{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T15:08:12Z","timestamp":1784732892749,"version":"3.55.0"},"reference-count":37,"publisher":"American Chemical Society (ACS)","issue":"28","license":[{"start":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T00:00:00Z","timestamp":1783468800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100014809","name":"Technology Agency of the Czech Republic","doi-asserted-by":"publisher","award":["ODEEP-EU TH86010001"],"award-info":[{"award-number":["ODEEP-EU TH86010001"]}],"id":[{"id":"10.13039\/100014809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001823","name":"Ministerstvo ?kolstv?, Ml?de?e a Telov?chovy","doi-asserted-by":"publisher","award":["CZ.02.01.01\/00\/22_008\/0004597"],"award-info":[{"award-number":["CZ.02.01.01\/00\/22_008\/0004597"]}],"id":[{"id":"10.13039\/501100001823","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001823","name":"Ministerstvo ?kolstv?, Ml?de?e a Telov?chovy","doi-asserted-by":"publisher","award":["LM2018127"],"award-info":[{"award-number":["LM2018127"]}],"id":[{"id":"10.13039\/501100001823","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001824","name":"Grantov? Agentura Cesk? Republiky","doi-asserted-by":"publisher","award":["22-27695S"],"award-info":[{"award-number":["22-27695S"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001824","name":"Grantov? Agentura Cesk? Republiky","doi-asserted-by":"publisher","award":["25-18181S"],"award-info":[{"award-number":["25-18181S"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007543","name":"Grantov? Agentura, Univerzita Karlova","doi-asserted-by":"publisher","award":["448425"],"award-info":[{"award-number":["448425"]}],"id":[{"id":"10.13039\/100007543","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Am. Chem. Soc."],"published-print":{"date-parts":[[2026,7,22]]},"DOI":"10.1021\/jacs.6c04886","type":"journal-article","created":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T05:58:27Z","timestamp":1783490307000},"page":"29883-29894","source":"Crossref","is-referenced-by-count":0,"title":["An Amphibious Bifunctional Probe for Protein Chemical Cross-Linking"],"prefix":"10.1021","volume":"148","author":[{"given":"Michael","family":"Karp\u00ed\u0161ek","sequence":"first","affiliation":[{"name":"Institute of Microbiology of the Czech Academy of Sciences","place":["Prague, Czech Republic"]},{"name":"Department of Biochemistry, Faculty of Science","place":["Prague, Czech Republic"]},{"name":"Charles University","place":["Prague, Czech Republic"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luk\u00e1\u0161","family":"Fojt\u00edk","sequence":"additional","affiliation":[{"name":"Institute of Microbiology of the Czech Academy of Sciences","place":["Prague, Czech Republic"]},{"name":"Department of Biochemistry, Faculty of Science","place":["Prague, Czech Republic"]},{"name":"Charles University","place":["Prague, Czech Republic"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Fiala","sequence":"additional","affiliation":[{"name":"Institute of Microbiology of the Czech Academy of Sciences","place":["Prague, Czech Republic"]},{"name":"Department of Biochemistry, Faculty of Science","place":["Prague, Czech Republic"]},{"name":"Charles University","place":["Prague, Czech Republic"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vojt\u011bch","family":"Langer","sequence":"additional","affiliation":[{"name":"CF Plus Chemicals","place":["Brno, Czech Republic"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00e1clav","family":"Matou\u0161ek","sequence":"additional","affiliation":[{"name":"CF Plus Chemicals","place":["Brno, Czech Republic"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7569-843X","authenticated-orcid":true,"given":"Zden\u011bk","family":"Kuka\u010dka","sequence":"additional","affiliation":[{"name":"Institute of Microbiology of the Czech Academy of Sciences","place":["Prague, Czech Republic"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8688-529X","authenticated-orcid":true,"given":"Petr","family":"Nov\u00e1k","sequence":"additional","affiliation":[{"name":"Institute of Microbiology of the Czech Academy of Sciences","place":["Prague, Czech Republic"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,7,8]]},"reference":[{"key":"ref1\/cit1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.090099097"},{"key":"ref2\/cit2","doi-asserted-by":"publisher","DOI":"10.1021\/ac302860m"},{"key":"ref3\/cit3","doi-asserted-by":"publisher","DOI":"10.1021\/ac202818m"},{"key":"ref4\/cit4","doi-asserted-by":"publisher","DOI":"10.1111\/febs.15732"},{"key":"ref5\/cit5","doi-asserted-by":"publisher","DOI":"10.1002\/bms.1200201209"},{"key":"ref6\/cit6","doi-asserted-by":"publisher","unstructured":"Rossi, V.; Gaboriaud, C.; Lacroix, M.; Ulrich, J.; Carlos Fontecilla-Camps, J.; Gagnon, J.; Arlaud, G. J. Structure of the catalytic region of human complement protease C1s: study by chemical cross-linking and three-dimensional homology modeling,\n                      Biochemistry\n                      1995; Vol. 34.7311 10.1021\/bi00022a004.https:\/\/pubs.acs.org\/sharingguidelines.","DOI":"10.1021\/bi00022a004"},{"key":"ref7\/cit7","doi-asserted-by":"publisher","DOI":"10.1016\/0022-5193(83)90112-1"},{"key":"ref8\/cit8","doi-asserted-by":"publisher","DOI":"10.1016\/0022-5193(83)90113-3"},{"key":"ref9\/cit9","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2010.10.014"},{"key":"ref10\/cit10","doi-asserted-by":"publisher","DOI":"10.1586\/14789450.2014.960852"},{"key":"ref11\/cit11","doi-asserted-by":"publisher","DOI":"10.1255\/ejms.963"},{"key":"ref12\/cit12","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.885"},{"key":"ref13\/cit13","doi-asserted-by":"publisher","DOI":"10.1110\/ps.052040406"},{"key":"ref14\/cit14","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth752"},{"key":"ref15\/cit15","doi-asserted-by":"publisher","DOI":"10.1002\/jms.1776"},{"key":"ref16\/cit16","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.7b02863"},{"key":"ref17\/cit17","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2008.05.074"},{"key":"ref18\/cit18","doi-asserted-by":"publisher","DOI":"10.1002\/chem.200501052"},{"key":"ref19\/cit19","doi-asserted-by":"publisher","DOI":"10.1002\/chem.201503531"},{"key":"ref20\/cit20","doi-asserted-by":"publisher","DOI":"10.1021\/jo400774u"},{"key":"ref21\/cit21","doi-asserted-by":"publisher","DOI":"10.1002\/chem.201902944"},{"key":"ref22\/cit22","doi-asserted-by":"publisher","DOI":"10.1039\/C9OB02115A"},{"key":"ref23\/cit23","doi-asserted-by":"publisher","DOI":"10.1021\/cr500223h"},{"key":"ref24\/cit24","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.1c07771"},{"key":"ref25\/cit25","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.3c04161"},{"key":"ref26\/cit26","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2020.e05614"},{"key":"ref27\/cit27","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(95)56003-3"},{"key":"ref28\/cit28","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2019104096"},{"key":"ref29\/cit29","doi-asserted-by":"publisher","DOI":"10.1080\/14789450.2025.2492763"},{"key":"ref30\/cit30","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2024.168656"},{"key":"ref31\/cit31","doi-asserted-by":"publisher","DOI":"10.1016\/s0167-4838(97)00064-2"},{"key":"ref32\/cit32","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2016.08.013"},{"key":"ref33\/cit33","doi-asserted-by":"publisher","DOI":"10.1021\/ac302393g"},{"key":"ref34\/cit34","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.1c07771"},{"key":"ref35\/cit35","doi-asserted-by":"publisher","DOI":"10.1039\/d5va00262a"},{"key":"ref36\/cit36","doi-asserted-by":"publisher","DOI":"10.1002\/hlca.200890217"},{"key":"ref37\/cit37","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2020.12.015"}],"container-title":["Journal of the American Chemical Society"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/jacs.6c04886","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/jacs.6c04886","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T14:49:33Z","timestamp":1784731773000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/doi\/10.1021\/jacs.6c04886"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,8]]},"references-count":37,"journal-issue":{"issue":"28","published-print":{"date-parts":[[2026,7,22]]}},"alternative-id":["10.1021\/jacs.6c04886"],"URL":"https:\/\/doi.org\/10.1021\/jacs.6c04886","relation":{},"ISSN":["0002-7863","1520-5126"],"issn-type":[{"value":"0002-7863","type":"print"},{"value":"1520-5126","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,8]]}}}