{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T09:37:36Z","timestamp":1776850656456,"version":"3.51.2"},"reference-count":38,"publisher":"American Chemical Society (ACS)","issue":"15","license":[{"start":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T00:00:00Z","timestamp":1775606400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T00:00:00Z","timestamp":1775606400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T00:00:00Z","timestamp":1775606400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Am. Chem. Soc."],"published-print":{"date-parts":[[2026,4,22]]},"DOI":"10.1021\/jacs.5c16773","type":"journal-article","created":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T17:41:34Z","timestamp":1775670094000},"page":"15463-15473","source":"Crossref","is-referenced-by-count":0,"title":["Mechanistic Insights into Chemoselective Reduction of Engineered Cysteine Residues in Antibodies Using Computational Modeling and Rationally Designed Phosphines"],"prefix":"10.1021","volume":"148","author":[{"given":"Manish","family":"Hudlikar","sequence":"first","affiliation":[{"name":"Discovery Chemistry, Merck & Co., Inc., 213 E Grand Ave, South San Francisco, California 94080, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingzhou","family":"Wang","sequence":"additional","affiliation":[{"name":"Discovery Chemistry, Merck & Co., Inc., 213 E Grand Ave, South San Francisco, California 94080, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xingjian","family":"Yu","sequence":"additional","affiliation":[{"name":"Discovery Chemistry, Merck & Co., Inc., 213 E Grand Ave, South San Francisco, California 94080, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Going","sequence":"additional","affiliation":[{"name":"Discovery Biologics and Protein Sciences, Merck & Co., Inc., 213 E Grand Ave, South San Francisco, California 94080, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehabaw Getahun","family":"Derebe","sequence":"additional","affiliation":[{"name":"Discovery Biologics and Protein Sciences, Merck & Co., Inc., 213 E Grand Ave, South San Francisco, California 94080, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4156-4975","authenticated-orcid":true,"given":"Manman","family":"Lu","sequence":"additional","affiliation":[{"name":"Analytical Research and Development, Merck & Co., Inc., 213 E Grand Ave, South San Francisco, California 94080, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-0680-8236","authenticated-orcid":true,"given":"Manoj","family":"Charati","sequence":"additional","affiliation":[{"name":"Discovery Chemistry, Merck & Co., Inc., 213 E Grand Ave, South San Francisco, California 94080, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"316","published-online":{"date-parts":[[2026,4,8]]},"reference":[{"key":"ref1\/cit1","doi-asserted-by":"publisher","DOI":"10.1038\/s43018-023-00516-z"},{"key":"ref2\/cit2","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-024-01963-7"},{"key":"ref3\/cit3","doi-asserted-by":"publisher","DOI":"10.1038\/s41573-023-00709-2"},{"key":"ref4\/cit4","doi-asserted-by":"publisher","DOI":"10.1080\/19420862.2023.2229101"},{"key":"ref5\/cit5","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2022.09.016"},{"key":"ref6\/cit6","doi-asserted-by":"publisher","DOI":"10.1038\/nchem.2415"},{"key":"ref7\/cit7","doi-asserted-by":"publisher","DOI":"10.1080\/2162402X.2017.1395127"},{"key":"ref8\/cit8","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-10-0987"},{"key":"ref9\/cit9","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/3.8.703"},{"key":"ref10\/cit10","doi-asserted-by":"publisher","DOI":"10.1021\/acs.bioconjchem.7b00791"},{"key":"ref11\/cit11","doi-asserted-by":"publisher","DOI":"10.1021\/acs.bioconjchem.7b00785"},{"key":"ref12\/cit12","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1480"},{"key":"ref13\/cit13","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2013.01.010"},{"key":"ref14\/cit14","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-16-0431"},{"key":"ref15\/cit15","doi-asserted-by":"publisher","DOI":"10.1021\/bc400217g"},{"key":"ref16\/cit16","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-17-0742"},{"key":"ref17\/cit17","doi-asserted-by":"publisher","DOI":"10.1208\/s12248-017-0083-7"},{"key":"ref18\/cit18","doi-asserted-by":"publisher","DOI":"10.1021\/acs.bioconjchem.0c00337"},{"key":"ref19\/cit19","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-11344-z"},{"key":"ref20\/cit20","unstructured":"Meyer, D. L.\n                      Selective reduction of proteins\n                      , US 11,667,696 B2, 2019."},{"key":"ref21\/cit21","doi-asserted-by":"publisher","DOI":"10.1021\/acs.bioconjchem.3c00425"},{"key":"ref22\/cit22","doi-asserted-by":"publisher","DOI":"10.1021\/acs.oprd.4c00343"},{"key":"ref23\/cit23","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2010.11.008"},{"key":"ref24\/cit24","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jctc.7b00125"},{"key":"ref25\/cit25","doi-asserted-by":"publisher","DOI":"10.1021\/jo071271w"},{"key":"ref26\/cit26","doi-asserted-by":"publisher","DOI":"10.1021\/acs.bioconjchem.7b00365"},{"key":"ref27\/cit27","doi-asserted-by":"publisher","DOI":"10.1021\/ct100578z"},{"key":"ref28\/cit28","doi-asserted-by":"publisher","DOI":"10.1021\/ja01507a009"},{"key":"ref29\/cit29","doi-asserted-by":"publisher","DOI":"10.1021\/ac60215a035"},{"key":"ref30\/cit30","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.202000092"},{"key":"ref31\/cit31","doi-asserted-by":"publisher","DOI":"10.1021\/ja00419a022"},{"key":"ref32\/cit32","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-23793-7"},{"key":"ref33\/cit33","doi-asserted-by":"publisher","DOI":"10.1080\/19420862.2025.2498162"},{"key":"ref34\/cit34","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2024.116995"},{"key":"ref35\/cit35","doi-asserted-by":"publisher","DOI":"10.1093\/abt\/tbz009"},{"key":"ref36\/cit36","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201608292"},{"key":"ref37\/cit37","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2018.10.043"},{"key":"ref38\/cit38","unstructured":"Coumans, R. G. E.; Spijker, H. J.\n                      Selective reduction of cysteine-engineered antibodies\n                      ; WO 2,017,137,628 A1, 2017."}],"container-title":["Journal of the American Chemical Society"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/jacs.5c16773","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/jacs.5c16773","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T08:37:53Z","timestamp":1776847073000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/doi\/10.1021\/jacs.5c16773"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,8]]},"references-count":38,"journal-issue":{"issue":"15","published-print":{"date-parts":[[2026,4,22]]}},"alternative-id":["10.1021\/jacs.5c16773"],"URL":"https:\/\/doi.org\/10.1021\/jacs.5c16773","relation":{},"ISSN":["0002-7863","1520-5126"],"issn-type":[{"value":"0002-7863","type":"print"},{"value":"1520-5126","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,8]]}}}