{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T04:42:24Z","timestamp":1792471344765,"version":"4.5.0"},"reference-count":61,"publisher":"American Chemical Society (ACS)","issue":"5","license":[{"start":{"date-parts":[[2024,3,27]],"date-time":"2024-03-27T00:00:00Z","timestamp":1711497600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100019109","name":"Environmental Molecular Sciences Laboratory","doi-asserted-by":"publisher","award":["60268"],"award-info":[{"award-number":["60268"]}],"id":[{"id":"10.13039\/100019109","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["75N93022C00035"],"award-info":[{"award-number":["75N93022C00035"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006132","name":"Office of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006132","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Structure-based drug design, which relies on precise understanding of the target protein and its interaction with the drug candidate, is dramatically expedited by advances in computational methods for candidate prediction. Yet, the accuracy needs to be improved with more structural data from high throughput experiments, which are challenging to generate, especially for dynamic and weak associations. Herein, we applied native mass spectrometry (native MS) to rapidly characterize ligand binding of an allosteric heterodimeric complex of SARS-CoV-2 nonstructural proteins (nsp) nsp10 and nsp16 (nsp10\/16), a complex essential for virus survival in the host and thus a desirable drug target. Native MS showed that the dimer is in equilibrium with monomeric states in solution. Consistent with the literature, well characterized small cosubstrate, RNA substrate, and product bind with high specificity and affinity to the dimer but not the free monomers. Unsuccessfully designed ligands bind indiscriminately to all forms. Using neutral gas collision, the nsp16 monomer with bound cosubstrate can be released from the holo dimer complex, confirming the binding to nsp16 as revealed by the crystal structure. However, we observed an unusual migration of the endogenous zinc ions bound to nsp10 to nsp16 after collisional dissociation. The metal migration can be suppressed by using surface collision with reduced precursor charge states, which presumably resulted in minimal gas-phase structural rearrangement and highlighted the importance of complementary techniques. With minimal sample input (\u223c\u03bcg), native MS can rapidly detect ligand binding affinities and locations in dynamic multisubunit protein complexes, demonstrating the potential of an \u201call-in-one\u201d native MS assay for rapid structural profiling of protein-to-AI-based compound systems to expedite drug discovery.<\/jats:p>","DOI":"10.1021\/jasms.3c00453","type":"journal-article","created":{"date-parts":[[2024,3,27]],"date-time":"2024-03-27T10:07:25Z","timestamp":1711534045000},"page":"912-921","source":"Crossref","is-referenced-by-count":8,"title":["Native Mass Spectrometry\nDissects the Structural Dynamics\nof an Allosteric Heterodimer of SARS-CoV\u20112 Nonstructural Proteins"],"prefix":"10.1021","volume":"35","author":[{"given":"Stephanie\nM.","family":"Thibert","sequence":"first","affiliation":[{"name":"Pacific Northwest National Laboratory , , , ,","place":["Richland, Washington, United States, 99354"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deseree J.","family":"Reid","sequence":"additional","affiliation":[{"name":"Pacific Northwest National Laboratory , , , ,","place":["Richland, Washington, United States, 99354"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2304-2540","authenticated-orcid":true,"given":"Jesse W.","family":"Wilson","sequence":"additional","affiliation":[{"name":"Pacific Northwest National Laboratory , , , ,","place":["Richland, Washington, United States, 99354"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rohith","family":"Varikoti","sequence":"additional","affiliation":[{"name":"Pacific Northwest National Laboratory , , , ,","place":["Richland, Washington, United States, 99354"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Maltseva","sequence":"additional","affiliation":[{"name":"University of Chicago , , , ,","place":["Chicago, Illinois, United States, 60637"]},{"name":"Argonne National Laboratory , , , ,","place":["Argonne, Illinois, United States, 60439"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine J.","family":"Schultz","sequence":"additional","affiliation":[{"name":"Pacific Northwest National Laboratory , , , ,","place":["Richland, Washington, United States, 99354"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5571-7418","authenticated-orcid":true,"given":"Agustin","family":"Kruel","sequence":"additional","affiliation":[{"name":"Pacific Northwest National Laboratory , , , ,","place":["Richland, Washington, United States, 99354"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9838-1803","authenticated-orcid":true,"given":"Gyorgy","family":"Babnigg","sequence":"additional","affiliation":[{"name":"University of Chicago , , , ,","place":["Chicago, Illinois, United States, 60637"]},{"name":"Argonne National Laboratory , , , ,","place":["Argonne, Illinois, United States, 60439"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrzej","family":"Joachimiak","sequence":"additional","affiliation":[{"name":"University of Chicago , , , ,","place":["Chicago, Illinois, United States, 60637"]},{"name":"Argonne National Laboratory , , , ,","place":["Argonne, Illinois, United States, 60439"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6713-2129","authenticated-orcid":true,"given":"Neeraj","family":"Kumar","sequence":"additional","affiliation":[{"name":"Pacific Northwest National Laboratory , , , ,","place":["Richland, Washington, United States, 99354"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3575-3224","authenticated-orcid":true,"given":"Mowei","family":"Zhou","sequence":"additional","affiliation":[{"name":"Pacific Northwest National Laboratory , , , ,","place":["Richland, Washington, United States, 99354"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2024,3,27]]},"reference":[{"issue":"11","key":"2026081707105279000_cit1","doi-asserted-by":"publisher","first-page":"2783","DOI":"10.3390\/ijms20112783","article-title":"A Structure-Based Drug Discovery\nParadigm","volume":"20","author":"Batool","year":"2019","journal-title":"International Journal of Molecular\nSciences"},{"issue":"18","key":"2026081707105279000_cit2","doi-asserted-by":"publisher","first-page":"10520","DOI":"10.1021\/acs.chemrev.8b00728","article-title":"Concepts of Artificial\nIntelligence for Computer-Assisted Drug Discovery","volume":"119","author":"Yang","year":"2019","journal-title":"Chem. Rev."},{"key":"2026081707105279000_cit3","doi-asserted-by":"publisher","first-page":"6761","DOI":"10.3390\/molecules26226761","article-title":"Artificial Intelligence\nfor Autonomous Molecular Design: A Perspective","volume":"26","author":"Joshi","year":"2021","journal-title":"Molecules"},{"issue":"5","key":"2026081707105279000_cit4","doi-asserted-by":"publisher","first-page":"1438","DOI":"10.1021\/acs.jcim.2c01377","article-title":"AI-Accelerated\nDesign of Targeted Covalent Inhibitors\nfor SARS-CoV-2","volume":"63","author":"Joshi","year":"2023","journal-title":"J. Chem. Inf. Model."},{"issue":"8","key":"2026081707105279000_cit5","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1007\/s10822-023-00509-1","article-title":"Integrated data-driven and experimental\napproaches to accelerate lead optimization targeting SARS-CoV-2 main\nprotease","volume":"37","author":"Varikoti","year":"2023","journal-title":"Journal of Computer-Aided Molecular\nDesign"},{"issue":"48","key":"2026081707105279000_cit6","doi-asserted-by":"publisher","first-page":"12918","DOI":"10.1039\/D0SC04392C","article-title":"Higher-order structural\ncharacterisation\nof native proteins and complexes by top-down mass spectrometry","volume":"11","author":"Zhou","year":"2020","journal-title":"Chem. Sci."},{"key":"2026081707105279000_cit7","doi-asserted-by":"publisher","first-page":"837901","DOI":"10.3389\/fmolb.2022.837901","article-title":"High-Throughput\nNative Mass Spectrometry Screening in Drug Discovery","volume":"9","author":"Gavriilidou","year":"2022","journal-title":"Front Mol. Biosci"},{"key":"2026081707105279000_cit8","doi-asserted-by":"publisher","DOI":"10.3389\/frans.2022.1014017","article-title":"Metabolite-protein interactions: Native mass spectrometry\nand collision induced affinity selection mass spectrometry in natural\nproduct screening","author":"Gu","year":"2022","journal-title":"Frontiers in Analytical Science"},{"issue":"8","key":"2026081707105279000_cit9","doi-asserted-by":"publisher","first-page":"7267","DOI":"10.1021\/acs.chemrev.2c00085","article-title":"Introduction: Mass\nSpectrometry Applications in Structural\nBiology","volume":"122","author":"Pukala","year":"2022","journal-title":"Chem. Rev."},{"issue":"3","key":"2026081707105279000_cit10","doi-asserted-by":"publisher","first-page":"1132","DOI":"10.1038\/s41596-019-0281-0","article-title":"Rapid online\nbuffer exchange for screening of proteins, protein complexes and cell\nlysates by native mass spectrometry","volume":"15","author":"VanAernum","year":"2020","journal-title":"Nat. Protoc."},{"issue":"7","key":"2026081707105279000_cit11","doi-asserted-by":"publisher","first-page":"1528","DOI":"10.1021\/jasms.3c00041","article-title":"A Simple and\nFlexible Infusion Platform for Automated Native Mass Spectrometry\nAnalysis","volume":"34","author":"Thibert","year":"2023","journal-title":"J. Am. Soc. Mass Spectrom."},{"key":"2026081707105279000_cit12","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2022.837901","article-title":"High-Throughput\nNative Mass Spectrometry Screening in Drug Discovery","author":"Gavriilidou","year":"2022","journal-title":"Frontiers in Molecular Biosciences"},{"issue":"2","key":"2026081707105279000_cit13","doi-asserted-by":"publisher","first-page":"2186","DOI":"10.1021\/acs.analchem.9b04826","article-title":"Novel Interface for High-Throughput\nAnalysis of Biotherapeutics\nby Electrospray Mass Spectrometry","volume":"92","author":"Park","year":"2020","journal-title":"Anal. Chem."},{"issue":"8","key":"2026081707105279000_cit14","doi-asserted-by":"publisher","first-page":"7269","DOI":"10.1021\/acs.chemrev.1c00212","article-title":"High-Resolution Native Mass Spectrometry","volume":"122","author":"Tamara","year":"2022","journal-title":"Chem. Rev."},{"key":"2026081707105279000_cit15","doi-asserted-by":"publisher","first-page":"7327","DOI":"10.1021\/acs.chemrev.1c00293","article-title":"Protein-Small\nMolecule Interactions\nin Native Mass Spectrometry","volume":"122","author":"Bennett","year":"2022","journal-title":"Chem. Rev."},{"issue":"48","key":"2026081707105279000_cit16","doi-asserted-by":"publisher","first-page":"12918","DOI":"10.1039\/D0SC04392C","article-title":"Higher-order\nstructural characterisation\nof native proteins and complexes by top-down mass spectrometry","volume":"11","author":"Zhou","year":"2020","journal-title":"Chemical Science"},{"key":"2026081707105279000_cit17","doi-asserted-by":"publisher","first-page":"637","DOI":"10.1016\/S1387-3806(01)00428-6","article-title":"Gas-phase dissociation\nof hemoglobin","volume":"210\u2013211","author":"Versluis","year":"2001","journal-title":"Int. J. Mass Spectrom."},{"issue":"6","key":"2026081707105279000_cit18","doi-asserted-by":"publisher","first-page":"1402","DOI":"10.1021\/ac0110552","article-title":"A tandem\nmass spectrometer for improved transmission and analysis of large\nmacromolecular assemblies","volume":"74","author":"Sobott","year":"2002","journal-title":"Anal. Chem."},{"issue":"10","key":"2026081707105279000_cit19","doi-asserted-by":"publisher","first-page":"1573","DOI":"10.1007\/s13361-013-0712-z","article-title":"Dissociation of\nMultisubunit Protein-Ligand Complexes in the Gas Phase. Evidence for\nLigand Migration","volume":"24","author":"Zhang","year":"2013","journal-title":"Journal of The American Society\nfor Mass Spectrometry"},{"issue":"4","key":"2026081707105279000_cit20","doi-asserted-by":"publisher","first-page":"723","DOI":"10.1007\/s13361-017-1882-x","article-title":"Surface Induced\nDissociation Coupled with High Resolution Mass Spectrometry Unveils\nHeterogeneity of a 211 kDa Multicopper Oxidase Protein Complex","volume":"29","author":"Zhou","year":"2018","journal-title":"J. Am. Soc. Mass Spectrom."},{"issue":"21","key":"2026081707105279000_cit21","doi-asserted-by":"publisher","first-page":"12796","DOI":"10.1021\/acs.analchem.8b03263","article-title":"Localization of\nProtein Complex Bound Ligands by Surface-Induced Dissociation High-Resolution\nMass Spectrometry","volume":"90","author":"Busch","year":"2018","journal-title":"Anal. Chem."},{"issue":"5","key":"2026081707105279000_cit22","doi-asserted-by":"publisher","first-page":"3611","DOI":"10.1021\/acs.analchem.8b05605","article-title":"Surface-Induced Dissociation of Noncovalent Protein\nComplexes in an Extended Mass Range Orbitrap Mass Spectrometer","volume":"91","author":"VanAernum","year":"2019","journal-title":"Anal. Chem."},{"issue":"19","key":"2026081707105279000_cit23","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2102516118","article-title":"Structural\nbasis for\nbacktracking by the SARS-CoV-2 replication-transcription complex","volume":"118","author":"Malone","year":"2021","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"21","key":"2026081707105279000_cit24","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2100170118","article-title":"2\u2032-O methylation\nof RNA cap in SARS-CoV-2\ncaptured by serial crystallography","volume":"118","author":"Wilamowski","year":"2021","journal-title":"Proc. Natl.\nAcad. Sci. U. S. A."},{"issue":"9","key":"2026081707105279000_cit25","doi-asserted-by":"publisher","first-page":"1722","DOI":"10.3390\/v13091722","article-title":"Substrate\nspecificity of sars-cov-2 nsp10-nsp16 methyltransferase","volume":"13","author":"Benoni","year":"2021","journal-title":"Viruses"},{"issue":"1","key":"2026081707105279000_cit26","doi-asserted-by":"publisher","first-page":"3717","DOI":"10.1038\/s41467-020-17495-9","article-title":"Structural analysis\nof the SARS-CoV-2 methyltransferase complex involved in RNA cap creation\nbound to sinefungin","volume":"11","author":"Krafcikova","year":"2020","journal-title":"Nat. Commun."},{"issue":"5","key":"2026081707105279000_cit27","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002059","article-title":"Crystal Structure and\nFunctional Analysis of the SARS-Coronavirus RNA Cap 2\u2032-O-Methyltransferase\nnsp10\/nsp16 Complex","volume":"7","author":"Decroly","year":"2011","journal-title":"PLOS Pathogens"},{"issue":"10","key":"2026081707105279000_cit28","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002294","article-title":"Biochemical\nand Structural Insights into the Mechanisms of SARS Coronavirus RNA\nRibose 2\u2032-O-Methylation by nsp16\/nsp10 Protein Complex","volume":"7","author":"Chen","year":"2011","journal-title":"PLOS Pathogens"},{"issue":"14","key":"2026081707105279000_cit29","doi-asserted-by":"publisher","first-page":"2880","DOI":"10.1016\/j.bpj.2021.03.024","article-title":"SARS-CoV-2\nNsp16 activation mechanism\nand a cryptic pocket with pan-coronavirus antiviral potential","volume":"120","author":"Vithani","year":"2021","journal-title":"Biophys. J."},{"issue":"5","key":"2026081707105279000_cit30","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02217-16","article-title":"Binding of the Methyl\nDonor S-Adenosyl-l-Methionine\nto Middle East Respiratory Syndrome Coronavirus 2\u2019-O-Methyltransferase\nnsp16 Promotes Recruitment of the Allosteric Activator nsp10","volume":"91","author":"Aouadi","year":"2017","journal-title":"J. Virol"},{"issue":"4","key":"2026081707105279000_cit31","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000863","article-title":"In Vitro Reconstitution\nof SARS-Coronavirus\nmRNA Cap Methylation","volume":"6","author":"Bouvet","year":"2010","journal-title":"PLOS Pathogens"},{"issue":"1","key":"2026081707105279000_cit32","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1038\/s41392-022-00884-5","article-title":"Structural\nbiology of SARS-CoV-2: open the door for novel therapies","volume":"7","author":"Yan","year":"2022","journal-title":"Signal Transduction and Targeted Therapy"},{"issue":"4","key":"2026081707105279000_cit33","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0250019","article-title":"High-throughput\nscreening of the ReFRAME, Pandemic\nBox, and COVID Box drug repurposing libraries against SARS-CoV-2 nsp15\nendoribonuclease to identify small-molecule inhibitors of viral activity","volume":"16","author":"Choi","year":"2021","journal-title":"PLoS One"},{"key":"2026081707105279000_cit34","doi-asserted-by":"publisher","first-page":"1918","DOI":"10.1021\/acsinfecdis.3c00203","article-title":"Discovery of a Druggable, Cryptic Pocket in SARS-CoV-2 nsp16 Using\nAllosteric Inhibitors","volume":"9","author":"Inniss","year":"2023","journal-title":"ACS Infectious Diseases"},{"issue":"7","key":"2026081707105279000_cit35","doi-asserted-by":"publisher","first-page":"1596","DOI":"10.1002\/pro.3873","article-title":"Crystal structure of Nsp15 endoribonuclease\nNendoU\nfrom SARS-CoV-2","volume":"29","author":"Kim","year":"2020","journal-title":"Protein Sci."},{"key":"2026081707105279000_cit36","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1007\/978-1-4939-0354-2_7","article-title":"Protein Production for Structural\nGenomics Using E. coli Expression","volume-title":"Structural\nGenomics and Drug Discovery: Methods and Protocols","author":"Makowska-Grzyska","year":"2014"},{"issue":"16","key":"2026081707105279000_cit37","doi-asserted-by":"publisher","first-page":"11195","DOI":"10.1021\/acs.analchem.0c01657","article-title":"Simple and\nminimally invasive SID devices for native mass spectrometry","volume":"92","author":"Snyder","year":"2020","journal-title":"Anal. Chem."},{"issue":"8","key":"2026081707105279000_cit38","doi-asserted-by":"publisher","first-page":"4370","DOI":"10.1021\/acs.analchem.5b00140","article-title":"Bayesian deconvolution\nof mass and ion mobility spectra:\nFrom binary interactions to polydisperse ensembles","volume":"87","author":"Marty","year":"2015","journal-title":"Anal. Chem."},{"issue":"1","key":"2026081707105279000_cit39","doi-asserted-by":"publisher","first-page":"118","DOI":"10.1007\/s13361-018-1951-9","article-title":"MetaUniDec:\nHigh-Throughput Deconvolution\nof Native Mass Spectra","volume":"30","author":"Reid","year":"2019","journal-title":"J. Am. Soc. Mass Spectrom."},{"key":"2026081707105279000_cit40","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1016\/j.softx.2015.06.001","article-title":"GROMACS: High performance\nmolecular simulations through multi-level parallelism from laptops\nto supercomputers","volume":"1\u20132","author":"Abraham","year":"2015","journal-title":"SoftwareX"},{"issue":"8","key":"2026081707105279000_cit41","doi-asserted-by":"publisher","first-page":"3696","DOI":"10.1021\/acs.jctc.5b00255","article-title":"ff14SB: Improving\nthe Accuracy of\nProtein Side Chain and Backbone Parameters from ff99SB","volume":"11","author":"Maier","year":"2015","journal-title":"J. Chem. Theory Comput."},{"issue":"2","key":"2026081707105279000_cit42","doi-asserted-by":"publisher","first-page":"926","DOI":"10.1063\/1.445869","article-title":"Comparison\nof simple potential functions for simulating liquid water","volume":"79","author":"Jorgensen","year":"1983","journal-title":"J. Chem. Phys."},{"issue":"30","key":"2026081707105279000_cit43","doi-asserted-by":"publisher","first-page":"9020","DOI":"10.1021\/jp8001614","article-title":"Determination of Alkali and Halide\nMonovalent Ion Parameters for Use in Explicitly Solvated Biomolecular\nSimulations","volume":"112","author":"Joung","year":"2008","journal-title":"J. Phys. Chem. B"},{"issue":"8","key":"2026081707105279000_cit44","doi-asserted-by":"publisher","first-page":"3684","DOI":"10.1063\/1.448118","article-title":"Molecular\ndynamics with coupling to an external bath","volume":"81","author":"Berendsen","year":"1984","journal-title":"J. Chem. Phys."},{"issue":"12","key":"2026081707105279000_cit45","doi-asserted-by":"publisher","first-page":"7182","DOI":"10.1063\/1.328693","article-title":"Polymorphic transitions\nin single crystals: A new molecular\ndynamics method","volume":"52","author":"Parrinello","year":"1981","journal-title":"J. Appl. Phys."},{"issue":"12","key":"2026081707105279000_cit46","doi-asserted-by":"publisher","first-page":"10089","DOI":"10.1063\/1.464397","article-title":"Particle mesh\nEwald: An N\u00b7log\u00ad(N)\nmethod for Ewald sums in large systems","volume":"98","author":"Darden","year":"1993","journal-title":"J. Chem.\nPhys."},{"issue":"1","key":"2026081707105279000_cit47","doi-asserted-by":"publisher","first-page":"3287","DOI":"10.1038\/s41467-021-23594-y","article-title":"A metal ion orients\nSARS-CoV-2 mRNA to ensure accurate 2\u2032-O methylation of its\nfirst nucleotide","volume":"12","author":"Viswanathan","year":"2021","journal-title":"Nat. Commun."},{"issue":"1","key":"2026081707105279000_cit48","doi-asserted-by":"publisher","first-page":"3718","DOI":"10.1038\/s41467-020-17496-8","article-title":"Structural basis of\nRNA cap modification by\nSARS-CoV-2","volume":"11","author":"Viswanathan","year":"2020","journal-title":"Nat. Commun."},{"issue":"51","key":"2026081707105279000_cit49","doi-asserted-by":"publisher","first-page":"14002","DOI":"10.1002\/anie.201403741","article-title":"Mass Spectrometry\nQuantifies Protein Interactions\ue5f8From\nMolecular Chaperones to Membrane Porins","volume":"53","author":"Hopper","year":"2014","journal-title":"Angew.\nChem., Int. Ed."},{"key":"2026081707105279000_cit50","doi-asserted-by":"publisher","first-page":"963","DOI":"10.1021\/acscentsci.2c00215","article-title":"Quantifying\nBiomolecular Interactions Using Slow Mixing\nMode (SLOMO) Nanoflow ESI-MS","volume":"8","author":"Bui","year":"2022","journal-title":"ACS Central Science"},{"issue":"19","key":"2026081707105279000_cit51","doi-asserted-by":"publisher","first-page":"7375","DOI":"10.3390\/ijms21197375","article-title":"Crystal\nStructure of\nNon-Structural Protein 10 from Severe Acute Respiratory Syndrome Coronavirus-2","volume":"21","author":"Rogstam","year":"2020","journal-title":"International Journal of Molecular Sciences"},{"issue":"1","key":"2026081707105279000_cit52","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1038\/s41392-020-00241-4","article-title":"Crystal structure of\nSARS-CoV-2 nsp10\/nsp16 2\u2032-O-methylase\nand its implication on antiviral drug design","volume":"5","author":"Lin","year":"2020","journal-title":"Signal Transduction and Targeted Therapy"},{"issue":"7","key":"2026081707105279000_cit53","doi-asserted-by":"publisher","first-page":"1149","DOI":"10.1007\/s13361-019-02201-x","article-title":"Top or Middle? Up\nor Down? Toward a Standard Lexicon for Protein Top-Down and Allied\nMass Spectrometry Approaches","volume":"30","author":"Lermyte","year":"2019","journal-title":"J. Am. Soc. Mass\nSpectrom."},{"issue":"18","key":"2026081707105279000_cit54","doi-asserted-by":"publisher","first-page":"4336","DOI":"10.1002\/anie.201108700","article-title":"Protein subunits\nreleased by surface\ncollisions of noncovalent complexes: nativelike compact structures\nrevealed by ion mobility mass spectrometry","volume":"51","author":"Zhou","year":"2012","journal-title":"Angew. Chem., Int. Ed. Engl."},{"issue":"1","key":"2026081707105279000_cit55","doi-asserted-by":"publisher","first-page":"190","DOI":"10.1021\/acs.analchem.8b05071","article-title":"Surface-Induced\nDissociation: An Effective Method for\nCharacterization of Protein Quaternary Structure","volume":"91","author":"Stiving","year":"2019","journal-title":"Anal. Chem."},{"issue":"5","key":"2026081707105279000_cit56","doi-asserted-by":"publisher","first-page":"1353","DOI":"10.1039\/c2an36525a","article-title":"Impact of charge\nstate on gas-phase\nbehaviors of noncovalent protein complexes in collision induced dissociation\nand surface induced dissociation","volume":"138","author":"Zhou","year":"2013","journal-title":"Analyst"},{"issue":"7","key":"2026081707105279000_cit57","doi-asserted-by":"publisher","first-page":"3429","DOI":"10.1021\/ja2096859","article-title":"Charge-State\nDependent Compaction and Dissociation of Protein Complexes: Insights\nfrom Ion Mobility and Molecular Dynamics","volume":"134","author":"Hall","year":"2012","journal-title":"J.\nAm. Chem. 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