{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T04:28:44Z","timestamp":1789100924993,"version":"build-2803163510"},"reference-count":49,"publisher":"American Chemical Society (ACS)","issue":"9","license":[{"start":{"date-parts":[[2020,8,6]],"date-time":"2020-08-06T00:00:00Z","timestamp":1596672000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2020,8,6]],"date-time":"2020-08-06T00:00:00Z","timestamp":1596672000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2020,8,6]],"date-time":"2020-08-06T00:00:00Z","timestamp":1596672000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["INST 162\/500-1 FUGG"],"award-info":[{"award-number":["INST 162\/500-1 FUGG"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["Sp314\/13-1"],"award-info":[{"award-number":["Sp314\/13-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"name":"State of Hesse"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,9,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The obligate intracellular apicomplexan parasites Toxoplasma gondii and Besnoitia besnoiti are important causes of disease in both humans and cattle. To date, effective specific treatments are lacking for both infections. To counteract severe symptoms leading to, e.g., disabilities and even abortion in the case of human toxoplasmosis and bovine besnoitiosis, novel targets are required for development of drugs and vaccines. A promising emerging technique for molecular characterization of organisms is high-resolution atmospheric-pressure scanning microprobe matrix-assisted laser desorption\/ionization (AP-SMALDI) mass spectrometry imaging (MSI) which enables semiquantitative visualization of metabolite distributions. MSI was here used to trace and characterize lipid metabolites in primary bovine umbilical vein endothelial cells (BUVECs) upon infection with tachyzoites, an early and pathogenic fast-replicating life stage of T. gondii and B. besnoiti. A cell bulk, derived from noninfected controls and parasite-infected cell pellets, was analyzed by AP-SMALDI MSI in technical and biological triplicates. Multivariate statistical analysis including hierarchical clustering and principle component analysis revealed infection-specific metabolites in both positive- and negative-ion mode, identified by combining database search and LC-MS2 experiments. MSI analyses of host cell monolayers were conducted at 5 \u03bcm lateral resolution, allowing single apicomplexan-infected cells to be allocated. This is the first mass spectrometry imaging study on intracellular T. gondii and B. besnoiti infections and the first detailed metabolomic characterization of B. besnoiti tachyzoites. MSI was used here as an efficient tool to discriminate infected from noninfected cells at the single-cell level in vitro.<\/jats:p>","DOI":"10.1021\/jasms.0c00043","type":"journal-article","created":{"date-parts":[[2020,8,6]],"date-time":"2020-08-06T17:20:39Z","timestamp":1596734439000},"page":"1815-1824","source":"Crossref","is-referenced-by-count":17,"title":["Intracellular Parasites\n                    <i>Toxoplasma gondii<\/i>\n                    and\n                    <i>Besnoitia besnoiti<\/i>\n                    , Unveiled in Single Host\nCells Using AP-SMALDI MS Imaging"],"prefix":"10.1021","volume":"31","author":[{"given":"Patrik","family":"Kadesch","sequence":"first","affiliation":[{"name":"Justus Liebig University Giessen , , ,","place":["Giessen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Hollubarsch","sequence":"additional","affiliation":[{"name":"Justus Liebig University Giessen , , ,","place":["Giessen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1355-4048","authenticated-orcid":true,"given":"Stefanie","family":"Gerbig","sequence":"additional","affiliation":[{"name":"Justus Liebig University Giessen , , ,","place":["Giessen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars","family":"Schneider","sequence":"additional","affiliation":[{"name":"Justus Liebig University Giessen , , ,","place":["Giessen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liliana M. R.","family":"Silva","sequence":"additional","affiliation":[{"name":"Justus Liebig University Giessen , , ,","place":["Giessen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Hermosilla","sequence":"additional","affiliation":[{"name":"Justus Liebig University Giessen , , ,","place":["Giessen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anja","family":"Taubert","sequence":"additional","affiliation":[{"name":"Justus Liebig University Giessen , , ,","place":["Giessen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0179-5653","authenticated-orcid":true,"given":"Bernhard","family":"Spengler","sequence":"additional","affiliation":[{"name":"Justus Liebig University Giessen , , ,","place":["Giessen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2020,8,6]]},"reference":[{"key":"2026081505071644800_cit1","doi-asserted-by":"publisher","first-page":"22551","DOI":"10.1038\/srep22551","article-title":"Prevalence, incidence estimations, and risk factors\nof Toxoplasma gondii infection in Germany: a representative, cross-sectional,\nserological study","volume":"6","author":"Wilking","year":"2016","journal-title":"Sci. Rep."},{"key":"2026081505071644800_cit2","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1016\/j.vetpar.2010.08.013","article-title":"Bovine besnoitiosis: Epidemiological\nand clinical aspects","volume":"174","author":"Jacquiet","year":"2010","journal-title":"Vet. Parasitol."},{"key":"2026081505071644800_cit3","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1016\/j.vetpar.2011.01.027","article-title":"Exploring\nthe life cycle of Besnoitia besnoiti-Experimental infection of putative\ndefinitive and intermediate host species","volume":"178","author":"Basso","year":"2011","journal-title":"Vet.\nParasitol."},{"key":"2026081505071644800_cit4","doi-asserted-by":"publisher","first-page":"423","DOI":"10.1016\/j.ijpara.2003.12.009","article-title":"Toxoplasma\ngondii: the model apicomplexan","volume":"34","author":"Kim","year":"2004","journal-title":"Int. J. Parasitol."},{"key":"2026081505071644800_cit5","doi-asserted-by":"publisher","first-page":"1385","DOI":"10.1016\/j.ijpara.2009.04.003","article-title":"Toxoplasmosis\nsnapshots: Global status\nof Toxoplasma gondii seroprevalence and implications for pregnancy\nand congenital toxoplasmosis","volume":"39","author":"Pappas","year":"2009","journal-title":"Int. J. Parasitol."},{"key":"2026081505071644800_cit6","doi-asserted-by":"publisher","first-page":"1965","DOI":"10.1016\/S0140-6736(04)16412-X","article-title":"Toxoplasmosis","volume":"363","author":"Montoya","year":"2004","journal-title":"Lancet"},{"key":"2026081505071644800_cit7","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/S0020-7519(01)00308-3","article-title":"Characterisation\nof global protein expression by two-dimensional electrophoresis and\nmass spectrometry: proteomics of Toxoplasma gondii","volume":"32","author":"Cohen","year":"2002","journal-title":"Int. J. Parasitol."},{"key":"2026081505071644800_cit8","doi-asserted-by":"publisher","first-page":"R116","DOI":"10.1186\/gb-2008-9-7-r116","article-title":"The proteome of\nToxoplasma gondii: integration with the genome provides novel insights\ninto gene expression and annotation","volume":"9","author":"Xia","year":"2008","journal-title":"Genome\nBiol."},{"key":"2026081505071644800_cit9","doi-asserted-by":"publisher","first-page":"410","DOI":"10.1016\/j.chom.2011.09.004","article-title":"The Phosphoproteomes\nof Plasmodium falciparum and Toxoplasma gondii Reveal Unusual Adaptations\nWithin and Beyond the Parasites\u2019 Boundaries","volume":"10","author":"Treeck","year":"2011","journal-title":"Cell Host Microbe"},{"key":"2026081505071644800_cit10","doi-asserted-by":"publisher","first-page":"828","DOI":"10.1128\/IAI.01115-07","article-title":"Modulation of the host cell proteome\nby the intracellular\napicomplexan parasite Toxoplasma gondii","volume":"76","author":"Nelson","year":"2008","journal-title":"Infect.\nImmun."},{"key":"2026081505071644800_cit11","doi-asserted-by":"publisher","first-page":"13882","DOI":"10.1021\/bi7011993","article-title":"Lipidomic analysis of Toxoplasma gondii reveals unusual\npolar lipids","volume":"46","author":"Welti","year":"2007","journal-title":"Biochemistry"},{"key":"2026081505071644800_cit12","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1016\/j.molbiopara.2012.11.007","article-title":"Sphingolipid synthesis and scavenging\nin the intracellular apicomplexan parasite, Toxoplasma gondii","volume":"187","author":"Pratt","year":"2013","journal-title":"Mol. Biochem. Parasitol."},{"key":"2026081505071644800_cit13","doi-asserted-by":"publisher","first-page":"994","DOI":"10.1194\/jlr.M082891","article-title":"Toxoplasma gondii acetyl-CoA synthetase\nis involved in fatty acid elongation (of long fatty acid chains) during\ntachyzoite life stages","volume":"59","author":"Dubois","year":"2018","journal-title":"J. Lipid Res."},{"key":"2026081505071644800_cit14","doi-asserted-by":"publisher","first-page":"999","DOI":"10.1017\/S003118201300036X","article-title":"Identification\nof Besnoitia besnoiti proteins that showed differences in abundance\nbetween tachyzoite and bradyzoite stages by difference gel electrophoresis","volume":"140","author":"Fernandez-Garcia","year":"2013","journal-title":"Parasitology"},{"key":"2026081505071644800_cit15","doi-asserted-by":"publisher","first-page":"434","DOI":"10.1016\/j.vetpar.2014.09.003","article-title":"Proteomics reveals differences in\nprotein abundance\nand highly similar antigenic profiles between Besnoitia besnoiti and\nBesnoitia tarandi","volume":"205","author":"Garcia-Lunar","year":"2014","journal-title":"Vet. Parasitol."},{"key":"2026081505071644800_cit16","doi-asserted-by":"publisher","first-page":"2023","DOI":"10.1007\/s00436-016-4946-0","article-title":"Metabolic\nsignatures of Besnoitia besnoiti-infected\nendothelial host cells and blockage of key metabolic pathways indicate\nhigh glycolytic and glutaminolytic needs of the parasite","volume":"115","author":"Taubert","year":"2016","journal-title":"Parasitol. Res."},{"key":"2026081505071644800_cit17","doi-asserted-by":"publisher","first-page":"2314","DOI":"10.1194\/jlr.D016550","article-title":"A comprehensive\nmethod for lipid profiling by liquid chromatography-ion cyclotron\nresonance mass spectrometry","volume":"52","author":"Fauland","year":"2011","journal-title":"J. Lipid Res."},{"key":"2026081505071644800_cit18","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1016\/j.chroma.2012.03.074","article-title":"Comparison of sample preparation\napproaches for phospholipids profiling in human serum by liquid chromatography-tandem\nmass spectrometry","volume":"1240","author":"Ferreiro-Vera","year":"2012","journal-title":"Journal of Chromatography\nA"},{"key":"2026081505071644800_cit19","doi-asserted-by":"publisher","first-page":"703","DOI":"10.1016\/j.jasms.2004.01.007","article-title":"De novo sequencing,\npeptide composition analysis, and composition-based sequencing: a\nnew strategy employing accurate mass determination by fourier transform\nion cyclotron resonance mass spectrometry","volume":"15","author":"Spengler","year":"2004","journal-title":"J.\nAm. Soc. Mass Spectrom."},{"key":"2026081505071644800_cit20","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1255\/ejms.840","article-title":"Accurate mass\nas a bioinformatic parameter in data-to-knowledge conversion: Fourier\ntransform ion cyclotron resonance mass spectrometry for peptide de\nnovo sequencing","volume":"13","author":"Spengler","year":"2007","journal-title":"Eur. J. Mass Spectrom."},{"key":"2026081505071644800_cit21","doi-asserted-by":"publisher","first-page":"1808","DOI":"10.1016\/j.jasms.2008.08.005","article-title":"Mass-Based Classification\n(MBC) of Peptides: Highly\nAccurate Precursor Ion Mass Values Can Be Used to Directly Recognize\nPeptide Phosphorylation","volume":"19","author":"Spengler","year":"2008","journal-title":"J. Am. Soc. Mass Spectrom."},{"key":"2026081505071644800_cit22","doi-asserted-by":"publisher","first-page":"367","DOI":"10.1002\/mas.20023","article-title":"Shotgun lipidomics:\nElectrospray ionization mass spectrometric\nanalysis and quantitation of cellular lipidomes directly from crude\nextracts of biological samples","volume":"24","author":"Han","year":"2005","journal-title":"Mass Spectrom.\nRev."},{"key":"2026081505071644800_cit23","doi-asserted-by":"publisher","first-page":"S9","DOI":"10.1194\/jlr.R800095-JLR200","article-title":"Update\nof the LIPID MAPS comprehensive\nclassification system for lipids","volume":"50","author":"Fahy","year":"2009","journal-title":"J. Lipid Res."},{"key":"2026081505071644800_cit24","doi-asserted-by":"publisher","first-page":"4273","DOI":"10.1039\/C8AN00659H","article-title":"Strategy for marker-based differentiation\nof pro- and anti-inflammatory macrophages using matrix-assisted laser\ndesorption\/ionization mass spectrometry imaging","volume":"143","author":"Khamehgir-Silz","year":"2018","journal-title":"Analyst"},{"key":"2026081505071644800_cit25","doi-asserted-by":"publisher","first-page":"2766","DOI":"10.1111\/bph.13555","article-title":"Epithelial propionyl-\nand butyrylcholine as novel regulators of colonic\nion transport","volume":"173","author":"Moreno","year":"2016","journal-title":"Br. J. Pharmacol."},{"key":"2026081505071644800_cit26","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1007\/s00216-011-4990-7","volume":"401","author":"Ro\u0308mpp","year":"2011","journal-title":"Anal. Bioanal. Chem."},{"key":"2026081505071644800_cit27","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1586\/14789450.3.1.87","article-title":"Proteomics of the human malaria parasite\nPlasmodium\nfalciparum","volume":"3","author":"Sims","year":"2006","journal-title":"Expert Rev. Proteomics"},{"key":"2026081505071644800_cit28","doi-asserted-by":"publisher","first-page":"11309","DOI":"10.1021\/acs.analchem.5b02781","article-title":"Phospholipid\nTopography of Whole-Body Sections of the Anopheles stephensi Mosquito,\nCharacterized by High-Resolution Atmospheric- Pressure Scanning Microprobe\nMatrix-Assisted Laser Desorption\/Ionization Mass Spectrometry Imaging","volume":"87","author":"Khalil","year":"2015","journal-title":"Anal. Chem."},{"key":"2026081505071644800_cit29","unstructured":"Spengler, B.; Hubert, M.; Kaufmann, R.\n          MALDI Ion Imaging and Biological Ion Imaging with\na new Scanning UV-Laser Microprobe. Proceedings\nof the 42nd Annual Conference on Mass Spectrometry and Allied Topics, Chicago, Illinois, May 29\u2013June 3, 1994, p. 1041."},{"key":"2026081505071644800_cit30","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1038\/nmeth.4071","article-title":"Atmospheric\npressure MALDI mass spectrometry\nimaging of tissues and cells at 1.4-\u03bcm lateral resolution","volume":"14","author":"Kompauer","year":"2017","journal-title":"Nat. Methods"},{"key":"2026081505071644800_cit31","doi-asserted-by":"publisher","first-page":"1137","DOI":"10.1194\/jlr.D700041-JLR200","article-title":"Lipid extraction by methyl-tert-butyl ether for high-throughput\nlipidomics","volume":"49","author":"Matyash","year":"2008","journal-title":"J. Lipid Res."},{"key":"2026081505071644800_cit32","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1016\/j.jchromb.2014.01.011","article-title":"A versatile\nultra-high performance LC-MS method for lipid profiling","volume":"951\u2013952","author":"Knittelfelder","year":"2014","journal-title":"J. Chromatogr. B: Anal. Technol. Biomed. Life Sci."},{"key":"2026081505071644800_cit33","doi-asserted-by":"publisher","first-page":"331","DOI":"10.1186\/s12859-017-1744-3","article-title":"LipidMatch:\nan automated workflow for rule-based lipid identification using untargeted\nhigh-resolution tandem mass spectrometry data","volume":"18","author":"Koelmel","year":"2017","journal-title":"BMC Bioinf."},{"key":"2026081505071644800_cit34","doi-asserted-by":"publisher","first-page":"572","DOI":"10.1093\/bioinformatics\/btq699","article-title":"Lipid Data Analyzer: unattended identification\nand quantitation of lipids in LC-MS data","volume":"27","author":"Hartler","year":"2011","journal-title":"Bioinformatics"},{"key":"2026081505071644800_cit35","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1007\/978-1-60761-987-1_12","article-title":"imzML: Imaging Mass\nSpectrometry Markup Language: A common data format for mass spectrometry\nimaging","volume":"696","author":"Rompp","year":"2011","journal-title":"Methods Mol. Biol. (N. Y., NY, U. S.)"},{"key":"2026081505071644800_cit36","doi-asserted-by":"publisher","first-page":"918","DOI":"10.1038\/nbt.2377","article-title":"A cross-platform\ntoolkit\nfor mass spectrometry and proteomics","volume":"30","author":"Chambers","year":"2012","journal-title":"Nat. Biotechnol."},{"key":"2026081505071644800_cit37","doi-asserted-by":"publisher","first-page":"5111","DOI":"10.1016\/j.jprot.2012.05.035","article-title":"Inclusive\nsharing of mass spectrometry\nimaging data requires a converter for all","volume":"75","author":"Race","year":"2012","journal-title":"J.\nProteomics"},{"key":"2026081505071644800_cit38","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1038\/nmeth.4072","article-title":"FDR-controlled\nmetabolite annotation for high-resolution imaging mass spectrometry","volume":"14","author":"Palmer","year":"2017","journal-title":"Nat. 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Methods"},{"key":"2026081505071644800_cit41","doi-asserted-by":"publisher","first-page":"S12","DOI":"10.1186\/1471-2105-13-S16-S12","article-title":"1D and 2D annotation enrichment: a statistical method integrating\nquantitative proteomics with complementary high-throughput data","volume":"13","author":"Cox","year":"2012","journal-title":"BMC Bioinf."},{"key":"2026081505071644800_cit42","doi-asserted-by":"publisher","first-page":"718","DOI":"10.1007\/s13361-013-0607-z","article-title":"MSiReader: An Open-Source\nInterface to View and Analyze High Resolving Power MS Imaging Files\non Matlab Platform","volume":"24","author":"Robichaud","year":"2013","journal-title":"J. Am. Soc. Mass Spectrom."},{"key":"2026081505071644800_cit43","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1007\/s13361-017-1809-6","article-title":"MSiReader v1.0:\nEvolving Open-Source Mass Spectrometry Imaging Software for Targeted\nand Untargeted Analyses","volume":"29","author":"Bokhart","year":"2018","journal-title":"J. Am. Soc. Mass Spectrom."},{"key":"2026081505071644800_cit44","doi-asserted-by":"publisher","first-page":"1243259","DOI":"10.1126\/science.1243259","article-title":"Single-Cell\nMetabolomics: Analytical and Biological Perspectives","volume":"342","author":"Zenobi","year":"2013","journal-title":"Science"},{"key":"2026081505071644800_cit45","doi-asserted-by":"publisher","first-page":"e0199239","DOI":"10.1371\/journal.pone.0199239","article-title":"Optimizing colormaps with consideration\nfor color vision deficiency to enable accurate interpretation of scientific\ndata","volume":"13","author":"Nun\u0303ez","year":"2018","journal-title":"PLoS One"},{"key":"2026081505071644800_cit46","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1016\/S0166-6851(02)00281-5","article-title":"Phospholipids\nin parasitic protozoa","volume":"126","author":"Vial","year":"2003","journal-title":"Mol. Biochem. Parasitol."},{"key":"2026081505071644800_cit47","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1083\/jcb.149.1.167","article-title":"Toxoplasma\ngondii exploits host low-density\nlipoprotein receptor-mediated endocytosis for cholesterol acquisition","volume":"149","author":"Coppens","year":"2000","journal-title":"J. Cell Biol."},{"key":"2026081505071644800_cit48","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1186\/1476-511X-4-10","article-title":"Role of cholesterol in parasitic\ninfections","volume":"4","author":"Bansal","year":"2005","journal-title":"Lipids Health Dis."},{"key":"2026081505071644800_cit49","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1042\/BJ20080795","article-title":"Lipidomic\nanalysis of Toxoplasma gondii tachyzoites rhoptries: further insights\ninto the role of cholesterol","volume":"415","author":"Besteiro","year":"2008","journal-title":"Biochem. J."}],"container-title":["Journal of the American Society for Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/jasms.0c00043","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/jamsef\/article-pdf\/31\/9\/1815\/14267297\/js0c00043.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/jamsef\/article-pdf\/31\/9\/1815\/14267297\/js0c00043.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T13:38:09Z","timestamp":1786801089000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/jamsef\/article\/31\/9\/1815\/1372931\/Intracellular-Parasites-Toxoplasma-gondii-and"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,6]]},"references-count":49,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2020,8,6]]},"published-print":{"date-parts":[[2020,9,2]]}},"URL":"https:\/\/doi.org\/10.1021\/jasms.0c00043","relation":{},"ISSN":["1044-0305","1879-1123"],"issn-type":[{"value":"1044-0305","type":"print"},{"value":"1879-1123","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8,6]]}}}