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We demonstrate identification of virus particles in complex samples for replication-competent wild-type vesicular stomatitis virus (VSV), defective VSV, and Ebola- and Marburg-pseudotyped VSV with high sensitivity and specificity. Size discrimination of the imaged nanoparticles (virions) allows differentiation between modified viruses having different genome lengths and facilitates a reduction in the counting of nonspecifically bound particles to achieve a limit-of-detection (LOD) of 5 \u00d7 103 pfu\/mL for the Ebola and Marburg VSV pseudotypes. We demonstrate the simultaneous detection of multiple viruses in a single sample (composed of serum or whole blood) for screening applications and uncompromised detection capabilities in samples contaminated with high levels of bacteria. By employing affinity-based capture, size discrimination, and a \u201cdigital\u201d detection scheme to count single virus particles, we show that a robust and sensitive virus\/nanoparticle sensing assay can be established for targets in complex samples. The nanoparticle microscopy system is termed the Single Particle Interferometric Reflectance Imaging Sensor (SP-IRIS) and is capable of high-throughput and rapid sizing of large numbers of biological nanoparticles on an antibody microarray for research and diagnostic applications.<\/jats:p>","DOI":"10.1021\/nn501312q","type":"journal-article","created":{"date-parts":[[2014,5,19]],"date-time":"2014-05-19T16:14:33Z","timestamp":1400516073000},"page":"6047-6055","source":"Crossref","is-referenced-by-count":93,"title":["Digital Sensing and Sizing of Vesicular Stomatitis Virus Pseudotypes in Complex Media: A Model for Ebola and Marburg Detection"],"prefix":"10.1021","volume":"8","author":[{"given":"George G.","family":"Daaboul","sequence":"first","affiliation":[{"name":"Boston University , , , ,","place":["Boston, Massachusetts, United States, 02215"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos A.","family":"Lopez","sequence":"additional","affiliation":[{"name":"Boston University , , , ,","place":["Boston, Massachusetts, United States, 02215"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jyothsna","family":"Chinnala","sequence":"additional","affiliation":[{"name":"Boston University , , , ,","place":["Boston, Massachusetts, United States, 02215"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bennett B.","family":"Goldberg","sequence":"additional","affiliation":[{"name":"Boston University , , , ,","place":["Boston, Massachusetts, United States, 02215"]},{"name":"Boston University , , , ,","place":["Boston, Massachusetts, United States, 02215"]},{"name":"Boston University , , , ,","place":["Boston, Massachusetts, United States, 02215"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John H.","family":"Connor","sequence":"additional","affiliation":[{"name":"Boston University School of Medicine , , , ,","place":["Boston, Massachusetts, United States, 02118"]},{"name":"Boston University , , , ,","place":["Boston, Massachusetts, United States, 02215"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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