01103d","volume":"3","author":"Krishnan","year":"2012","journal-title":"Chem. Sci."},{"key":"2026090108200362200_cit42","doi-asserted-by":"crossref","first-page":"2439","DOI":"10.1021\/cr0680639","volume":"108","author":"Cracknell","year":"2008","journal-title":"Chem. Rev."},{"key":"2026090108200362200_cit43","doi-asserted-by":"crossref","first-page":"2015","DOI":"10.1021\/ja077691k","volume":"130","author":"Hambourger","year":"2008","journal-title":"J. Am. Chem. Soc."},{"key":"2026090108200362200_cit44","doi-asserted-by":"crossref","first-page":"16008","DOI":"10.1021\/ja0554312","volume":"127","author":"Ru\u0308diger","year":"2005","journal-title":"J. Am. Chem. Soc."},{"key":"2026090108200362200_cit45","doi-asserted-by":"crossref","first-page":"4299","DOI":"10.1021\/ja104785e","volume":"133","author":"Svedruz\u030cic\u0301","year":"2011","journal-title":"J. Am. Chem. Soc."},{"key":"2026090108200362200_cit46","doi-asserted-by":"crossref","first-page":"13260","DOI":"10.1021\/ja2049226","volume":"133","author":"Ulas","year":"2011","journal-title":"J. Am. Chem. Soc."},{"key":"2026090108200362200_cit47","doi-asserted-by":"crossref","first-page":"16951","DOI":"10.1073\/pnas.0504499102","volume":"102","author":"Vincent","year":"2005","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"2026090108200362200_cit48","doi-asserted-by":"crossref","first-page":"14154","DOI":"10.1021\/ja905797w","volume":"131","author":"Lazarus","year":"2009","journal-title":"J. Am. Chem. Soc."},{"key":"2026090108200362200_cit49","doi-asserted-by":"crossref","first-page":"14233","DOI":"10.1002\/anie.201303671","volume":"52","author":"Kothe","year":"2013","journal-title":"Angew. Chem., Int. Ed."},{"key":"2026090108200362200_cit50","doi-asserted-by":"crossref","first-page":"4316","DOI":"10.1021\/ja413001p","volume":"136","author":"Wilker","year":"2014","journal-title":"J. Am. Chem. Soc."},{"key":"2026090108200362200_cit51","doi-asserted-by":"crossref","first-page":"11538","DOI":"10.1002\/anie.201406811","volume":"53","author":"Caputo","year":"2014","journal-title":"Angew. Chem., Int. Ed."},{"key":"2026090108200362200_cit52","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1016\/0006-291X(78)90896-3","volume":"82","author":"Hatchikian","year":"1978","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2026090108200362200_cit53","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1016\/j.bbabio.2010.10.019","volume":"1807","author":"Sedoud","year":"2011","journal-title":"Biochim. Biophys.\nActa, Bioenerg."}],"container-title":["Journal of the American Chemical Society"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/jacs.5b03737","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/jacsat\/article-pdf\/137\/26\/8541\/67431778\/jacs.5b03737.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/jacsat\/article-pdf\/137\/26\/8541\/67431778\/jacs.5b03737.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T12:20:16Z","timestamp":1788265216000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/jacsat\/article\/137\/26\/8541\/979630\/Wiring-of-Photosystem-II-to-Hydrogenase-for"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,6,5]]},"references-count":53,"journal-issue":{"issue":"26","published-online":{"date-parts":[[2015,6,5]]},"published-print":{"date-parts":[[2015,7,8]]}},"URL":"https:\/\/doi.org\/10.1021\/jacs.5b03737","relation":{},"ISSN":["0002-7863","1520-5126"],"issn-type":[{"value":"0002-7863","type":"print"},{"value":"1520-5126","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,6,5]]}}}