{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T21:32:17Z","timestamp":1791495137490,"version":"4.3.4"},"reference-count":125,"publisher":"American Chemical Society (ACS)","issue":"12","license":[{"start":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T00:00:00Z","timestamp":1762905600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFF1205104"],"award-info":[{"award-number":["2023YFF1205104"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004739","name":"Youth Innovation Promotion Association of the Chinese Academy of Sciences","doi-asserted-by":"publisher","award":["2020282"],"award-info":[{"award-number":["2020282"]}],"id":[{"id":"10.13039\/501100004739","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82130105"],"award-info":[{"award-number":["82130105"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82273766"],"award-info":[{"award-number":["82273766"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["92478204"],"award-info":[{"award-number":["92478204"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Institutes for Drug Discovery and Development, Chinese Academy of Sciences","award":["CASIMM0120225007"],"award-info":[{"award-number":["CASIMM0120225007"]}]},{"name":"SA-SIBS Scholarship Program","award":["CASIMM0120225007"],"award-info":[{"award-number":["CASIMM0120225007"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JACS Au"],"published-print":{"date-parts":[[2025,12,22]]},"DOI":"10.1021\/jacsau.5c01134","type":"journal-article","created":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T01:12:48Z","timestamp":1762996368000},"page":"5866-5887","source":"Crossref","is-referenced-by-count":19,"title":["The Current Toolbox for Covalent Inhibitors: From Hit Identification to Drug Discovery"],"prefix":"10.1021","volume":"5","author":[{"given":"Mengke","family":"You","sequence":"first","affiliation":[{"name":"State Key Laboratory of Drug Research","place":["Shanghai, China"]},{"name":"Shanghai Institute of Materia Medica, Chinese Academy of Sciences","place":["Shanghai, China"]},{"name":"University of Chinese Academy of Sciences","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3685-6268","authenticated-orcid":true,"given":"Hong","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Drug Research","place":["Shanghai, China"]},{"name":"Shanghai Institute of Materia Medica, Chinese Academy of Sciences","place":["Shanghai, China"]},{"name":"University of Chinese Academy of Sciences","place":["Beijing, China"]},{"name":"School of Pharmaceutical Science and Technology","place":["Hangzhou, China"]},{"name":"Hangzhou Institute for Advanced Study, UCAS","place":["Hangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3000-0728","authenticated-orcid":true,"given":"Chunpu","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Drug Research","place":["Shanghai, China"]},{"name":"Shanghai Institute of Materia Medica, Chinese Academy of Sciences","place":["Shanghai, China"]},{"name":"University of Chinese Academy of Sciences","place":["Beijing, China"]},{"name":"School of Pharmaceutical Science and Technology","place":["Hangzhou, China"]},{"name":"Hangzhou Institute for Advanced Study, UCAS","place":["Hangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2025,11,12]]},"reference":[{"key":"ref1\/cit1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd3410"},{"key":"ref2\/cit2","doi-asserted-by":"publisher","DOI":"10.1039\/D3RA02133E"},{"key":"ref3\/cit3","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.3c01825"},{"key":"ref4\/cit4","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2023.103799"},{"key":"ref5\/cit5","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202411037"},{"key":"ref6\/cit6","doi-asserted-by":"publisher","DOI":"10.1039\/C7MD00571G"},{"key":"ref7\/cit7","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2017.09.023"},{"key":"ref8\/cit8","doi-asserted-by":"publisher","DOI":"10.1021\/jm3003203"},{"key":"ref9\/cit9","doi-asserted-by":"publisher","DOI":"10.1021\/jm800498e"},{"key":"ref10\/cit10","doi-asserted-by":"publisher","DOI":"10.1016\/S0149-2918(01)80109-0"},{"key":"ref11\/cit11","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2024.107059"},{"key":"ref12\/cit12","doi-asserted-by":"publisher","DOI":"10.1039\/D1MD00068C"},{"key":"ref13\/cit13","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2018.03.062"},{"key":"ref14\/cit14","doi-asserted-by":"publisher","DOI":"10.1021\/jm500973a"},{"key":"ref15\/cit15","doi-asserted-by":"publisher","DOI":"10.1002\/ardp.201600063"},{"key":"ref16\/cit16","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.9b01180"},{"key":"ref17\/cit17","doi-asserted-by":"publisher","DOI":"10.1038\/nrd1470"},{"key":"ref18\/cit18","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.104.001412"},{"key":"ref19\/cit19","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2020.103811"},{"key":"ref20\/cit20","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2017.5094"},{"key":"ref21\/cit21","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-016-0268-z"},{"key":"ref22\/cit22","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.112.197756"},{"key":"ref23\/cit23","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2017.05.027"},{"key":"ref24\/cit24","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-013-0886-7"},{"key":"ref25\/cit25","doi-asserted-by":"publisher","DOI":"10.1038\/s41571-018-0115-y"},{"key":"ref26\/cit26","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.9b00510"},{"key":"ref27\/cit27","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.2c01507"},{"key":"ref28\/cit28","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2020.09.002"},{"key":"ref29\/cit29","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.9b01828"},{"key":"ref30\/cit30","doi-asserted-by":"publisher","DOI":"10.1126\/science.abb4489"},{"key":"ref31\/cit31","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.4c03024"},{"key":"ref32\/cit32","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-024-01758-8"},{"key":"ref33\/cit33","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.3c00818"},{"key":"ref34\/cit34","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2023.115129"},{"key":"ref35\/cit35","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.17.9367"},{"key":"ref36\/cit36","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biophys.33.110502.140409"},{"key":"ref37\/cit37","doi-asserted-by":"publisher","DOI":"10.1039\/C5MD00518C"},{"key":"ref38\/cit38","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.01.006"},{"key":"ref39\/cit39","doi-asserted-by":"publisher","DOI":"10.1038\/nature12796"},{"key":"ref40\/cit40","doi-asserted-by":"publisher","DOI":"10.1126\/science.aad6204"},{"key":"ref41\/cit41","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-15-1105"},{"key":"ref42\/cit42","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1694-1"},{"key":"ref43\/cit43","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-19-1167"},{"key":"ref44\/cit44","doi-asserted-by":"publisher","DOI":"10.1007\/s40265-023-01839-y"},{"key":"ref45\/cit45","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.202000473"},{"key":"ref46\/cit46","doi-asserted-by":"publisher","DOI":"10.1038\/s41589-023-01537-6"},{"key":"ref47\/cit47","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1102376108"},{"key":"ref48\/cit48","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202008585"},{"key":"ref49\/cit49","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.1c03035"},{"key":"ref50\/cit50","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.9b02822"},{"key":"ref51\/cit51","doi-asserted-by":"publisher","DOI":"10.1039\/D1CS00231G"},{"key":"ref52\/cit52","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.26.14694"},{"key":"ref53\/cit53","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-5521(00)00014-4"},{"key":"ref54\/cit54","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.1c11053"},{"key":"ref55\/cit55","doi-asserted-by":"publisher","DOI":"10.1038\/nbt826"},{"key":"ref56\/cit56","doi-asserted-by":"publisher","DOI":"10.1038\/nature18002"},{"key":"ref57\/cit57","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2017.08.013"},{"key":"ref58\/cit58","doi-asserted-by":"publisher","DOI":"10.1002\/ijch.202200100"},{"key":"ref59\/cit59","doi-asserted-by":"publisher","DOI":"10.1038\/s41557-024-01601-1"},{"key":"ref60\/cit60","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200300721"},{"key":"ref61\/cit61","doi-asserted-by":"publisher","DOI":"10.1038\/s41589-024-01738-7"},{"key":"ref62\/cit62","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07318-y"},{"key":"ref63\/cit63","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.12.5381"},{"key":"ref64\/cit64","doi-asserted-by":"publisher","DOI":"10.1021\/acschembio.3c00803"},{"key":"ref65\/cit65","doi-asserted-by":"publisher","DOI":"10.1039\/C6MD00242K"},{"key":"ref66\/cit66","doi-asserted-by":"publisher","DOI":"10.1021\/acsptsci.1c00118"},{"key":"ref67\/cit67","doi-asserted-by":"publisher","DOI":"10.1002\/chem.201701644"},{"key":"ref68\/cit68","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.211"},{"key":"ref69\/cit69","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.7b04880"},{"key":"ref70\/cit70","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201412276"},{"key":"ref71\/cit71","doi-asserted-by":"publisher","DOI":"10.1021\/acsmedchemlett.2c00127"},{"key":"ref72\/cit72","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.3c08852"},{"key":"ref73\/cit73","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.5c01712"},{"key":"ref74\/cit74","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202505581"},{"key":"ref75\/cit75","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2019.09.012"},{"key":"ref76\/cit76","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.4c01416"},{"key":"ref77\/cit77","doi-asserted-by":"publisher","DOI":"10.1126\/science.4001944"},{"key":"ref78\/cit78","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-020-0733-7"},{"key":"ref79\/cit79","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.4c15713"},{"key":"ref80\/cit80","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.2c07375"},{"key":"ref81\/cit81","doi-asserted-by":"publisher","DOI":"10.1038\/s41589-025-01903-6"},{"key":"ref82\/cit82","doi-asserted-by":"publisher","DOI":"10.1021\/acssynbio.9b00419"},{"key":"ref83\/cit83","doi-asserted-by":"publisher","DOI":"10.1038\/35070613"},{"key":"ref84\/cit84","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0112028"},{"key":"ref85\/cit85","doi-asserted-by":"publisher","DOI":"10.1038\/nature06032"},{"key":"ref86\/cit86","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.23.12297"},{"key":"ref87\/cit87","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(97)01026-0"},{"key":"ref88\/cit88","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.4c15843"},{"key":"ref89\/cit89","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.3c04833"},{"key":"ref90\/cit90","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.4c07851"},{"key":"ref91\/cit91","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03819-2"},{"key":"ref92\/cit92","doi-asserted-by":"publisher","DOI":"10.1021\/ja00890a037"},{"key":"ref93\/cit93","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1971.tb31146.x"},{"key":"ref94\/cit94","doi-asserted-by":"publisher","DOI":"10.1021\/jm00308a011"},{"key":"ref95\/cit95","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201309399"},{"key":"ref96\/cit96","doi-asserted-by":"publisher","DOI":"10.1039\/C7OB02028G"},{"key":"ref97\/cit97","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.7b12788"},{"key":"ref98\/cit98","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.9b13652"},{"key":"ref99\/cit99","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2103513118"},{"key":"ref100\/cit100","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.5c00050"},{"key":"ref101\/cit101","doi-asserted-by":"publisher","DOI":"10.1038\/s41557-023-01304-z"},{"key":"ref102\/cit102","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.8b01087"},{"key":"ref103\/cit103","doi-asserted-by":"publisher","DOI":"10.1038\/s41557-024-01452-w"},{"key":"ref104\/cit104","doi-asserted-by":"publisher","DOI":"10.1021\/acs.orglett.1c01907"},{"key":"ref105\/cit105","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.4c01541"},{"key":"ref106\/cit106","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.8b00305"},{"key":"ref107\/cit107","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.5c03562"},{"key":"ref108\/cit108","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.2c00272"},{"key":"ref109\/cit109","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jcim.4c01591"},{"key":"ref110\/cit110","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.4c01616"},{"key":"ref111\/cit111","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-59870-4"},{"key":"ref112\/cit112","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.5c01663"},{"key":"ref113\/cit113","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.4c17827"},{"key":"ref114\/cit114","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202423524"},{"key":"ref115\/cit115","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biochem.8b00391"},{"key":"ref116\/cit116","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-17997-6"},{"key":"ref117\/cit117","doi-asserted-by":"publisher","DOI":"10.1021\/acschembio.8b01083"},{"key":"ref118\/cit118","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.9b13907"},{"key":"ref119\/cit119","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.4c05174"},{"key":"ref120\/cit120","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202424928"},{"key":"ref121\/cit121","doi-asserted-by":"publisher","DOI":"10.1021\/ar4001229"},{"key":"ref122\/cit122","doi-asserted-by":"publisher","DOI":"10.1038\/nsb1101-963"},{"key":"ref123\/cit123","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.0c00919"},{"key":"ref124\/cit124","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.1c02156"},{"key":"ref125\/cit125","doi-asserted-by":"publisher","DOI":"10.1021\/acscentsci.1c00616"}],"container-title":["JACS Au"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/jacsau.5c01134","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/jacsau.5c01134","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T11:07:30Z","timestamp":1768561650000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/doi\/10.1021\/jacsau.5c01134"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,12]]},"references-count":125,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2025,12,22]]}},"alternative-id":["10.1021\/jacsau.5c01134"],"URL":"https:\/\/doi.org\/10.1021\/jacsau.5c01134","relation":{},"ISSN":["2691-3704","2691-3704"],"issn-type":[{"value":"2691-3704","type":"print"},{"value":"2691-3704","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,12]]}}}