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While advances in neuroimaging have improved diagnostic capabilities, the complementary use of molecular biomarkers can provide clinicians with additional insight into the nature and severity of TBI. In this study, a panel of eight metabolites involved in distinct pathophysiological processes related to concussion was quantified using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS\/MS). Specifically, the newly developed method can simultaneously determine urinary concentrations of glutamic acid, homovanillic acid, 5-hydroxyindoleacetic acid, methionine sulfoxide, lactic acid, pyruvic acid, N-acetylaspartic acid, and F2\u03b1-isoprostane without intensive sample preparation or preconcentration. The method was systematically validated to assess sensitivity (method detection limits: 1\u201320 \u03bcg\/L), accuracy (81\u2013124% spike recoveries in urine), and reproducibility (relative standard deviation: 4\u201312%). The method was ultimately applied to a small cohort of urine specimens obtained from healthy college student volunteers. The method presented here provides a new technique to facilitate future work aiming to assess the clinical efficacy of these putative biomarkers for noninvasive assessment of TBI.<\/jats:p>","DOI":"10.1021\/jasms.0c00181","type":"journal-article","created":{"date-parts":[[2020,7,23]],"date-time":"2020-07-23T10:38:36Z","timestamp":1595500716000},"page":"1910-1917","source":"Crossref","is-referenced-by-count":10,"title":["Simultaneous Determination of Eight Urinary Metabolites\nby HPLC-MS\/MS for Noninvasive Assessment of Traumatic Brain Injury"],"prefix":"10.1021","volume":"31","author":[{"given":"Austin","family":"Sigler","sequence":"first","affiliation":[{"name":"Missouri University of Science and Technology , , , ,","place":["Rolla, Missouri, United States, 65409"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolong","family":"He","sequence":"additional","affiliation":[{"name":"Missouri University of Science and Technology , , , ,","place":["Rolla, Missouri, United States, 65409"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mousumi","family":"Bose","sequence":"additional","affiliation":[{"name":"Missouri University of Science and Technology , , , ,","place":["Rolla, Missouri, United States, 65409"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre","family":"Cristea","sequence":"additional","affiliation":[{"name":"Missouri University of Science and Technology , , , ,","place":["Rolla, Missouri, United States, 65409"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenyan","family":"Liu","sequence":"additional","affiliation":[{"name":"Missouri University of Science and Technology , , , ,","place":["Rolla, Missouri, United States, 65409"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6064-3257","authenticated-orcid":true,"given":"Paul Ki-souk","family":"Nam","sequence":"additional","affiliation":[{"name":"Missouri University of Science and Technology , , , ,","place":["Rolla, Missouri, United States, 65409"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donald","family":"James","sequence":"additional","affiliation":[{"name":"Phelps Health , , ,","place":["Rolla, Missouri, United States, 65401"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0729-6743","authenticated-orcid":true,"given":"Casey","family":"Burton","sequence":"additional","affiliation":[{"name":"Missouri University of Science and Technology , , , ,","place":["Rolla, Missouri, United States, 65409"]},{"name":"Phelps Health , , ,","place":["Rolla, Missouri, United States, 65401"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0801-5683","authenticated-orcid":true,"given":"Honglan","family":"Shi","sequence":"additional","affiliation":[{"name":"Missouri University of Science and Technology , , , ,","place":["Rolla, Missouri, United States, 65409"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2020,7,23]]},"reference":[{"key":"2026081505073382400_cit1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s11916-015-0506-z","article-title":"Update on the epidemiology\nof concussion\/mild traumatic brain injury","volume":"19","author":"Voss","year":"2015","journal-title":"Curr.\nPain Headache Rep."},{"key":"2026081505073382400_cit2","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1080\/13854040701760981","article-title":"Official Position\nof the Military TBI Task Force on the Role of Neuropsychology and\nRehabilitation Psychology in the Evaluation, Management, and Research\nof Military Veterans with Traumatic Brain Injury","volume":"22","author":"McCrea","year":"2008","journal-title":"Clin. 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