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Chin. Chem. Soc."},{"key":"jm020548bb00002\/jm020548bb00002_1","first-page":"540","volume":"62","author":"Alali F. Q.","year":"1999","journal-title":"J. Nat. Prod."},{"key":"jm020548bb00003\/jm020548bb00003_1","doi-asserted-by":"crossref","first-page":"2106","DOI":"10.1021\/jm9700169","volume":"40","author":"Oberlies N. H.","year":"1997","journal-title":"J. Med. Chem."},{"key":"jm020548bb00004\/jm020548bb00004_1","first-page":"452","volume":"1365","author":"Miyoshi H.","year":"1998","journal-title":"Biochim. Biophys. Acta"},{"key":"jm020548bb00005\/jm020548bb00005_1","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1002\/ps.2780330405","volume":"33","author":"Londershausen M.","year":"1991","journal-title":"Pestic. Sci."},{"key":"jm020548bb00006\/jm020548bb00006_1","doi-asserted-by":"crossref","first-page":"1120","DOI":"10.1016\/0024-3205(93)90547-G","volume":"53","author":"Ahammadsahib K. I.","year":"1993","journal-title":"Life Sci."},{"key":"jm020548bb00007\/jm020548bb00007_1","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1042\/bst0220230","volume":"22","author":"Hollingworth R. M.","year":"1994","journal-title":"Biochem. Soc. Trans."},{"key":"jm020548bb00008\/jm020548bb00008_1","first-page":"348","volume":"56","author":"Morre D. J.","year":"1995","journal-title":"Life Sci."},{"key":"jm020548bb00009\/jm020548bb00009_1","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/S0304-3835(97)04716-2","volume":"115","author":"Oberlies N. H.","year":"1997","journal-title":"Cancer Lett."},{"key":"jm020548bb00010\/jm020548bb00010_1","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1006\/phrs.1998.0314","volume":"37","author":"Shimada H.","year":"1998","journal-title":"Pharmacol. Res."},{"key":"jm020548bb00011\/jm020548bb00011_1","doi-asserted-by":"crossref","first-page":"866","DOI":"10.1021\/bi972348l","volume":"37","author":"Shimada H.","year":"1998","journal-title":"Biochemistry"},{"key":"jm020548bb00012\/jm020548bb00012_1","first-page":"1331","volume":"69","author":"Chih H. W.","year":"2001","journal-title":"Life Sci."},{"key":"jm020548bb00013\/jm020548bb00013_1","first-page":"22","volume":"16","author":"Wu S. N.","year":"2003","journal-title":"Chem. Res. Toxicol."},{"key":"jm020548bb00014\/jm020548bb00014_1","unstructured":"Annonaceous acetogenins were isolated from Formosan Annonaceous plants in Dr. Yang-Chang Wu's laboratory in Taiwan, includingAnnona muricata,A. montana,A. atemoya,A. reticulata, andRollinia mucosa. Compounds1\u221218,22,23, and24were isolated fromA. muricata. Compounds14,15,18,20, and25were isolated fromA. montana. Compounds19,22,26,27, and28were isolated fromA.reticulata. Compounds19and21were isolated fromA. atemoya. Compounds19and27were isolated fromR.mucosa. Among them,1\u22127,9,10,20,23, and24were published as new compounds. All of the Annonaceous acetogenins were repurified by reversed-phase HPLC before bioevaluation. (A. muricataI:\u2009J. Nat. Prod.2001,64, 925\u2212931.A. muricataII:\u2009J. Nat. Prod.2002,65, 470\u2212475.A.muricataIII:\u2009Planta Med.2003,69, 241\u2212246;A. montana:\u2009Heterocycles1994,38, 1475\u22121478;A. reticulataI:\u2009Heterocycles1992,34, 667\u2212674.A.reticulataII:\u2009J. Nat. Prod.1993,56, 1688\u22121694;A. atemoya:\u2009Phytochemistry1999,51, 883\u2212889;R. mucosa:\u2009J. Nat. Prod.1999,62, 1613\u22121617.)"},{"key":"jm020548bb00015\/jm020548bb00015_1","doi-asserted-by":"crossref","first-page":"1118","DOI":"10.1093\/jnci\/82.13.1113","volume":"82","author":"Rubinstein L. V.","year":"1990","journal-title":"J. Natl. Cancer. Inst."},{"key":"jm020548bb00016\/jm020548bb00016_1","unstructured":"All stock cultures were grown in T-25 flasks. Freshly trypsinized cell suspensions were seeded in 96-well microtitre plates at densities of 1500\u22127500 cells per well with compounds added from DMSO-diluted stock. After 3 days in culture, attached cells were fixed with cold 50% trichloroacetic acid and then stained with 0.4% sulforhodamine B (SRB). The absorbency at 562 nm was measured using a microplate reader after solubilizing the bound dye. The ED50is the concentration of agent that reduced cell growth by 50% under the experimental conditions. Human tumor cell lines [KB (nasopharyngeal), HCT-8 (ileocecal), A549 (lung), MCF-7 (breast), PC-3 (prostate), HOS (bone), SK-MEL-2 (melanoma), CAKI (kidney)] were obtained from ATCC (Rockville, MD). KB-VIN (vincristine resistant KB subline) cell line was a generous gift of Dr. Y.C Cheng (Yale University). 1A9 (ovarian) and PTX10 (subline of 1A9, which has a mutated \u03b2-tubulin gene) cell lines were generous gifts of Dr. P. Ginnakakou (NCI, Bethesda, MD). All cell lines were cultured in RPMI-1640 medium supplemented with 25 mM HEPES, 0.25% sodium bicarbonate, 10% fetal bovine serum, and 100 \u03bcg\/mL kanamycin."},{"key":"jm020548bb00017\/jm020548bb00017_1","first-page":"477","volume":"52","author":"Hui Y. H.","year":"1989","journal-title":"J. Nat. Prod."},{"key":"jm020548bb00018\/jm020548bb00018_1","doi-asserted-by":"crossref","first-page":"17125","DOI":"10.1074\/jbc.272.27.17118","volume":"272","author":"Giannakakou P.","year":"1997","journal-title":"J. Biol. Chem."},{"key":"jm020548bb00019\/jm020548bb00019_1","first-page":"99","volume":"59","author":"Hopp D. C.","year":"1996","journal-title":"J. Nat. Prod."},{"key":"jm020548bb00020\/jm020548bb00020_1","volume-title":"United States Patent 5,955,497","author":"McLaughlin J. L.","year":"1999"},{"key":"jm020548bb00021\/jm020548bb00021_1","first-page":"34","volume":"62","author":"Alali F. Q.","year":"1999","journal-title":"J. Nat. 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