{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T12:27:43Z","timestamp":1787056063546,"version":"build-2736575974"},"reference-count":45,"publisher":"American Chemical Society (ACS)","issue":"6","license":[{"start":{"date-parts":[[2014,5,7]],"date-time":"2014-05-07T00:00:00Z","timestamp":1399420800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2014,5,7]],"date-time":"2014-05-07T00:00:00Z","timestamp":1399420800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2014,5,7]],"date-time":"2014-05-07T00:00:00Z","timestamp":1399420800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,6,24]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>We report on in vitro self-assembly of nanometer-sized \u03b1-synuclein amyloid fibrils into well-defined micrometer-sized suprafibrillar aggregates with sheet-like or cylindrical morphology depending on the ionic strength of the solution. The cylindrical suprafibrillar structures are heavily hydrated, suggesting swollen gel-like particles. In contrast to higher order structures formed by other negatively charged biopolymers, multivalent ions are not required for the suprafibrillar aggregates to form. Their formation is induced by both mono- and divalent counterions. The self-assembly process is not mediated by protein-specific interactions but rather by the cooperative action of long-range electrostatic repulsion and short-range attraction. Understanding the mechanism driving the self-assembly might give us valuable insight into the pathological formation of fibrillar superstructures such as Lewy bodies and neurites\ue5f8distinct signatures of Parkinson\u2019s disease\ue5f8and will open the possibility to utilize the self-assembly process for the design of novel fibril-based smart nanostructured materials.<\/jats:p>","DOI":"10.1021\/nn406309c","type":"journal-article","created":{"date-parts":[[2014,5,7]],"date-time":"2014-05-07T13:36:34Z","timestamp":1399469794000},"page":"5543-5551","source":"Crossref","is-referenced-by-count":56,"title":["Self-Assembly of Protein Fibrils into Suprafibrillar Aggregates: Bridging the Nano- and Mesoscale"],"prefix":"10.1021","volume":"8","author":[{"given":"Slav A.","family":"Semerdzhiev","sequence":"first","affiliation":[{"name":"University of Twente , , PO Box 217, ,","place":["Enschede, The Netherlands, 7500 AE"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dirk R.","family":"Dekker","sequence":"additional","affiliation":[{"name":"University of Twente , , PO Box 217, ,","place":["Enschede, The Netherlands, 7500 AE"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vinod","family":"Subramaniam","sequence":"additional","affiliation":[{"name":"University of Twente , , PO Box 217, ,","place":["Enschede, The Netherlands, 7500 AE"]},{"name":"University of Twente , , PO Box 217, ,","place":["Enschede, The Netherlands, 7500 AE"]},{"name":"FOM Institute AMOLF , Science Park 104 , ,","place":["Amsterdam, The Netherlands, 1098 XG"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mireille M. A. E.","family":"Claessens","sequence":"additional","affiliation":[{"name":"University of Twente , , PO Box 217, ,","place":["Enschede, The Netherlands, 7500 AE"]},{"name":"University of Twente , , PO Box 217, ,","place":["Enschede, The Netherlands, 7500 AE"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2014,5,7]]},"reference":[{"key":"2026081807143545300_cit1","doi-asserted-by":"crossref","first-page":"3590","DOI":"10.1073\/pnas.96.7.3590","article-title":"Designing Conditions for in Vitro Formation of Amyloid Protofilaments and Fibrils","volume":"96","author":"Chiti","year":"1999","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"2026081807143545300_cit2","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1146\/annurev.biochem.75.101304.123901","article-title":"Protein Misfolding, Functional Amyloid, and Human Disease","volume":"75","author":"Chiti","year":"2006","journal-title":"Annu. Rev. 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D. v.\n          \n          Structural and Functional Insights into Interactions of Oligomeric Alpha-Synuclein with Lipid Membranes. Ph.D. Thesis, University of Twente, Enschede, 2009."}],"container-title":["ACS Nano"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/nn406309c","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/ancac3\/article-pdf\/8\/6\/5543\/14026825\/nn406309c.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/ancac3\/article-pdf\/8\/6\/5543\/14026825\/nn406309c.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T11:37:04Z","timestamp":1787053024000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/ancac3\/article\/8\/6\/5543\/1360356\/Self-Assembly-of-Protein-Fibrils-into"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,5,7]]},"references-count":45,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2014,5,7]]},"published-print":{"date-parts":[[2014,6,24]]}},"URL":"https:\/\/doi.org\/10.1021\/nn406309c","relation":{},"ISSN":["1936-0851","1936-086X"],"issn-type":[{"value":"1936-0851","type":"print"},{"value":"1936-086X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,5,7]]}}}