{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T20:09:41Z","timestamp":1791317381071,"version":"4.3.1"},"reference-count":50,"publisher":"American Chemical Society (ACS)","issue":"7","license":[{"start":{"date-parts":[[2015,7,8]],"date-time":"2015-07-08T00:00:00Z","timestamp":1436313600000},"content-version":"vor","delay-in-days":365,"URL":"https:\/\/pubs.acs.org\/page\/policy\/authorchoice_termsofuse.html"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["ECS-9731293"],"award-info":[{"award-number":["ECS-9731293"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["ECS-9876771"],"award-info":[{"award-number":["ECS-9876771"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01-AI018306"],"award-info":[{"award-number":["R01-AI018306"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,7,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The organization and dynamics of plasma membrane components at the nanometer scale are essential for biological functions such as transmembrane signaling and endocytosis. Planarized nanoscale apertures in a metallic film are demonstrated as a means of confining the excitation light for multicolor fluorescence spectroscopy to a 55 \u00b1 10 nm beam waist. This technique provides simultaneous two-color, subdiffraction-limited fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy on planar membranes. The fabrication and implementation of this technique are demonstrated for both model membranes and live cells. Membrane-bound proteins were observed to cluster upon the addition of a multivalent cross-linker: On supported lipid bilayers, clusters of cholera toxin subunit B were formed upon cross-linking by an antibody specific for this protein; on living cells, immunoglobulin E bound to its receptor (Fc\u03b5RI) on the plasma membranes of RBL mast cells was observed to form clusters upon exposure to a trivalent antigen. The formation of membrane clusters was quantified via fluorescence intensity vs time and changes in the temporal auto- and cross-correlations above a single nanoscale aperture. The illumination profile from a single aperture is analyzed experimentally and computationally with a rim-dominated illumination profile, yielding no change in the autocorrelation dwell time with changes in aperture diameter from 60 to 250 nm. This near-field fluorescence cross-correlation methodology provides access to nanoscale details of dynamic membrane interactions and motivates further development of near-field optical methods.<\/jats:p>","DOI":"10.1021\/nn502593k","type":"journal-article","created":{"date-parts":[[2014,7,8]],"date-time":"2014-07-08T13:52:34Z","timestamp":1404827554000},"page":"7392-7404","source":"Crossref","is-referenced-by-count":27,"title":["Near-Field Fluorescence Cross-Correlation Spectroscopy on Planar Membranes"],"prefix":"10.1021","volume":"8","author":[{"given":"Christopher V.","family":"Kelly","sequence":"first","affiliation":[{"name":"Cornell University , , , ,","place":["Ithaca, New York, United States, 14853"]},{"name":"Cornell University , , , ,","place":["Ithaca, New York, United States, 14853"]},{"name":"Wayne State University , , , ,","place":["Detroit, Michigan, United States, 48201"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Devin L.","family":"Wakefield","sequence":"additional","affiliation":[{"name":"Cornell University , , , ,","place":["Ithaca, New York, United States, 14853"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Holowka","sequence":"additional","affiliation":[{"name":"Cornell University , , , ,","place":["Ithaca, New York, United States, 14853"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harold G.","family":"Craighead","sequence":"additional","affiliation":[{"name":"Cornell University , , , ,","place":["Ithaca, New York, United States, 14853"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara A.","family":"Baird","sequence":"additional","affiliation":[{"name":"Cornell University , , , ,","place":["Ithaca, New York, United States, 14853"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2014,7,8]]},"reference":[{"key":"2026090205053628000_cit1","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1038\/nrm2977","article-title":"Revitalizing Membrane Rafts: New Tools and Insights","volume":"11","author":"Simons","year":"2010","journal-title":"Nat. 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