{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T16:41:04Z","timestamp":1792687264303,"version":"4.5.0"},"reference-count":23,"publisher":"American Chemical Society (ACS)","license":[{"start":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:00:00Z","timestamp":1792627200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:00:00Z","timestamp":1792627200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:00:00Z","timestamp":1792627200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2024JJ6378"],"award-info":[{"award-number":["2024JJ6378"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32171270"],"award-info":[{"award-number":["32171270"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>It is of interest to develop new approaches to probing protein conformational changes and protein\u2013protein interactions. In this study, we covalently labeled human neuroglobin (Ngb) with a 19F-probe containing a Ph-OCF3 group at Cys120 and by introducing cysteine residues at specific positions in the C120S Ngb mutant. 19F NMR provides valuable insights into its conformational changes upon cleavage of the intramolecular disulfide bond of Cys46-Cys55, as well as its interactions with protein partners such as cytochrome c (Cyt c) through the formation of the Ngb-Cyt c complex. This study presents a convenient approach to investigating the dynamic properties of heme globins regulated by disulfide bonds and protein\u2013protein interactions.<\/jats:p>","DOI":"10.1021\/acs.biochem.6c00592","type":"journal-article","created":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T16:01:42Z","timestamp":1792684902000},"source":"Crossref","is-referenced-by-count":0,"title":["Covalent Labeling\nof Human Neuroglobin with a 19F-Probe to Study its\nConformational Changes Using 19F NMR"],"prefix":"10.1021","author":[{"given":"Si-Qi","family":"Xia","sequence":"first","affiliation":[{"name":"University of South China , , ,","place":["Hengyang, China, 421001"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi-Chun","family":"Liu","sequence":"additional","affiliation":[{"name":"University of South China , , ,","place":["Hengyang, China, 421001"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu-Qin","family":"Gao","sequence":"additional","affiliation":[{"name":"University of South China , , ,","place":["Hengyang, China, 421001"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Yuan","sequence":"additional","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, China, 200433"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangshi","family":"Tan","sequence":"additional","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, China, 200433"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-2127-0651","authenticated-orcid":true,"given":"Ying-Wu","family":"Lin","sequence":"additional","affiliation":[{"name":"University of South China , , ,","place":["Hengyang, China, 421001"]},{"name":"University of South China , , ,","place":["Hengyang, China, 421001"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,10,22]]},"reference":[{"issue":"6803","key":"2026102212013784900_cit1","doi-asserted-by":"publisher","first-page":"520","DOI":"10.1038\/35035093","article-title":"A vertebrate globin\nexpressed in the brain","volume":"407","author":"Burmester","year":"2000","journal-title":"Nature"},{"issue":"4","key":"2026102212013784900_cit2","doi-asserted-by":"publisher","first-page":"1005","DOI":"10.1107\/S1399004714000078","article-title":"The crystal\nstructure of wild-type human brain neuroglobin reveals flexibility\nof the disulfide bond that regulates oxygen affinity","volume":"70","author":"Guimaraes","year":"2014","journal-title":"Acta Crystallogr. 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