{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T01:15:27Z","timestamp":1790644527135,"version":"4.1.0"},"reference-count":25,"publisher":"American Chemical Society (ACS)","issue":"33","license":[{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22071032"],"award-info":[{"award-number":["22071032"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["52125308"],"award-info":[{"award-number":["52125308"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["52433014"],"award-info":[{"award-number":["52433014"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["52573073"],"award-info":[{"award-number":["52573073"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["525B1001"],"award-info":[{"award-number":["525B1001"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2024YFC3906700"],"award-info":[{"award-number":["2024YFC3906700"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Enzyme immobilization in solid porous supports often suffers from a trade-off among enzyme loading, mass transport, catalytic activity, and operational stability. Here, we report a liquid-like immobilization platform based on star polyelectrolyte coacervates (SPECs) formed by simple mixing of oppositely charged star polyelectrolytes. The multivalent star topology organizes the coacervate into a compact yet dynamically adaptive network, enabling enzyme loading efficiencies exceeding 97% and order-of-magnitude enhancements in apparent catalytic rates. Moreover, SPEC exhibits topology-enabled ion exclusion that suppresses ion penetration even under high-salinity conditions, thereby stabilizing both the coacervate structure and embedded enzymes. As a result, SPEC maintains high catalytic activity under salinity, pH, temperature, and organic solvent perturbations and supports sustained continuous-flow biocatalysis. The platform is broadly applicable to diverse enzymes, including lipases from multiple biological sources, alkaline phosphatase, and trypsin, thereby establishing SPEC as a general and scalable immobilization strategy for efficient and practical biocatalysis.<\/jats:p>","DOI":"10.1021\/jacs.6c10930","type":"journal-article","created":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T13:01:59Z","timestamp":1786626119000},"page":"36095-36104","source":"Crossref","is-referenced-by-count":2,"title":["Star Polyelectrolyte\nCoacervates Enable Adaptive and\nRobust Enzyme Immobilization for Efficient Biocatalysis"],"prefix":"10.1021","volume":"148","author":[{"given":"Jie","family":"Zhu","sequence":"first","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, People\u2019s Republic of China, 200438"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyuan","family":"Xiao","sequence":"additional","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, People\u2019s Republic of China, 200438"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Wu","sequence":"additional","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, People\u2019s Republic of China, 200438"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinran","family":"Zhao","sequence":"additional","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, People\u2019s Republic of China, 200438"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiyang","family":"Huo","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , ,","place":["Changchun, People\u2019s Republic of China, 130022"]},{"name":"University of Science and Technology of China , , ,","place":["Hefei, People\u2019s Republic of China, 230026"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6357-3039","authenticated-orcid":true,"given":"Zhaoyan","family":"Sun","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , ,","place":["Changchun, People\u2019s Republic of China, 130022"]},{"name":"University of Science and Technology of China , , ,","place":["Hefei, People\u2019s Republic of China, 230026"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3053-7354","authenticated-orcid":true,"given":"Xiayun","family":"Huang","sequence":"additional","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, People\u2019s Republic of China, 200438"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9639-905X","authenticated-orcid":true,"given":"Zhihong","family":"Nie","sequence":"additional","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, People\u2019s Republic of China, 200438"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,13]]},"reference":[{"key":"2026090104501145100_cit1","doi-asserted-by":"publisher","first-page":"381","DOI":"10.1038\/s41586-025-09308-0","article-title":"Repurposing haemoproteins\nfor asymmetric metal-catalysed H atom transfer","volume":"644","author":"Zhang","year":"2025","journal-title":"Nature"},{"key":"2026090104501145100_cit2","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1038\/s41586-023-06027-2","article-title":"Complete integration of carbene-transfer chemistry\ninto biosynthesis","volume":"617","author":"Huang","year":"2023","journal-title":"Nature"},{"key":"2026090104501145100_cit3","doi-asserted-by":"publisher","first-page":"8740","DOI":"10.1021\/acs.chemrev.4c00120","article-title":"Noncanonical\nAmino Acids in Biocatalysis","volume":"124","author":"Birch-Price","year":"2024","journal-title":"Chem. 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