{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T15:49:30Z","timestamp":1791388170290,"version":"4.3.3"},"reference-count":12,"publisher":"American Chemical Society (ACS)","issue":"10","license":[{"start":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:00:00Z","timestamp":1787616000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["U01CA294527"],"award-info":[{"award-number":["U01CA294527"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014989","name":"Chan Zuckerberg Initiative","doi-asserted-by":"publisher","award":["2021-240339"],"award-info":[{"award-number":["2021-240339"]}],"id":[{"id":"10.13039\/100014989","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014989","name":"Chan Zuckerberg Initiative","doi-asserted-by":"publisher","award":["2022-309518"],"award-info":[{"award-number":["2022-309518"]}],"id":[{"id":"10.13039\/100014989","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["R01AG078803"],"award-info":[{"award-number":["R01AG078803"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Vanderbilt Interdisciplinary Training Program in Alzheimer?s Disease","award":["T32-AG058524"],"award-info":[{"award-number":["T32-AG058524"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Triacylglycerols (TAGs) are essential neutral lipids with critical roles in cellular function and disease. However, spatial characterization of these chemical species remains difficult because neutral molecules often exhibit low ionization efficiency with common mass spectrometry-based imaging strategies, such as matrix-assisted laser desorption\/ionization imaging mass spectrometry (MALDI IMS). Here, we assess how ammonium formate (AmF) or sodium carbonate buffer solution (CBS) tissue washes, combined with varying primary laser power, affect TAG sensitivity with and without MALDI-2 postionization. We found that Na+ doping using Na+ CBS washes combined with MALDI-2 at 31% total laser power was optimal for both signal intensity and molecular coverage. Additionally, Na+ CBS-washed tissues enabled MALDI-2 to be performed using lower laser powers, making this method compatible with sequential microscopy experiments as part of multimodal workflows. Applying this strategy to diseased brain tissue enabled spatial mapping of TAGs linked to Alzheimer\u2019s disease. This approach balances sensitivity and tissue preservation, providing a robust platform for multimodal spatial lipidomic studies across diverse tissues and disease contexts.<\/jats:p>","DOI":"10.1021\/jasms.6c00227","type":"journal-article","created":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T22:01:32Z","timestamp":1788213692000},"page":"2618-2622","source":"Crossref","is-referenced-by-count":0,"title":["Optimized Sodium-Enhanced\nMALDI-2 Enables Multimodal\nImaging of Triacylglycerols"],"prefix":"10.1021","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-5441-2153","authenticated-orcid":true,"given":"Kameron R.","family":"Molloy","sequence":"first","affiliation":[{"name":"Vanderbilt University , , , ,","place":["Nashville, Tennessee, United States, 37235"]},{"name":"Vanderbilt University , , , ,","place":["Nashville, Tennessee, United States, 37235"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cody R.","family":"Marshall","sequence":"additional","affiliation":[{"name":"Vanderbilt University , , , ,","place":["Nashville, Tennessee, United States, 37235"]},{"name":"Chemical and Physical Biology Program, Vanderbilt University School of Medicine , , ,","place":["Nashville, Tennessee, United States, 37240"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1731-3666","authenticated-orcid":true,"given":"Martin","family":"Dufresne","sequence":"additional","affiliation":[{"name":"Vanderbilt University , , , ,","place":["Nashville, Tennessee, United States, 37235"]},{"name":"Vanderbilt University , , , ,","place":["Nashville, Tennessee, United States, 37232"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1884-6405","authenticated-orcid":true,"given":"Lukasz G.","family":"Migas","sequence":"additional","affiliation":[{"name":"Vanderbilt University , , , ,","place":["Nashville, Tennessee, United States, 37235"]},{"name":"Delft University of Technology , , ,","place":["Delft, Netherlands, 2628"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew S.","family":"Schrag","sequence":"additional","affiliation":[{"name":"Vanderbilt University School of Medicine , , , ,","place":["Nashville, Tennessee, United States, 37232"]},{"name":"Vanderbilt University , , , ,","place":["Nashville, Tennessee, United States, 37240"]},{"name":"Vanderbilt University Medical Center , , , ,","place":["Nashville, Tennessee, United States, 37232"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2232-7130","authenticated-orcid":true,"given":"Raf","family":"Van de Plas","sequence":"additional","affiliation":[{"name":"Vanderbilt University , , , ,","place":["Nashville, Tennessee, United States, 37235"]},{"name":"Delft University of Technology , , ,","place":["Delft, Netherlands, 2628"]},{"name":"Vanderbilt University , , , ,","place":["Nashville, Tennessee, United States, 37205"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9198-5498","authenticated-orcid":true,"given":"Jeffrey M.","family":"Spraggins","sequence":"additional","affiliation":[{"name":"Vanderbilt University , , , ,","place":["Nashville, Tennessee, United States, 37235"]},{"name":"Vanderbilt University , , , ,","place":["Nashville, Tennessee, United States, 37235"]},{"name":"Chemical and Physical Biology Program, Vanderbilt University School of Medicine , , ,","place":["Nashville, Tennessee, United States, 37240"]},{"name":"Vanderbilt University , , , ,","place":["Nashville, Tennessee, United States, 37232"]},{"name":"Vanderbilt University , , , ,","place":["Nashville, Tennessee, United States, 37205"]},{"name":"Vanderbilt University Medical Center , , , ,","place":["Nashville, Tennessee, United States, 37205"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,25]]},"reference":[{"issue":"10","key":"2026100710070261900_cit1","doi-asserted-by":"publisher","first-page":"2591","DOI":"10.3390\/cells10102591","article-title":"Lipid Profiling\nof Alzheimer\u2019s Disease Brain Highlights Enrichment in Glycerol(Phospho)Lipid,\nand Sphingolipid Metabolism","volume":"10","author":"Akyol","year":"2021","journal-title":"Cells"},{"issue":"4","key":"2026100710070261900_cit2","doi-asserted-by":"publisher","first-page":"397","DOI":"10.1016\/j.stem.2015.08.001","article-title":"Aberrant\nLipid Metabolism in the Forebrain Niche Suppresses Adult Neural Stem\nCell Proliferation in an Animal Model of Alzheimer\u2019s Disease","volume":"17","author":"Hamilton","year":"2015","journal-title":"Cell Stem Cell"},{"issue":"11","key":"2026100710070261900_cit3","doi-asserted-by":"publisher","first-page":"776","DOI":"10.1016\/j.bbalip.2011.06.019","article-title":"Glycerolipid and\nCholesterol Ester\nAnalyses in Biological Samples by Mass Spectrometry","volume":"1811","author":"Murphy","year":"2011","journal-title":"Biochim. Biophys. Acta, Mol. Cell Biol. Lipids"},{"issue":"10","key":"2026100710070261900_cit4","doi-asserted-by":"publisher","first-page":"2277","DOI":"10.1007\/s00216-019-02290-3","article-title":"Evaluation\nof Lipid Coverage and\nHigh Spatial Resolution MALDI-Imaging Capabilities of Oversampling\nCombined with Laser Post-Ionisation","volume":"412","author":"Bowman","year":"2020","journal-title":"Anal. Bioanal.\nChem."},{"key":"2026100710070261900_cit5","doi-asserted-by":"publisher","first-page":"2213","DOI":"10.1021\/jasms.5c00202","article-title":"High-Specificity and Sensitivity Imaging of Neutral\nLipids Using Salt-Enhanced MALDI TIMS","volume":"36","author":"Molloy","year":"2025","journal-title":"J. Am.\nSoc. Mass Spectrom."},{"issue":"20","key":"2026100710070261900_cit6","doi-asserted-by":"publisher","first-page":"12928","DOI":"10.1021\/acs.analchem.9b02974","article-title":"Combining Salt Doping\nand Matrix Sublimation for High Spatial Resolution MALDI Imaging Mass\nSpectrometry of Neutral Lipids","volume":"91","author":"Dufresne","year":"2019","journal-title":"Anal. Chem."},{"issue":"11","key":"2026100710070261900_cit7","doi-asserted-by":"publisher","first-page":"6018","DOI":"10.1021\/acs.analchem.6b01141","article-title":"Sodium-Doped\nGold-Assisted Laser\nDesorption Ionization for Enhanced Imaging Mass Spectrometry of Triacylglycerols\nfrom Thin Tissue Sections","volume":"88","author":"Dufresne","year":"2016","journal-title":"Anal. Chem."},{"key":"2026100710070261900_cit8","doi-asserted-by":"crossref","unstructured":"Migas, L.\n          \n          IMS Data Processing\nV1, 2023.","DOI":"10.17504\/protocols.io.e6nvwjyj9lmk\/v1"},{"issue":"6","key":"2026100710070261900_cit9","doi-asserted-by":"publisher","first-page":"905","DOI":"10.1002\/rcm.3444","article-title":"mMass Data Miner: An Open Source Alternative for Mass Spectrometric Data\nAnalysis","volume":"22","author":"Strohalm","year":"2008","journal-title":"Rapid Commun. Mass Spectrom."},{"key":"2026100710070261900_cit10","doi-asserted-by":"publisher","first-page":"D527","DOI":"10.1093\/nar\/gkl838","article-title":"LIPID MAPS Structure\nDatabase","volume":"35","author":"Sud","year":"2007","journal-title":"Nucleic Acids Res."},{"issue":"6231","key":"2026100710070261900_cit11","doi-asserted-by":"publisher","first-page":"211","DOI":"10.1126\/science.aaa1051","article-title":"Mass Spectrometry Imaging with Laser-Induced\nPostionization","volume":"348","author":"Soltwisch","year":"2015","journal-title":"Science"},{"issue":"1","key":"2026100710070261900_cit12","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1007\/s00216-011-4814-9","article-title":"MALDI Imaging Mass\nSpectrometry of Lipids by Adding\nLithium Salts to the Matrix Solution","volume":"401","author":"Cerruti","year":"2011","journal-title":"Anal.\nBioanal. Chem."}],"container-title":["Journal of the American\nSociety for Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/jasms.6c00227","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/jamsef\/article-pdf\/37\/10\/2618\/67394000\/jasms.6c00227.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/jamsef\/article-pdf\/37\/10\/2618\/67394000\/jasms.6c00227.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T14:49:36Z","timestamp":1791384576000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/jamsef\/article\/37\/10\/2618\/5328300\/Optimized-Sodium-Enhanced-MALDI-2-Enables"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,25]]},"references-count":12,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2026,8,25]]},"published-print":{"date-parts":[[2026,10,7]]}},"URL":"https:\/\/doi.org\/10.1021\/jasms.6c00227","relation":{"has-preprint":[{"id-type":"doi","id":"10.26434\/chemrxiv.15002386\/v1","asserted-by":"object"}]},"ISSN":["1044-0305","1879-1123"],"issn-type":[{"value":"1044-0305","type":"print"},{"value":"1879-1123","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,25]]}}}