{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T23:37:46Z","timestamp":1790293066694,"version":"4.1.0"},"reference-count":61,"publisher":"American Chemical Society (ACS)","issue":"6","license":[{"start":{"date-parts":[[2023,5,1]],"date-time":"2023-05-01T00:00:00Z","timestamp":1682899200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2023,5,1]],"date-time":"2023-05-01T00:00:00Z","timestamp":1682899200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2023,5,1]],"date-time":"2023-05-01T00:00:00Z","timestamp":1682899200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/100000165","name":"Division of Chemistry","doi-asserted-by":"publisher","award":["CHE-1903967"],"award-info":[{"award-number":["CHE-1903967"]}],"id":[{"id":"10.13039\/100000165","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Am. Soc. Mass Spectrom."],"published-print":{"date-parts":[[2023,6,7]]},"DOI":"10.1021\/jasms.3c00038","type":"journal-article","created":{"date-parts":[[2023,5,2]],"date-time":"2023-05-02T02:53:40Z","timestamp":1682996020000},"page":"1086-1095","source":"Crossref","is-referenced-by-count":5,"title":["Analysis of Protein Glycosylation after Rapid Digestion Using Protease-Containing Membranes in Spin Columns"],"prefix":"10.1021","volume":"34","author":[{"given":"Weikai","family":"Cao","sequence":"first","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, Indiana 46556, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4553-5143","authenticated-orcid":true,"given":"Merlin L.","family":"Bruening","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, Indiana 46556, United States"},{"name":"Department of Chemistry, University of Notre Dame, Notre Dame, Indiana 46556, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2023,5,1]]},"reference":[{"key":"ref1\/cit1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3383"},{"key":"ref2\/cit2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3982"},{"key":"ref3\/cit3","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pathol-012414-040438"},{"key":"ref4\/cit4","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwq147"},{"key":"ref5\/cit5","doi-asserted-by":"publisher","DOI":"10.1021\/pr301112x"},{"key":"ref6\/cit6","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwr027"},{"key":"ref7\/cit7","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwl053"},{"key":"ref8\/cit8","doi-asserted-by":"publisher","DOI":"10.4161\/mabs.26453"},{"key":"ref9\/cit9","doi-asserted-by":"publisher","DOI":"10.1021\/acschembio.5b01035"},{"key":"ref10\/cit10","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2015.02.004"},{"key":"ref11\/cit11","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V73.1.90.90"},{"key":"ref12\/cit12","doi-asserted-by":"publisher","DOI":"10.1515\/bchm3.1986.367.1.433"},{"key":"ref13\/cit13","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.12.028"},{"key":"ref14\/cit14","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.02.058"},{"key":"ref15\/cit15","doi-asserted-by":"publisher","DOI":"10.1007\/s10719-015-9626-2"},{"key":"ref16\/cit16","unstructured":"Genovis\u202f\u226b OpeRATOR. https:\/\/www.genovis.com\/smartenzymes\/glycan-profiling\/operator\/ (accessed 2023-04-29)."},{"key":"ref17\/cit17","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-016-9996-8"},{"key":"ref18\/cit18","doi-asserted-by":"publisher","DOI":"10.1021\/ac702343a"},{"key":"ref19\/cit19","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5b03496"},{"key":"ref20\/cit20","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.7b02169"},{"key":"ref21\/cit21","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2021.676100"},{"key":"ref22\/cit22","doi-asserted-by":"publisher","DOI":"10.1021\/pr700586j"},{"key":"ref23\/cit23","doi-asserted-by":"publisher","DOI":"10.1007\/s13361-014-1015-8"},{"key":"ref24\/cit24","doi-asserted-by":"publisher","DOI":"10.1021\/ac101857j"},{"key":"ref25\/cit25","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5b02739"},{"key":"ref26\/cit26","doi-asserted-by":"publisher","DOI":"10.1039\/C8AN00969D"},{"key":"ref27\/cit27","doi-asserted-by":"publisher","DOI":"10.1021\/ac990731l"},{"key":"ref28\/cit28","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5b03679"},{"key":"ref29\/cit29","unstructured":"Trypsin Spin\nColumns for Proteomics Immobilized Trypsin. https:\/\/www.sigmaaldrich.com\/US\/en\/product\/sigma\/tt0010 (accessed 2022-11-24)."},{"key":"ref30\/cit30","unstructured":"Immobilized Trypsin. https:\/\/www.promega.com\/products\/mass-spectrometry\/trypsin\/immobilized-trypsin\/ (accessed 2022-11-24)."},{"key":"ref31\/cit31","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.6b01956"},{"key":"ref32\/cit32","unstructured":"Empty Spin Columns. Bio-Rad Laboratories. https:\/\/www.bio-rad.com\/en-us\/product\/empty-spin-columns?ID=612dbf40-5acd-468f-8eef-b855ce941865 (accessed 2022-11-25)."},{"key":"ref33\/cit33","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.0c01794"},{"key":"ref34\/cit34","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-29530-y"},{"key":"ref35\/cit35","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-021-01306-0"},{"key":"ref36\/cit36","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-021-03621-z"},{"key":"ref37\/cit37","doi-asserted-by":"publisher","DOI":"10.1007\/s13361-013-0823-6"},{"key":"ref38\/cit38","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.0c00051"},{"key":"ref39\/cit39","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.9b00594"},{"key":"ref40\/cit40","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.9b03808"},{"key":"ref41\/cit41","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2014.06.019"},{"key":"ref42\/cit42","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.0c00050"},{"key":"ref43\/cit43","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.0c05149"},{"key":"ref44\/cit44","doi-asserted-by":"publisher","DOI":"10.1021\/ac101856r"},{"key":"ref45\/cit45","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.7b02732"},{"key":"ref46\/cit46","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2022.339828"},{"key":"ref47\/cit47","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2020.02.042"},{"key":"ref48\/cit48","doi-asserted-by":"publisher","DOI":"10.1016\/1044-0305(95)00532-3"},{"key":"ref49\/cit49","doi-asserted-by":"publisher","DOI":"10.1002\/jms.4348"},{"key":"ref50\/cit50","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.9b03750"},{"key":"ref51\/cit51","doi-asserted-by":"publisher","DOI":"10.1080\/19420862.2015.1045173"},{"key":"ref52\/cit52","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2015.04.012"},{"key":"ref53\/cit53","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2012.062"},{"key":"ref54\/cit54","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-642-48380-6","volume-title":"Specificity of Proteolysis","author":"Keil B.","year":"1992"},{"key":"ref55\/cit55","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2020.08.004"},{"key":"ref56\/cit56","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.RA120.002295"},{"key":"ref57\/cit57","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.0c03173"},{"key":"ref58\/cit58","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M112.023259"},{"key":"ref59\/cit59","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-021-03433-1"},{"key":"ref60\/cit60","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymgme.2011.12.012"},{"key":"ref61\/cit61","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.9b01698"}],"container-title":["Journal of the American Society for Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/jasms.3c00038","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/jasms.3c00038","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,7]],"date-time":"2023-06-07T08:47:30Z","timestamp":1686127650000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/doi\/10.1021\/jasms.3c00038"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,1]]},"references-count":61,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2023,6,7]]}},"alternative-id":["10.1021\/jasms.3c00038"],"URL":"https:\/\/doi.org\/10.1021\/jasms.3c00038","relation":{},"ISSN":["1044-0305","1879-1123"],"issn-type":[{"value":"1044-0305","type":"print"},{"value":"1879-1123","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,1]]}}}