{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T10:30:24Z","timestamp":1787049024693,"version":"build-2736575974"},"reference-count":34,"publisher":"American Chemical Society (ACS)","issue":"6","license":[{"start":{"date-parts":[[2010,5,18]],"date-time":"2010-05-18T00:00:00Z","timestamp":1274140800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2010,5,18]],"date-time":"2010-05-18T00:00:00Z","timestamp":1274140800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2010,5,18]],"date-time":"2010-05-18T00:00:00Z","timestamp":1274140800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,6,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Semiconductor quantum dots (QDs) are used for the optical analysis of casein kinase (CK2) or the hydrolytic activity of alkaline phosphatase (ALP). Two schemes for the analysis of CK2 by a FRET-based mechanism are described. One approach involves the CK2-catalyzed phosphorylation of a serine-containing peptide (1), linked to CdSe\/ZnS QDs, with Atto-590-functionalized ATP. The second analytical method involves the specific association of the Atto-590-functionalized antibody to the phosphorylated product. The hydrolytic activity of ALP is followed by the application of phosphotyrosine (4)-modified CdSe\/ZnS QDs in the presence of tyrosinase as a secondary reporter biocatalyst. The hydrolysis of (4) yields the tyrosine units that are oxidized by O2\/tyrosinase to the respective dopaquinone product. The latter quinone units quench the QDs via an electron transfer route, leading to the optical detection of the ALP activity.<\/jats:p>","DOI":"10.1021\/nl101052f","type":"journal-article","created":{"date-parts":[[2010,5,18]],"date-time":"2010-05-18T08:12:39Z","timestamp":1274170359000},"page":"2192-2196","source":"Crossref","is-referenced-by-count":233,"title":["Probing Protein Kinase (CK2) and Alkaline Phosphatase with CdSe\/ZnS Quantum Dots"],"prefix":"10.1021","volume":"10","author":[{"given":"Ronit","family":"Freeman","sequence":"first","affiliation":[{"name":"Institute of Chemistry, The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tali","family":"Finder","sequence":"additional","affiliation":[{"name":"Institute of Chemistry, The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ron","family":"Gill","sequence":"additional","affiliation":[{"name":"Institute of Chemistry, The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Itamar","family":"Willner","sequence":"additional","affiliation":[{"name":"Institute of Chemistry, The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2010,5,18]]},"reference":[{"key":"2026081805295370500_cit1a","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1038\/308693a0","volume":"308","author":"Nishizuka","year":"1984","journal-title":"Nature."},{"key":"2026081805295370500_cit1b","doi-asserted-by":"crossref","first-page":"1912","DOI":"10.1126\/science.1075762","volume":",\u2009298","author":"Manning","year":"2002","journal-title":"Science."},{"key":"2026081805295370500_cit2","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1023\/A:1013112908734","volume":"227","author":"Wang","year":"2001","journal-title":"Mol. 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