{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T12:27:29Z","timestamp":1773059249030,"version":"3.50.1"},"reference-count":50,"publisher":"American Chemical Society (ACS)","issue":"11","license":[{"start":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T00:00:00Z","timestamp":1759363200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T00:00:00Z","timestamp":1759363200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T00:00:00Z","timestamp":1759363200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["75N93022C00009"],"award-info":[{"award-number":["75N93022C00009"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["R01AG063659"],"award-info":[{"award-number":["R01AG063659"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008091","name":"University of Rochester","doi-asserted-by":"publisher","award":["UM1-AI0169511"],"award-info":[{"award-number":["UM1-AI0169511"]}],"id":[{"id":"10.13039\/100008091","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Am. Soc. Mass Spectrom."],"published-print":{"date-parts":[[2025,11,5]]},"DOI":"10.1021\/jasms.5c00198","type":"journal-article","created":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T19:34:13Z","timestamp":1759433653000},"page":"2445-2453","source":"Crossref","is-referenced-by-count":1,"title":["Proteoform Characterization of HIV-1 Broadly Neutralizing Antibody PGT 121.414.LS Product Through Middle-Up and Bottom-Up Proteomics for Clinical Support"],"prefix":"10.1021","volume":"36","author":[{"given":"Connor E.","family":"Gould","sequence":"first","affiliation":[{"name":"Department of Chemistry, Chemistry Instrument Center, University at Buffalo, State University of New York, Natural Sciences Complex, Buffalo, New York 14260-3000, United States"},{"name":"Center for Integrated Global Biomedical Sciences, Department of Pharmacy Practice, School of Pharmacy and Pharmaceutical Sciences, University at Buffalo, State University of New York, Biomedical Research Building, Main Street Campus, 330 Hayes Rd., Buffalo, New York 14214, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing","family":"Ma","sequence":"additional","affiliation":[{"name":"Center for Integrated Global Biomedical Sciences, Department of Pharmacy Practice, School of Pharmacy and Pharmaceutical Sciences, University at Buffalo, State University of New York, Biomedical Research Building, Main Street Campus, 330 Hayes Rd., Buffalo, New York 14214, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raymond","family":"Cha","sequence":"additional","affiliation":[{"name":"Center for Integrated Global Biomedical Sciences, Department of Pharmacy Practice, School of Pharmacy and Pharmaceutical Sciences, University at Buffalo, State University of New York, Biomedical Research Building, Main Street Campus, 330 Hayes Rd., Buffalo, New York 14214, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valerie A.","family":"Frerichs","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Chemistry Instrument Center, University at Buffalo, State University of New York, Natural Sciences Complex, Buffalo, New York 14260-3000, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4764-8168","authenticated-orcid":true,"given":"Alan E.","family":"Friedman","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Chemistry Instrument Center, University at Buffalo, State University of New York, Natural Sciences Complex, Buffalo, New York 14260-3000, United States"},{"name":"Department of Chemical and Biological Engineering, University at Buffalo, State University of New York, Furnas Hall, Buffalo, New York 14260-4200, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robin","family":"DiFrancesco","sequence":"additional","affiliation":[{"name":"Center for Integrated Global Biomedical Sciences, Department of Pharmacy Practice, School of Pharmacy and Pharmaceutical Sciences, University at Buffalo, State University of New York, Biomedical Research Building, Main Street Campus, 330 Hayes Rd., Buffalo, New York 14214, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gene D.","family":"Morse","sequence":"additional","affiliation":[{"name":"Center for Integrated Global Biomedical Sciences, Department of Pharmacy Practice, School of Pharmacy and Pharmaceutical Sciences, University at Buffalo, State University of New York, Biomedical Research Building, Main Street Campus, 330 Hayes Rd., Buffalo, New York 14214, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2556-8511","authenticated-orcid":true,"given":"Troy D.","family":"Wood","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Chemistry Instrument Center, University at Buffalo, State University of New York, Natural Sciences Complex, Buffalo, New York 14260-3000, United States"},{"name":"Center for Integrated Global Biomedical Sciences, Department of Pharmacy Practice, School of Pharmacy and Pharmaceutical Sciences, University at Buffalo, State University of New York, Biomedical Research Building, Main Street Campus, 330 Hayes Rd., Buffalo, New York 14214, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"316","published-online":{"date-parts":[[2025,10,2]]},"reference":[{"key":"ref1\/cit1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10373"},{"key":"ref2\/cit2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2012.08.012"},{"key":"ref3\/cit3","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12075"},{"key":"ref4\/cit4","doi-asserted-by":"publisher","DOI":"10.1007\/s11904-016-0299-7"},{"key":"ref5\/cit5","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-04797-9"},{"key":"ref6\/cit6","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2020.1713707"},{"key":"ref7\/cit7","doi-asserted-by":"publisher","DOI":"10.1126\/science.1187659"},{"key":"ref8\/cit8","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-041015-055515"},{"key":"ref9\/cit9","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-022-01815-1"},{"key":"ref10\/cit10","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiac041"},{"key":"ref11\/cit11","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-021-01509-0"},{"key":"ref12\/cit12","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2020-042247"},{"key":"ref13\/cit13","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-021-01509-0"},{"key":"ref14\/cit14","doi-asserted-by":"publisher","DOI":"10.1016\/j.plabm.2021.e00260"},{"key":"ref15\/cit15","doi-asserted-by":"publisher","DOI":"10.1080\/19420862.2020.1795492"},{"key":"ref16\/cit16","doi-asserted-by":"publisher","DOI":"10.1039\/C9AY02087J"},{"key":"ref17\/cit17","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2018.02.040"},{"key":"ref18\/cit18","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.7898"},{"key":"ref19\/cit19","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2025.124774"},{"key":"ref20\/cit20","doi-asserted-by":"publisher","DOI":"10.4161\/mabs.2.5.12897"},{"key":"ref21\/cit21","doi-asserted-by":"publisher","DOI":"10.1021\/ac3032355"},{"key":"ref22\/cit22","doi-asserted-by":"publisher","DOI":"10.1038\/nature13612"},{"key":"ref23\/cit23","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1403014"},{"key":"ref24\/cit24","doi-asserted-by":"publisher","DOI":"10.4161\/mabs.3.6.17922"},{"key":"ref25\/cit25","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00796-21"},{"key":"ref26\/cit26","doi-asserted-by":"publisher","DOI":"10.1016\/j.xphs.2018.08.012"},{"key":"ref27\/cit27","unstructured":"WHO.\n                      Guideline for the production and quality\ncontrol of monoclonal\nantibodies and related products intended for medicinal use\n                      ; World Health Organization, 2021."},{"key":"ref28\/cit28","doi-asserted-by":"publisher","DOI":"10.1021\/jasms.0c00036"},{"key":"ref29\/cit29","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2016.05.017"},{"key":"ref30\/cit30","doi-asserted-by":"publisher","DOI":"10.1002\/mas.20190"},{"key":"ref31\/cit31","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.0c02780"},{"key":"ref32\/cit32","doi-asserted-by":"publisher","DOI":"10.1002\/mas.20129"},{"key":"ref33\/cit33","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2013.04.022"},{"key":"ref34\/cit34","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms14954"},{"key":"ref35\/cit35","doi-asserted-by":"publisher","DOI":"10.4161\/mabs.2.3.11718"},{"key":"ref36\/cit36","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-019-01942-8"},{"key":"ref37\/cit37","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.2c02630"},{"key":"ref38\/cit38","doi-asserted-by":"publisher","DOI":"10.1007\/s13361-019-02296-2"},{"key":"ref39\/cit39","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.227"},{"key":"ref40\/cit40","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5b00140"},{"key":"ref41\/cit41","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth1060"},{"key":"ref42\/cit42","doi-asserted-by":"publisher","DOI":"10.1007\/s10337-017-3424-x"},{"key":"ref43\/cit43","doi-asserted-by":"publisher","DOI":"10.1021\/ac00190a023"},{"key":"ref44\/cit44","volume-title":"The NIST Glyco Mass Calculator","author":"De Leoz M. L. A.","year":"2015"},{"key":"ref45\/cit45","doi-asserted-by":"crossref","DOI":"10.1385\/1592598900","volume-title":"The proteomics protocols handbook","author":"Walker J. M.","year":"2005"},{"key":"ref46\/cit46","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg563"},{"key":"ref47\/cit47","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpha.2019.11.008"},{"key":"ref48\/cit48","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003342"},{"key":"ref49\/cit49","doi-asserted-by":"publisher","DOI":"10.1111\/imr.13407"},{"key":"ref50\/cit50","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.8b02637"}],"container-title":["Journal of the American Society for Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/jasms.5c00198","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/jasms.5c00198","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T09:12:13Z","timestamp":1762333933000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/doi\/10.1021\/jasms.5c00198"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,2]]},"references-count":50,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2025,11,5]]}},"alternative-id":["10.1021\/jasms.5c00198"],"URL":"https:\/\/doi.org\/10.1021\/jasms.5c00198","relation":{},"ISSN":["1044-0305","1879-1123"],"issn-type":[{"value":"1044-0305","type":"print"},{"value":"1879-1123","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,2]]}}}