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Struct."},{"key":"bi971167gb00001\/bi971167gb00001_1","volume-title":"bovine cerebrosides","author":"Abbreviations CB"},{"key":"bi971167gb00005\/bi971167gb00005_1","unstructured":"Gel phase is also resistant to detergent solubilization (Ribiero & Dennis, 1973) but is unlikely to occur in the cholesterol-rich plasma membrane. Interestingly, we found that SM alone was detergent-soluble at 23 \u00b0C despite being in the gel phase. This solubility is not a surprise, as insolubility of gel phases can require temperatures well belowTm(Ribiero & Dennis, 1973). We found that pure SM was insoluble at 4 \u00b0C (data not shown)."},{"key":"bi971167gb00006\/bi971167gb00006_1","unstructured":"The absolute decrease in light scattering when detergent was added was slightly variable, within the range 0\u221220% for DPPC\/cholesterol and SM\/cholesterol and 80\u2212100% for DOPC\/cholesterol and 12SLPC\/cholesterol. This variation appears to reflect an artifact in light scattering measurements. Centrifugation experiments previously demonstrated the total insolubility of 2:1 (mol\/mol) DPPC\/cholesterol and total solubility of 2:1 DOPC\/cholesterol (Schroeder et al., 1994). In addition, centrifugation and filtration experiments showed that 12SLPC\/cholesterol and DOPC\/cholesterol were equally detergent-soluble (unpublished observations)."}],"container-title":["Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/bi971167g","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,19]],"date-time":"2023-04-19T11:24:46Z","timestamp":1681903486000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/doi\/10.1021\/bi971167g"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,9,1]]},"references-count":63,"journal-issue":{"issue":"36","published-print":{"date-parts":[[1997,9,1]]}},"alternative-id":["10.1021\/bi971167g"],"URL":"https:\/\/doi.org\/10.1021\/bi971167g","relation":{},"ISSN":["0006-2960","1520-4995"],"issn-type":[{"value":"0006-2960","type":"print"},{"value":"1520-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,9,1]]}}}