{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T19:22:14Z","timestamp":1772133734381,"version":"3.50.1"},"reference-count":60,"publisher":"American Chemical Society (ACS)","issue":"12","license":[{"start":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T00:00:00Z","timestamp":1763424000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JACS Au"],"published-print":{"date-parts":[[2025,12,22]]},"DOI":"10.1021\/jacsau.5c00950","type":"journal-article","created":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T13:11:27Z","timestamp":1763471487000},"page":"6060-6071","source":"Crossref","is-referenced-by-count":0,"title":["Engineering an Artificial Myxopyronin Derivative with Enhanced Metabolic Stability via Mutasynthesis"],"prefix":"10.1021","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4540-6501","authenticated-orcid":true,"given":"Alexander F.","family":"Kiefer","sequence":"first","affiliation":[{"name":"Helmholtz Institute for Pharmaceutical Research Saarland (HIPS)","place":["Saarbr\u00fccken, Germany"]},{"name":"Helmholtz Centre for Infection Research (HZI), Inhoffenstra\u00dfe 7, 38124, Braunschweig, Germany"},{"name":"German Center for Infection Research (DZIF), Inhoffenstra\u00dfe 7, 38124, Braunschweig, Germany"},{"name":"PharmaScienceHub (PSH)","place":["Saarbr\u00fccken, Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Voltz","sequence":"additional","affiliation":[{"name":"Helmholtz Institute for Pharmaceutical Research Saarland (HIPS)","place":["Saarbr\u00fccken, Germany"]},{"name":"Helmholtz Centre for Infection Research (HZI), Inhoffenstra\u00dfe 7, 38124, Braunschweig, Germany"},{"name":"German Center for Infection Research (DZIF), Inhoffenstra\u00dfe 7, 38124, Braunschweig, Germany"},{"name":"PharmaScienceHub (PSH)","place":["Saarbr\u00fccken, Germany"]},{"name":"Saarland University","place":["Saarbr\u00fccken, Germany"]},{"name":"Department of Pharmacy","place":["Saarbr\u00fccken, Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Domen","family":"Scherzer","sequence":"additional","affiliation":[{"name":"Helmholtz Institute for Pharmaceutical Research Saarland (HIPS)","place":["Saarbr\u00fccken, Germany"]},{"name":"Helmholtz Centre for Infection Research (HZI), Inhoffenstra\u00dfe 7, 38124, Braunschweig, Germany"},{"name":"German Center for Infection Research (DZIF), Inhoffenstra\u00dfe 7, 38124, Braunschweig, Germany"},{"name":"PharmaScienceHub (PSH)","place":["Saarbr\u00fccken, Germany"]},{"name":"Saarland University","place":["Saarbr\u00fccken, Germany"]},{"name":"Department of Pharmacy","place":["Saarbr\u00fccken, Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roman","family":"Reber\u0161ek","sequence":"additional","affiliation":[{"name":"Helmholtz Institute for Pharmaceutical Research Saarland (HIPS)","place":["Saarbr\u00fccken, Germany"]},{"name":"Helmholtz Centre for Infection Research (HZI), Inhoffenstra\u00dfe 7, 38124, Braunschweig, Germany"},{"name":"German Center for Infection Research (DZIF), Inhoffenstra\u00dfe 7, 38124, Braunschweig, Germany"},{"name":"PharmaScienceHub (PSH)","place":["Saarbr\u00fccken, Germany"]},{"name":"Saarland University","place":["Saarbr\u00fccken, Germany"]},{"name":"Department of Pharmacy","place":["Saarbr\u00fccken, Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas M.","family":"Kany","sequence":"additional","affiliation":[{"name":"Helmholtz Institute for Pharmaceutical Research Saarland (HIPS)","place":["Saarbr\u00fccken, Germany"]},{"name":"Helmholtz Centre for Infection Research (HZI), Inhoffenstra\u00dfe 7, 38124, Braunschweig, Germany"},{"name":"German Center for Infection Research (DZIF), Inhoffenstra\u00dfe 7, 38124, Braunschweig, Germany"},{"name":"PharmaScienceHub (PSH)","place":["Saarbr\u00fccken, Germany"]},{"name":"Saarland University","place":["Saarbr\u00fccken, Germany"]},{"name":"Department of Pharmacy","place":["Saarbr\u00fccken, Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gareth","family":"Prosser","sequence":"additional","affiliation":[{"name":"German Center for Infection Research (DZIF), Inhoffenstra\u00dfe 7, 38124, Braunschweig, Germany"},{"name":"Microbial Interface Biology, Research Center Borstel, Leibniz Lung Center, Parkallee 1, 23845 Borstel, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Markus","family":"Neuber","sequence":"additional","affiliation":[{"name":"Helmholtz Institute for Pharmaceutical Research Saarland (HIPS)","place":["Saarbr\u00fccken, Germany"]},{"name":"Helmholtz Centre for Infection Research (HZI), Inhoffenstra\u00dfe 7, 38124, Braunschweig, Germany"},{"name":"German Center for Infection Research (DZIF), Inhoffenstra\u00dfe 7, 38124, Braunschweig, Germany"},{"name":"PharmaScienceHub (PSH)","place":["Saarbr\u00fccken, Germany"]},{"name":"Saarland University","place":["Saarbr\u00fccken, Germany"]},{"name":"Department of Pharmacy","place":["Saarbr\u00fccken, Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6673-4291","authenticated-orcid":true,"given":"Norbert","family":"Reiling","sequence":"additional","affiliation":[{"name":"German Center for Infection Research (DZIF), Inhoffenstra\u00dfe 7, 38124, Braunschweig, Germany"},{"name":"Microbial Interface Biology, Research Center Borstel, Leibniz Lung Center, Parkallee 1, 23845 Borstel, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8734-4663","authenticated-orcid":true,"given":"Anna K. H.","family":"Hirsch","sequence":"additional","affiliation":[{"name":"Helmholtz Institute for Pharmaceutical Research Saarland (HIPS)","place":["Saarbr\u00fccken, Germany"]},{"name":"Helmholtz Centre for Infection Research (HZI), Inhoffenstra\u00dfe 7, 38124, Braunschweig, Germany"},{"name":"German Center for Infection Research (DZIF), Inhoffenstra\u00dfe 7, 38124, Braunschweig, Germany"},{"name":"PharmaScienceHub (PSH)","place":["Saarbr\u00fccken, Germany"]},{"name":"Saarland University","place":["Saarbr\u00fccken, Germany"]},{"name":"Department of Pharmacy","place":["Saarbr\u00fccken, Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1042-5665","authenticated-orcid":true,"given":"Rolf","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Helmholtz Institute for Pharmaceutical Research Saarland (HIPS)","place":["Saarbr\u00fccken, Germany"]},{"name":"Helmholtz Centre for Infection Research (HZI), Inhoffenstra\u00dfe 7, 38124, Braunschweig, Germany"},{"name":"German Center for Infection Research (DZIF), Inhoffenstra\u00dfe 7, 38124, Braunschweig, Germany"},{"name":"PharmaScienceHub (PSH)","place":["Saarbr\u00fccken, Germany"]},{"name":"Saarland University","place":["Saarbr\u00fccken, Germany"]},{"name":"Department of Pharmacy","place":["Saarbr\u00fccken, Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"316","published-online":{"date-parts":[[2025,11,18]]},"reference":[{"key":"ref1\/cit1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(24)01867-1"},{"key":"ref2\/cit2","author":"World Health Organization","year":"2024","journal-title":"WHO bacterial priority pathogens list, 2024:Bacterial pathogens of public health importance to guide research, development Bacterial pathogens of public health importance to guide research, development and strategies to prevent and control antimicrobial resistance"},{"key":"ref3\/cit3","unstructured":"World Health Organization.\n                      Global tuberculosis report 2024\n                      , 2024."},{"key":"ref4\/cit4","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2019.10.008"},{"key":"ref5\/cit5","doi-asserted-by":"publisher","DOI":"10.15252\/embr.202256033"},{"key":"ref6\/cit6","doi-asserted-by":"publisher","DOI":"10.1002\/jlac.198419840605"},{"key":"ref7\/cit7","doi-asserted-by":"publisher","DOI":"10.1002\/jlac.198319831003"},{"key":"ref8\/cit8","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.36.1651"},{"key":"ref9\/cit9","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.38.145"},{"key":"ref10\/cit10","doi-asserted-by":"publisher","DOI":"10.1002\/jlac.198519850418"},{"key":"ref11\/cit11","doi-asserted-by":"publisher","DOI":"10.1039\/D2NP00012A"},{"key":"ref12\/cit12","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2011.07.030"},{"key":"ref13\/cit13","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.09.033"},{"key":"ref14\/cit14","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2009.10.010"},{"key":"ref15\/cit15","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2004.08.045"},{"key":"ref16\/cit16","doi-asserted-by":"publisher","DOI":"10.1021\/jo9721610"},{"key":"ref17\/cit17","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2007.10.017"},{"key":"ref18\/cit18","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201206560"},{"key":"ref19\/cit19","unstructured":"Ebright, R. H. WO2025144774A1: THERAPEUTIC COMPOUNDS AND METHODS, 2025."},{"key":"ref20\/cit20","unstructured":"Ebright, R. H.; Ebright, Y. W. WO2019160875A1:ANTIBACTERIAL AGENTS: SOLUBLE SALTS AND AQUEOUS FORMULATIONS OF PYRONINS, 2019."},{"key":"ref21\/cit21","unstructured":"Ebright, R. H.; Ebright, Y. W.; Shen, J.; Bacci, J.; Hiebel, A.C. WO2013192352A1:ANTIBACTERIAL AGENTS: ARYL MYXOPYRONIN DERIVATIVES, 2013."},{"key":"ref22\/cit22","unstructured":"Ebright, R. H.; Ebright, Y. W. WO2012037508A2:ANTIBACTERIAL AGENTS: HIGH-POTENCY MYXOPYRONIN DERIVATIVES, 2012."},{"key":"ref23\/cit23","unstructured":"Ebright, R. H.; Ebright, Y. W. WO2013142812A1:ANTIBACTERIAL AGENTS: SIDECHAIN-FLUORINATED MYXOPYRONIN DERIVATIVES, 2013."},{"key":"ref24\/cit24","unstructured":"Ebright, R. H.; Ebright, Y. W. WO2019160873A1:ANTIBACTERIAL AGENTS: O-ALKYL-DEUTERATED PYRONINS, 2019."},{"key":"ref25\/cit25","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2007.02.004"},{"key":"ref26\/cit26","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201300289"},{"key":"ref27\/cit27","doi-asserted-by":"publisher","DOI":"10.1039\/C5SC01013F"},{"key":"ref28\/cit28","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201402666"},{"key":"ref29\/cit29","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2017.10.004"},{"key":"ref30\/cit30","doi-asserted-by":"publisher","DOI":"10.1021\/acssynbio.9b00023"},{"key":"ref31\/cit31","doi-asserted-by":"publisher","DOI":"10.1039\/D1NP00067E"},{"key":"ref32\/cit32","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2016.08.014"},{"key":"ref33\/cit33","doi-asserted-by":"publisher","DOI":"10.1021\/ja404134f"},{"key":"ref34\/cit34","doi-asserted-by":"publisher","DOI":"10.1038\/s41557-022-00996-z"},{"key":"ref35\/cit35","doi-asserted-by":"publisher","DOI":"10.1021\/acs.orglett.5b02113"},{"key":"ref36\/cit36","doi-asserted-by":"publisher","DOI":"10.1021\/ja063217n"},{"key":"ref37\/cit37","doi-asserted-by":"publisher","DOI":"10.1021\/ja00838a039"},{"key":"ref38\/cit38","doi-asserted-by":"publisher","DOI":"10.1021\/acs.orglett.0c02190"},{"key":"ref39\/cit39","doi-asserted-by":"publisher","DOI":"10.1039\/C5CS00897B"},{"key":"ref40\/cit40","doi-asserted-by":"publisher","DOI":"10.1039\/a903980e"},{"key":"ref41\/cit41","doi-asserted-by":"publisher","DOI":"10.1021\/ja0471525"},{"key":"ref42\/cit42","doi-asserted-by":"publisher","DOI":"10.1021\/cr0783479"},{"key":"ref43\/cit43","doi-asserted-by":"publisher","DOI":"10.1016\/j.tet.2016.02.031"},{"key":"ref44\/cit44","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-21163-x"},{"key":"ref45\/cit45","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201207690"},{"key":"ref46\/cit46","doi-asserted-by":"publisher","DOI":"10.1007\/s10295-018-2085-6"},{"key":"ref47\/cit47","doi-asserted-by":"publisher","DOI":"10.1039\/C5NP00125K"},{"key":"ref48\/cit48","doi-asserted-by":"publisher","DOI":"10.1038\/s41589-019-0313-7"},{"key":"ref49\/cit49","doi-asserted-by":"publisher","DOI":"10.1016\/j.engmic.2024.100155"},{"key":"ref50\/cit50","doi-asserted-by":"publisher","DOI":"10.1016\/j.bej.2015.10.015"},{"key":"ref51\/cit51","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics12111105"},{"key":"ref52\/cit52","doi-asserted-by":"publisher","DOI":"10.3389\/fitd.2022.983107"},{"key":"ref53\/cit53","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202102831"},{"key":"ref54\/cit54","doi-asserted-by":"publisher","DOI":"10.1016\/j.chempr.2020.11.010"},{"key":"ref55\/cit55","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.05627.x"},{"key":"ref56\/cit56","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2008.10.010"},{"key":"ref57\/cit57","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2008.05.001"},{"key":"ref58\/cit58","doi-asserted-by":"publisher","DOI":"10.1039\/c3np70060g"},{"key":"ref59\/cit59","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2005.10.001"},{"key":"ref60\/cit60","doi-asserted-by":"publisher","DOI":"10.1039\/C6NP00017G"}],"container-title":["JACS Au"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/jacsau.5c00950","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/jacsau.5c00950","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T11:03:55Z","timestamp":1768561435000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/doi\/10.1021\/jacsau.5c00950"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,18]]},"references-count":60,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2025,12,22]]}},"alternative-id":["10.1021\/jacsau.5c00950"],"URL":"https:\/\/doi.org\/10.1021\/jacsau.5c00950","relation":{"has-preprint":[{"id-type":"doi","id":"10.26434\/chemrxiv-2025-q4h0n","asserted-by":"object"}]},"ISSN":["2691-3704","2691-3704"],"issn-type":[{"value":"2691-3704","type":"print"},{"value":"2691-3704","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,18]]}}}