{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:16:49Z","timestamp":1791307009208,"version":"4.3.1"},"reference-count":43,"publisher":"American Chemical Society (ACS)","issue":"37","license":[{"start":{"date-parts":[[2015,8,19]],"date-time":"2015-08-19T00:00:00Z","timestamp":1439942400000},"content-version":"vor","delay-in-days":365,"URL":"https:\/\/pubs.acs.org\/page\/policy\/authorchoice_termsofuse.html"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["312140"],"award-info":[{"award-number":["312140"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,9,17]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The knowledge of pectin esterification degree is of primary importance to predict gelling and other properties of pectin from different sources. This paper reports the development of a simple and rapid 1H NMR-based method for the simultaneous quantitative determination of methylation, acetylation, and feruloylation degree of pectin isolated from various food sources. Pectin esters are hydrolyzed in NaOH\/D2O, and the obtained methanol, acetic acid, and ferulic acid are directly measured by 1H NMR. High accuracy, repeatability, and reproducibility of the method were obtained, and the analysis time is reduced as compared to conventional chromatography- or titration-based methods.<\/jats:p>","DOI":"10.1021\/jf502679s","type":"journal-article","created":{"date-parts":[[2014,8,19]],"date-time":"2014-08-19T13:05:55Z","timestamp":1408453555000},"page":"9081-9087","source":"Crossref","is-referenced-by-count":118,"title":["Simple\nand Validated Quantitative 1H NMR\nMethod for the Determination of Methylation, Acetylation, and Feruloylation\nDegree of Pectin"],"prefix":"10.1021","volume":"62","author":[{"given":"Judith","family":"Mu\u0308ller-Maatsch","sequence":"first","affiliation":[{"name":"University of Parma , , ,","place":["Parma, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Augusta","family":"Caligiani","sequence":"additional","affiliation":[{"name":"University of Parma , , ,","place":["Parma, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tullia","family":"Tedeschi","sequence":"additional","affiliation":[{"name":"University of Parma , , ,","place":["Parma, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathy","family":"Elst","sequence":"additional","affiliation":[{"name":"Flemish Institute for Technological Research , ,","place":["Mol, Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Sforza","sequence":"additional","affiliation":[{"name":"University of Parma , , ,","place":["Parma, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2014,8,19]]},"reference":[{"key":"2026090313565385500_cit1","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1016\/S0031-9422(01)00113-3","article-title":"Pectins: structure, biosynthesis,\nand oligogalacturonide-related signaling","volume":"57","author":"Ridley","year":"2001","journal-title":"Phytochemistry"},{"key":"2026090313565385500_cit2","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1007\/s11224-009-9442-z","article-title":"Pectin, a versatile\npolysaccharide present in plant cell walls","volume":"20","author":"Voragen","year":"2009","journal-title":"Struct. 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Food Chem."},{"key":"2026090313565385500_cit38","article-title":"Isolation of plant cell walls\nand fractionation of cell wall polysaccharides","volume-title":"Current Protocols in Food Analytical Chemistry","author":"Melton","year":"1991"},{"key":"2026090313565385500_cit39","article-title":"Determination of the uronic\nacid content of plant cell walls using a colorimetric assay","volume-title":"Current Protocols in Food Analytical Chemistry","author":"Melton","year":"1991"},{"key":"2026090313565385500_cit40","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.aca.2006.12.016","article-title":"Identification\nand\nquantification of the main organic components of vinegars by high\nresolution 1H NMR spectroscopy","volume":"585","author":"Caligiani","year":"2007","journal-title":"Anal.\nChim. Acta"},{"key":"2026090313565385500_cit41","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.ab.2008.09.053","article-title":"Comparison of different\napproaches for evaluation of the detection and quantitation limits\nof a purity method: a case study using a capillary isoelectrofocusing\nmethod for a monoclonal antibody","volume":"385","author":"Apostol","year":"2009","journal-title":"Anal. Biochem."},{"key":"2026090313565385500_cit42","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/0144-8617(94)90024-8","article-title":"Structure\nand properties of the polysaccharides\nfrom pea hulls. Part 1: Chemical extraction and fractionation of the\npolysaccharides","volume":"24","author":"Weightman","year":"1994","journal-title":"Carbohydr. Polym."},{"key":"2026090313565385500_cit43","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/0008-6215(93)80007-2","article-title":"Structure and properties\nof apple and sugar-beet pectins\nextracted by chelating agents","volume":"244","author":"Renard","year":"1993","journal-title":"Carbohydr. 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