{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T12:46:59Z","timestamp":1790858819028,"version":"4.1.0"},"reference-count":94,"publisher":"American Chemical Society (ACS)","issue":"3","funder":[{"DOI":"10.13039\/100000165","name":"Division of Chemistry","doi-asserted-by":"publisher","award":["1152804"],"award-info":[{"award-number":["1152804"]}],"id":[{"id":"10.13039\/100000165","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["5R01GM102205"],"award-info":[{"award-number":["5R01GM102205"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Am. Chem. Soc."],"published-print":{"date-parts":[[2016,1,27]]},"DOI":"10.1021\/jacs.5b12209","type":"journal-article","created":{"date-parts":[[2016,1,6]],"date-time":"2016-01-06T16:17:05Z","timestamp":1452097025000},"page":"1046-1056","source":"Crossref","is-referenced-by-count":127,"title":["Catalytic Promiscuity of Ancestral Esterases and Hydroxynitrile Lyases"],"prefix":"10.1021","volume":"138","author":[{"given":"Titu","family":"Devamani","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Molecular Biology & Biophysics and The Biotechnology Institute, University of Minnesota, 1479 Gortner Avenue, Saint Paul, Minnesota 55108, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alissa M.","family":"Rauwerdink","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Molecular Biology & Biophysics and The Biotechnology Institute, University of Minnesota, 1479 Gortner Avenue, Saint Paul, Minnesota 55108, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Lunzer","sequence":"additional","affiliation":[{"name":"Department of Ecology, Evolution & Behavior and The Biotechnology Institute, University of Minnesota, 1479 Gortner Avenue, Saint Paul, Minnesota 55108, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bryan J.","family":"Jones","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Molecular Biology & Biophysics and The Biotechnology Institute, University of Minnesota, 1479 Gortner Avenue, Saint Paul, Minnesota 55108, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna L.","family":"Mooney","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Molecular Biology & Biophysics and The Biotechnology Institute, University of Minnesota, 1479 Gortner Avenue, Saint Paul, Minnesota 55108, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maxilmilien Alaric O.","family":"Tan","sequence":"additional","affiliation":[{"name":"Department\rof Chemistry, University of Minnesota, 220 Pleasant Street, Minneapolis, Minnesota 55455, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi-Jun","family":"Zhang","sequence":"additional","affiliation":[{"name":"School\rof Biotechnology, East China University of Science, Technology, Meilong Road 130, Shanghai 200237, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian-He","family":"Xu","sequence":"additional","affiliation":[{"name":"School\rof Biotechnology, East China University of Science, Technology, Meilong Road 130, Shanghai 200237, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antony M.","family":"Dean","sequence":"additional","affiliation":[{"name":"Department of Ecology, Evolution & Behavior and The Biotechnology Institute, University of Minnesota, 1479 Gortner Avenue, Saint Paul, Minnesota 55108, United States"},{"name":"Institute\rof Ecology and Evolution, Sun Yat-sen University, No.135, Xinggang West Road, Guangzhou, 510275, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romas J.","family":"Kazlauskas","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Molecular Biology & Biophysics and The Biotechnology Institute, University of Minnesota, 1479 Gortner Avenue, Saint Paul, Minnesota 55108, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2016,1,15]]},"reference":[{"key":"ref1\/cit1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-5521(99)80033-7"},{"key":"ref2\/cit2","doi-asserted-by":"publisher","DOI":"10.1002\/ejoc.201001664"},{"key":"ref3\/cit3","doi-asserted-by":"publisher","DOI":"10.1021\/bi00855a005"},{"key":"ref4\/cit4","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200460416"},{"key":"ref5\/cit5","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(05)81264-1"},{"key":"ref6\/cit6","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1286"},{"key":"ref7\/cit7","doi-asserted-by":"publisher","DOI":"10.1016\/0010-406X(69)91293-6"},{"key":"ref8\/cit8","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5193(74)80035-4"},{"key":"ref9\/cit9","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.30.100176.002205"},{"key":"ref10\/cit10","doi-asserted-by":"publisher","DOI":"10.1126\/science.1217411"},{"key":"ref11\/cit11","doi-asserted-by":"publisher","DOI":"10.1038\/nrg1324"},{"key":"ref12\/cit12","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3540"},{"key":"ref13\/cit13","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1001446"},{"key":"ref14\/cit14","doi-asserted-by":"publisher","DOI":"10.1021\/ja311630a"},{"key":"ref15\/cit15","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1019605109"},{"key":"ref16\/cit16","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaa1823"},{"key":"ref17\/cit17","doi-asserted-by":"publisher","DOI":"10.1104\/pp.94.2.401"},{"key":"ref18\/cit18","doi-asserted-by":"crossref","unstructured":"Gasteiger, E.; Hoogland, C.; Gattiker, A.; Duvaud, S.; Wilkins, M. R.; Appel, R. D.; Bairoch, A.InThe Proteomics Protocols Handbook;Walker, J. G., Ed.Humana Press:Totowa, NJ, 2005; p571.","DOI":"10.1385\/1-59259-890-0:571"},{"key":"ref19\/cit19","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook J.","year":"1989","edition":"2"},{"key":"ref20\/cit20","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh340"},{"key":"ref21\/cit21","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msp259"},{"key":"ref22\/cit22","first-page":"406","volume":"4","author":"Saitou N.","year":"1987","journal-title":"Mol. Biol. Evol."},{"key":"ref23\/cit23","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150181505"},{"key":"ref24\/cit24","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btl446"},{"key":"ref25\/cit25","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msn067"},{"key":"ref26\/cit26","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/8.3.275"},{"key":"ref27\/cit27","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a026201"},{"key":"ref28\/cit28","doi-asserted-by":"crossref","first-page":"1641","DOI":"10.1093\/genetics\/141.4.1641","volume":"141","author":"Yang Z.","year":"1995","journal-title":"Genetics"},{"key":"ref29\/cit29","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst197"},{"key":"ref30\/cit30","doi-asserted-by":"publisher","DOI":"10.1002\/cctc.201000147"},{"key":"ref31\/cit31","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-983897"},{"key":"ref32\/cit32","doi-asserted-by":"publisher","DOI":"10.1002\/app.34512"},{"key":"ref33\/cit33","doi-asserted-by":"publisher","DOI":"10.1021\/ja01519a081"},{"key":"ref34\/cit34","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201402081"},{"key":"ref35\/cit35","first-page":"281","author":"Wolfsie J. H.","year":"1959","journal-title":"J. Occup. Med."},{"key":"ref36\/cit36","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90565-3"},{"key":"ref37\/cit37","doi-asserted-by":"publisher","DOI":"10.1039\/b607863j"},{"key":"ref38\/cit38","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2011.03.011"},{"key":"ref39\/cit39","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200463006"},{"key":"ref40\/cit40","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-3765(19981102)4:11<2324::AID-CHEM2324>3.3.CO;2-9"},{"key":"ref41\/cit41","doi-asserted-by":"publisher","DOI":"10.1039\/p29930002317"},{"key":"ref42\/cit42","doi-asserted-by":"publisher","DOI":"10.1021\/ja108642s"},{"key":"ref43\/cit43","doi-asserted-by":"publisher","DOI":"10.1002\/adsc.201200526"},{"key":"ref44\/cit44","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.21.15701"},{"key":"ref45\/cit45","doi-asserted-by":"publisher","DOI":"10.1002\/1521-3765(20020715)8:14<3211::AID-CHEM3211>3.0.CO;2-G"},{"key":"ref46\/cit46","doi-asserted-by":"publisher","DOI":"10.1214\/ss\/1177011454"},{"key":"ref47\/cit47","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks1154"},{"key":"ref48\/cit48","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201000213"},{"key":"ref49\/cit49","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/5.3.197"},{"key":"ref50\/cit50","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-440X(99)00037-8"},{"key":"ref51\/cit51","doi-asserted-by":"publisher","DOI":"10.2174\/1389203003381405"},{"key":"ref52\/cit52","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msv338"},{"key":"ref53\/cit53","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3054.1996.tb06700.x"},{"key":"ref54\/cit54","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3054.1989.tb02069.x"},{"key":"ref55\/cit55","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M401575200"},{"key":"ref56\/cit56","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1994.1267"},{"key":"ref57\/cit57","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2011.02.004"},{"key":"ref58\/cit58","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0409227102"},{"key":"ref59\/cit59","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1033.2002.02956.x"},{"key":"ref60\/cit60","doi-asserted-by":"publisher","DOI":"10.1007\/s13765-012-2201-7"},{"key":"ref61\/cit61","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200701455"},{"key":"ref62\/cit62","doi-asserted-by":"publisher","DOI":"10.1126\/science.1062249"},{"key":"ref63\/cit63","doi-asserted-by":"publisher","DOI":"10.1038\/nature14907"},{"key":"ref64\/cit64","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1674"},{"key":"ref65\/cit65","doi-asserted-by":"publisher","DOI":"10.1104\/pp.110.157073"},{"key":"ref66\/cit66","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.111.093997"},{"key":"ref67\/cit67","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.87.11.992"},{"key":"ref68\/cit68","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2013.04.019"},{"key":"ref69\/cit69","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1993.1411"},{"key":"ref70\/cit70","doi-asserted-by":"publisher","DOI":"10.1128\/JB.02020-12"},{"key":"ref71\/cit71","doi-asserted-by":"publisher","DOI":"10.1038\/srep08193"},{"key":"ref72\/cit72","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200504230"},{"key":"ref73\/cit73","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-9452(96)04333-6"},{"key":"ref74\/cit74","doi-asserted-by":"publisher","DOI":"10.1002\/anie.199604371"},{"key":"ref75\/cit75","doi-asserted-by":"publisher","DOI":"10.1002\/adsc.200700064"},{"key":"ref76\/cit76","doi-asserted-by":"publisher","DOI":"10.1002\/cctc.201000147"},{"key":"ref77\/cit77","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2011.03.011"},{"key":"ref78\/cit78","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0409227102"},{"key":"ref79\/cit79","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.2000.01136.x"},{"key":"ref80\/cit80","doi-asserted-by":"publisher","DOI":"10.1021\/ja00526a052"},{"key":"ref81\/cit81","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2009.03.010"},{"key":"ref82\/cit82","doi-asserted-by":"publisher","DOI":"10.1023\/A:1024182228057"},{"key":"ref83\/cit83","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2011.03.005"},{"key":"ref84\/cit84","doi-asserted-by":"publisher","DOI":"10.1016\/j.tetlet.2007.01.063"},{"key":"ref85\/cit85","doi-asserted-by":"publisher","DOI":"10.1039\/b803406k"},{"key":"ref86\/cit86","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2010.06.013"},{"key":"ref87\/cit87","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-030409-143718"},{"key":"ref88\/cit88","doi-asserted-by":"publisher","DOI":"10.1126\/science.1157092"},{"key":"ref89\/cit89","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M700868200"},{"key":"ref90\/cit90","doi-asserted-by":"publisher","DOI":"10.1021\/jp800311c"},{"key":"ref91\/cit91","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1015996108"},{"key":"ref92\/cit92","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/21.10.2323"},{"key":"ref93\/cit93","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2009.11.028"},{"key":"ref94\/cit94","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0075272"}],"container-title":["Journal of the American Chemical Society"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/jacs.5b12209","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,26]],"date-time":"2023-04-26T16:11:44Z","timestamp":1682525504000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/doi\/10.1021\/jacs.5b12209"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,1,15]]},"references-count":94,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2016,1,27]]}},"alternative-id":["10.1021\/jacs.5b12209"],"URL":"https:\/\/doi.org\/10.1021\/jacs.5b12209","relation":{},"ISSN":["0002-7863","1520-5126"],"issn-type":[{"value":"0002-7863","type":"print"},{"value":"1520-5126","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,1,15]]}}}